LOCUS B893p35S-CheriD- 4483 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of cheri-Mlu-Sal* into B892p35S-CherD-ocs* ACCESSION B893p35S-CheriD- KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4483) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B892p35S-CherD-ocs* Fragment ends: SalI and MluI Fragment size: 3563 Insert molecule: cheri-Mlu-Sal* Fragment ends: MluI and SalI Fragment size: 920 Sequencing primers: 35S GCACAATCCCACTATCCTT FEATURES Location/Qualifiers misc_feature 144..562 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="35S-Pro" /SECDescr="Promotor-Region" misc_feature 473..562 /gene="Minimal promotor" /SECDrawAs="Label" CDS 590..969 /gene="mCherry'" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mCherry" CDS 987..1156 /gene="Int" /product="ST-LS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1168..1489 /gene="'mCherry" /codon_start=2 /translation="GTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKD GGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMD ELYK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mCherry" misc_feature 1498..1693 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 2509..3369 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3412..4128 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa 181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt 241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac 301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt 361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt 421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac 481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg 541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tggtgtccaa 601 gggcgaggag gacaacatgg ccatcattaa ggagttcatg cgcttcaaag tgcacatgga 661 aggaagcgtt aacggccacg agttcgagat cgagggtgag ggagagggtc gtccctacga 721 aggaacccag actgctaagc tcaaagtcac caagggcgga ccattgcctt tcgcctggga 781 catcttgtcc ccgcaattta tgtacggctc taaggcatac gtgaagcacc ccgccgatat 841 tccagactac cttaagctct cattccccga gggtttcaag tgggagaggg tcatgaactt 901 cgaggacgga ggcgtggtta ccgtcacaca ggactccagc ttgcaggatg gcgagttcat 961 ctacaaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt 1021 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc 1081 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa 1141 atttgttgat gtgcaggtga agctccgcgg gaccaatttc ccatcggacg gccctgtcat 1201 gcagaagaag actatgggat gggaggcttc cagtgaaaga atgtatcccg aggacggcgc 1261 cctgaaggga gagatcaagc aacgcctcaa gttgaaggac ggcggtcatt acgacgcgga 1321 agtgaagacg acctacaagg ccaagaagcc ggttcagctt ccaggcgcct acaacgtcaa 1381 catcaagctg gacatcacca gccacaacga ggactacact atcgtggagc agtacgagcg 1441 tgctgaggga aggcactcca ccggaggcat ggacgagctt tacaagtgac tcgtcgaccc 1501 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg 1561 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca 1621 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat 1681 ttactgattg tcctcgacag gccttaaggg ccagatcttg ggcccggtac ccaattcgcc 1741 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa 1801 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 1861 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 1921 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 1981 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 2041 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 2101 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 2161 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 2221 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 2281 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 2341 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 2401 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2461 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 2521 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 2581 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 2641 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 2701 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 2761 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 2821 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 2881 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 2941 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 3001 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 3061 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 3121 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 3181 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 3241 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 3301 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 3361 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 3421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 3481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 3541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 3601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 3661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 3721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 3781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 3841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 3901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 3961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 4021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 4081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 4141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 4201 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 4261 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 4321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 4381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 4441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgt //