LOCUS pLNU-GFP 5758 bp DNA circular SYN 13-JUL-2010 DEFINITION Ligation of L29GFPeag* into pLNU-BA* ACCESSION pLNU-GFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5758) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software FEATURES Location/Qualifiers misc_feature 91..124 /gene="loxP" /product="LoxP511" misc_feature 140..1648 /gene="Ubi-int" /product="Promotor" misc_feature 1127..1646 /gene="Intron" /product="Intron" misc_feature 1698..1700 /gene="(null)" /product="Start in MCS" CDS 1721..2099 /gene="GFP" /product="GFP" CDS 2100..2288 /gene="ST-LS1" /product="Intron" CDS 2289..2626 /gene="GFP" /product="GFP" misc_feature 2633..2886 /gene="Tnos" /product="NOS-Terminator" misc_feature 2893..2926 /gene="LoxP" /product="LoxP511" misc_feature complement (3412..4128) /gene="ColE1" /product="ColE1" CDS complement (4171..5031) /gene="Amp" /product="ORF-1" ORIGIN 1 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg 61 cccaagatct ggcccttagg gccttaatta ataacttcgt ataatgtata ctatacgaag 121 ttattgcggc cgcactaggc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat 181 aatgagcatt gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt 241 ttgaagtgca gtttatctat ctttatacat atatttaaac tttactctac gaataatata 301 atctatagta ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca 361 tggtctaaag gacaattgag tattttgaca acaggactct acagttttat ctttttagtg 421 tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt 481 attagtacat ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac 541 atctatttta ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt 601 ttatttaata atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata 661 ccctttaaga aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca 721 gcctgttaaa cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt 781 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct 841 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg 901 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag 961 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa 1021 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca 1081 caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc 1141 ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc 1201 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc 1261 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag 1321 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag 1381 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca 1441 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata 1501 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt 1561 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac 1621 cctgttgttt ggtgttactt ctgcaggtac tagtgggtac cagagctccc taggttctag 1681 aaccggtgac gtctcccatg gtgaagcttg gatccggccg atgggcaagg gcgaggaact 1741 gttcactggc gtggtcccaa tcctggtgga actggatggt gatgtgaacg ggcacaagtt 1801 ctccgtcagc ggagagggtg aaggtgatgc cacctacgga aagctcaccc tgaagttcat 1861 ctgcactacc ggaaagctcc ctgttccgtg gccaaccctc gtcaccactt tcacctacgg 1921 tgttcagtgc ttctcccggt acccagatca catgaagcag catgacttct tcaagagcgc 1981 catgcccgaa ggctacgtgc aagaaaggac tatcttcttc aaggatgacg ggaactacaa 2041 gacacgtgcc gaagtcaagt tcgaaggtga taccctggtg aaccgcatcg agctgaaagg 2101 taagtttctg cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat 2161 aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt 2221 agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg 2281 atgtgcaggt atcgatttca aggaagatgg aaacatcctc ggacacaagc tggagtacaa 2341 ctacaactcc cacaacgtat acatcatggc cgacaagcag aagaacggca tcaaggtgaa 2401 cttcaagatc aggcacaaca tcgaagatgg aagcgtgcaa ctggcggacc actaccagca 2461 gaacacgccc atcggcgatg gccctgtcct gctgccggac aaccattacc tgtccacgca 2521 atctgccctc tccaaggacc ccaacgagaa gagggaccac atggtcctgc tggagttcgt 2581 gacggctgct gggatcacgc atggcatgga tgaactctac aagtgactcg acgatcgttc 2641 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat 2701 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt 2761 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga 2821 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact 2881 agatcggtac ctataacttc gtataatgta tactatacga agttatttaa attggccccc 2941 atggcctcta gagagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc 3001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 3061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 3121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 3181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 3241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 3361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 3601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3841 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 3961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 4081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 4201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 4321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 4381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 4441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 4501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 4561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 4621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 4681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 4741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 4801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 4861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 4921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 4981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 5041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 5101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 5161 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 5581 taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg 5641 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 5701 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgt //