LOCUS D467pD35s-LmEGFP 4924 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of P2x35S* into D360p35s-LmEGFP* ACCESSION D467pD35s-LmEGFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4924) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: D360p35s-LmEGFP* Fragment ends: BamHI and NcoI Fragment size: 4106 Insert molecule: P2x35S* Fragment ends: NcoI and BamHI Fragment size: 818 Primers for gene synthesis PucSyn CGCCAAGCTCGAAATTAACCCTCAC GFPS GGATTGGGACCACGCCAGTGA Sequencing primers GFP-seq CCAGTTCCACCAGGATTG FEATURES Location/Qualifiers misc_feature 110..894 /gene="P35S" /product="P2x35s" /SECDrawAs="Region" /SECStyleId=1 misc_feature 111..436 /product="eh1" /SECDrawAs="Region" /SECStyleId=1 misc_feature 436..761 /product="enh2" /SECDrawAs="Region" /SECStyleId=1 misc_feature 724 /gene="mut " /SECDrawAs="Label" misc_feature 822..828 /gene="TATA" /SECDrawAs="Label" CDS 998..1376 /gene="mEGFP" /product="mEGFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1187..1192 /product="F64L/S65T - enhanced fluorescence" /SECDrawAs="Region" /SECStyleId=1 CDS 1377..1565 /gene="ST-LS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 CDS 1566..1903 /gene="mEGFP" /product="mEGFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1802..1804 /product="A206K - monomeric protein" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1913..2108 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2924..3784 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 3827..4543 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgct agatcgggcc aacatggtgg 121 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg 181 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag 241 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc 301 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg 361 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc 421 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa 481 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga 541 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag 601 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc 661 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 721 gaccttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 781 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 841 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgact 901 agtaggatcc cccgggctgc aggaattcaa gcttacgcgt tgtacaggag taccgccccg 961 tccggtcctg cccgtcaccg agatcagcgg agagttcatg ggcaagggcg aggaactgtt 1021 cactggcgtg gtcccaatcc tggtggaact ggatggtgat gtgaacgggc acaagttctc 1081 cgtcagcgga gagggtgaag gtgatgccac ctacggaaag ctcaccctga agttcatctg 1141 cactaccgga aagctccctg ttccgtggcc aaccctcgtc accactttca cctacggtgt 1201 tcagtgcttc tcccgttacc cagatcacat gaagcagcat gacttcttca agagcgccat 1261 gcccgaaggc tacgtgcaag aaaggactat cttcttcaag gatgacggga actacaagac 1321 acgtgccgaa gtcaagttcg aaggtgatac cctggtgaac cgcatcgagc tgaaaggtaa 1381 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat 1441 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt 1501 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg 1561 tgcaggtatc gatttcaagg aagatggaaa catcctcgga cacaagctgg agtacaacta 1621 caactcccac aacgtataca tcatggccga caagcagaag aacggcatca aggtgaactt 1681 caagatcagg cacaacatcg aagatggaag cgtgcaactg gcggaccact accagcagaa 1741 cacgcccatc ggcgatggcc ctgtcctgct gccggacaac cattacctgt ccacgcaatc 1801 taagctctcc aaggacccca acgagaagag ggaccacatg gtcctgctgg agttcgtgac 1861 ggctgctggg atcacgcatg gcatggatga actctacaag tgacccggtc gaccctgctt 1921 taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac 1981 gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta 2041 atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact 2101 gattgtcctc gacaggcctt aagggccaga tcttgggccc ggtacccaat tcgccctata 2161 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 2221 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 2281 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat 2341 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 2401 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 2461 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 2521 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 2581 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 2641 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 2701 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 2761 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg 2821 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 2881 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 2941 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 3001 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 3061 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 3121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 3181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 3241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 3301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 3361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 3421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 3481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 3541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 3601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 3661 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 3721 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 3781 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 3841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 4021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 4081 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 4141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 4201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 4441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 4501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 4561 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 4621 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 4681 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 4741 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 4801 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 4861 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 4921 attt //