LOCUS       pUbiH-Nos               3569 bp    DNA     circular SYN 14-JUL-2010
DEFINITION  Ligation of Ubi-Hel-Not-Spe* into p35-Nos-BM*
ACCESSION   pUbiH-Nos
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    129..464
                     /gene="Ubi"
                     /product="Promotor"
     misc_feature    525..778
                     /gene="Tnos"
                     /product="terminator"
     CDS             1595..2455
                     /gene="Amp"
                     /product="ORF-1"
     misc_feature    2498..3214
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcgacaa aacttaagaa agtcaccaaa aagaaagtca aagacggcaa cggcaaccca
      181 gaagctacgt tctctttcta gattctatgc cctacctacc gcgtggtgca caacaacttg
      241 acccccatca ttgactctcc accgtacacg tgtcacgata ccatttgttg gtctcctacc
      301 acttacgtaa cattaatatt ccatccgtga ccgcctcttc ttcatcatcc tataaattca
      361 ccttcttatc tcttccgctt tcattcgttc atcatcatca caattaacaa caatccaaag
      421 caatcttctc tcatattctt atctcttcct gtttcgcttc gtctactagt ggatcccccg
      481 ggctgcagga attcaagctt acgcgtgtcg actcgaattt ccccgatcgt tcaaacattt
      541 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
      601 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
      661 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
      721 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgct
      781 cgacaggcct taagggccag atcttgggcc cggtacccaa ttcgccctat agtgagtcgt
      841 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      901 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      961 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     1021 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     1081 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     1141 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     1201 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     1261 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     1321 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     1381 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     1441 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1501 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1561 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1621 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1681 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1741 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1801 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1861 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1921 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1981 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2041 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2101 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2161 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2221 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2281 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2341 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2401 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2461 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2521 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2581 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2641 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2701 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2761 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2821 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2881 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2941 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3001 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3061 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3121 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3181 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3241 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3301 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3361 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3421 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     3481 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     3541 acactttatg cttccggctc gtatgttgt
//