LOCUS       pNap-Nos-BM             3599 bp    DNA     circular SYN 14-JUL-2010
DEFINITION  Nap-Nos-BM rotated to position 3572
ACCESSION   pNap-Nos-BM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    33..396
                     /gene="Nap"
                     /product="Promotor"
     misc_feature    457..710
                     /gene="Tnos"
                     /product="NOS-T"
     misc_feature    1527..2387
                     /gene="Amp"
                     /product="Amp"
     misc_feature    2430..3146
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 gagctctcta gaggccatgg cggccgcact agaatttata gactctcatc cccttttaaa
       61 ccaacttagt aaacgttttt ttttttaatt ttatgaagtt aagtttttac cttgttttta
      121 aaaagaatcg ttcataagat gccatgccag aacattagct acacgttaca catagcatgc
      181 agccgcggag aattgttttt cttcgccact tgtcactccc ttcaaacacc taagagcttc
      241 tctctcacag cacacacata caatcacatg cgtgcatgca ttattacacg tgatcgccat
      301 gcaaatctcc tttatagcct ataaattaac tcatccgctt cactctttac tcaaaccaaa
      361 actcatcaat acaaacaaga ttaaaaacat acacgaacta gtggatcccc cgggctgcag
      421 gaattcaagc ttacgcgtgt cgactcgaat ttccccgatc gttcaaacat ttggcaataa
      481 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
      541 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
      601 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
      661 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ctcgacaggc
      721 cttaagggcc agatcttggg cccggtaccc aattcgccct atagtgagtc gtattacaat
      781 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      841 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      901 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt
      961 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     1021 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     1081 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     1141 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     1201 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     1261 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     1321 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     1381 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1441 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1501 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1561 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1621 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1681 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1741 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1801 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1861 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1921 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1981 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2041 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2101 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2161 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2221 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2281 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2341 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2401 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2461 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2701 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3181 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3241 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3301 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     3541 gctatgacca tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctg
//