LOCUS D390pUbiZm-LmEGF 5641 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of GFP-Hind-Sal* into B633pUbiZm-LGFP* ACCESSION D390pUbiZm-LmEGF KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5641) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B633pUbiZm-LGFP* Fragment ends: SalI and HindIII Fragment size: 4662 Insert molecule: GFP-Hind-Sal* Fragment ends: HindIII and SalI Fragment size: 979 Sequencing primers: YFPDCR CCATCTTCCTTGAAATCGATAC LH1 CCCAAGATCTGGCCCTT FEATURES Location/Qualifiers misc_feature 106..1614 /gene="PUbi" /product="Ubi-int Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1093..1612 /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1664..1714 /gene="link" /SECDrawAs="Label" CDS 1715..2093 /gene="mEGFP" /product="mEGFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 2094..2282 /gene="ST-LS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 CDS 2283..2620 /gene="mEGFP" /product="mEGFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2519..2521 /product="A206K" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2630..2825 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3641..4501 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4544..5260 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctaggctgca gtgcagcgtg 121 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta 181 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat 241 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga 301 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag 361 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt 421 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg 481 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag 541 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa 601 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt 661 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgatcgac gagtctaacg 721 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat 781 ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc 841 tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc 901 tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct 961 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct 1021 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac 1081 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc 1141 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat 1201 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag 1261 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag 1321 ccgttccgca gacgggatcg atctaggata ggtatacatg ttgatgtggg ttttactgat 1381 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct 1441 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca 1501 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg 1561 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtactagt 1621 tggatccccc gggctgcagg aattcaagct tacgcgttgt acaggagtac cgccccgtcc 1681 ggtcctgccc gtcaccgaga tcagcggaga gttcatgggc aagggcgagg aactgttcac 1741 tggcgtggtc ccaatcctgg tggaactgga tggtgatgtg aacgggcaca agttctccgt 1801 cagcggagag ggtgaaggtg atgccaccta cggaaagctc accctgaagt tcatctgcac 1861 taccggaaag ctccctgttc cgtggccaac cctcgtcacc actttcacct acggtgttca 1921 gtgcttctcc cgttacccag atcacatgaa gcagcatgac ttcttcaaga gcgccatgcc 1981 cgaaggctac gtgcaagaaa ggactatctt cttcaaggat gacgggaact acaagacacg 2041 tgccgaagtc aagttcgaag gtgataccct ggtgaaccgc atcgagctga aaggtaagtt 2101 tctgcttcta cctttgatat atatataata attatcatta attagtagta atataatatt 2161 tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta 2221 taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc 2281 aggtatcgat ttcaaggaag atggaaacat cctcggacac aagctggagt acaactacaa 2341 ctcccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2401 gatcaggcac aacatcgaag atggaagcgt gcaactggcg gaccactacc agcagaacac 2461 gcccatcggc gatggccctg tcctgctgcc ggacaaccat tacctgtcca cgcaatctaa 2521 gctctccaag gaccccaacg agaagaggga ccacatggtc ctgctggagt tcgtgacggc 2581 tgctgggatc acgcatggca tggatgaact ctacaagtga cccggtcgac cctgctttaa 2641 tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt 2701 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg 2761 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat 2821 tgtcctcgac aggccttaag ggccagatct tgggcccggt acccaattcg ccctatagtg 2881 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 2941 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 3001 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt 3061 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 3121 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 3181 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 3241 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 3301 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 3361 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 3421 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 3481 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 3541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 3601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 3661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 3721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 3781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 3841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 3901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 3961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 4021 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 4081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 4141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 4201 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 4261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 4321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 4381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 4801 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 5281 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 5341 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 5401 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 5461 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 5521 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 5581 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 5641 t //