LOCUS B728pUbiZm-YFPiM 5583 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of PCR1* into B595pUbiZm-mGFPM-ocs* ACCESSION B728pUbiZm-YFPiM KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5583) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B595pUbiZm-mGFPM-ocs* Fragment ends: SalI and MluI Fragment size: 4654 Insert molecule: PCR1* Fragment ends: MluI and SalI Fragment size: 929 Sequencing primers: Ubim M13FL pUCF GCAAGGCGATTAAGTTGGGTA optional statt M13FL FEATURES Location/Qualifiers misc_feature 122..1629 /gene="pUbi" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1108..1627 /gene="Int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 CDS 1655..2036 /gene="YFP'" /product="mCtrine (codon optimized for plants)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mCitrine " /SECDescr="mCitrine (codon optimized for plants)" CDS 2037..2225 /gene="Int" /product="ST-LS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2226..2563 /gene="'YFP" /product="mCtrine (codon optimized for plants)" /codon_start=2 /translation="YRFQGRWKYSRTQVGVQLQQP" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mCitrine " /SECDescr="mCitrine (codon optimized for plants)" misc_feature 2572..2767 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 3583..4443 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4486..5202 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtac 121 gtgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 181 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 241 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 301 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 361 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 421 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 481 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 541 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 601 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 661 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 721 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag 781 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg 841 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg 901 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc 961 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc 1021 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa 1081 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc 1141 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc 1201 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg 1261 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc 1321 ctgggatggc tctagccgtt ccgcagacgg gatcgatcta ggataggtat acatgttgat 1381 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac 1441 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat 1501 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg 1561 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact 1621 tctgcaggta caagcttacg cgtcctccac cacaatggtg tccaagggcg aggagctgtt 1681 caccggagtc gttcccatcc tcgtcgaatt ggacggcgac gtgaacggtc acaagttcag 1741 cgtgtctggg gagggcgagg gagatgccac ctacggcaag ctcactctca agttcatttg 1801 caccaccgga aagctgccag tcccttggcc gaccctcgtt actaccttcg gctacggttt 1861 gatgtgcttc gctcgctacc ccgaccacat gaagcagcac gacttcttta agtccgccat 1921 gccagagggc tacgtccaag agaggaccat cttcttcaag gacgatggga actacaagac 1981 ccgcgccgaa gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaaaggtaa 2041 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat 2101 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt 2161 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg 2221 tgcaggtatc gatttcaagg aagatggaaa tattctcgga cacaagttgg agtacaacta 2281 caacagccat aacgtgtata tcatggctga caagcagaag aacggcatca aagtcaactt 2341 caagatcagg cacaatattg aggatggttc ggtgcagctc gccgaccact accagcaaaa 2401 cactcctatc ggggacggcc cggttcttct ccccgacaac cactacctgt cctaccagag 2461 cgccctctcc aaggacccaa acgaaaagcg cgatcacatg gtcttgcttg agttcgtgac 2521 cgctgccgga atcaccctgg gcatggacga gctttacaag tgactcgtcg accctgcttt 2581 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg 2641 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa 2701 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg 2761 attgtcctcg acaggcctta agggccagat cttgggcccg gtacccaatt cgccctatag 2821 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 2881 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 2941 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt 3001 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 3061 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 3121 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 3181 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 3241 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 3301 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 3361 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 3421 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 3481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 3541 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 3601 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 3661 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 3721 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 3781 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 3841 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 3901 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 3961 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4021 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4081 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 4141 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 4201 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 4261 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 4321 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 4381 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 4441 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 4501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4741 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 4801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 4861 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 4921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 4981 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5221 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 5281 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 5341 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 5401 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 5461 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 5521 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 5581 ttt //