LOCUS B982p935sGFP 10390 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of NptGFP-Xho-Mlu* into B414p7o-LH* ACCESSION B982p935sGFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10390) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B414p7o-LH* Fragment ends: MluI and XhoI Fragment size: 8426 Insert molecule: NptGFP-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 1964 Primer for gene synthesis NptXho cccggtCTCGAGtcctccataacaATGGGAGCATGGATTGA GFPMlu CCGCacgcGTCACTTGTAGAGTTCATC Sequencing primers: 35S GCACAATCCCACTATCCTT RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1043 /gene="NptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1044..1230 /gene="Int" /SECDrawAs="Gene" /SECStyleId=1 CDS 1231..1734 /gene="NptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1786..2164 /gene="GFPm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 CDS 2165..2353 /gene="Int" /product="ST-LS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2354..2691 /gene="GFPm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2701..2991 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3167..3190 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3725..4422 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4459..4718 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4971..8676 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8860..9870 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aacaatggga gcatggattg agcaggatgg cctccatgct 781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttcccggta 1741 ccgccccgtc cggtcctgcc cgtcaccgag atctccggag aattcatggg caagggcgag 1801 gaactgttca ctggcgtggt cccaatcctg gtggaactgg atggtgatgt gaacgggcac 1861 aagttctccg tcagcggaga gggtgaaggt gatgccacct acggaaagct caccctgaag 1921 ttcatctgca ctaccggaaa gctccctgtt ccgtggccaa ccctcgtcac cactttcacc 1981 tacggtgttc agtgcttctc ccggtaccca gatcacatga agcagcatga cttcttcaag 2041 agcgccatgc ccgaaggcta cgtgcaagaa aggactatct tcttcaagga tgacgggaac 2101 tacaagacac gtgccgaagt caagttcgaa ggtgataccc tggtgaaccg catcgagctg 2161 aaaggtaagt ttctgcttct acctttgata tatatataat aattatcatt aattagtagt 2221 aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt 2281 tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt 2341 tgttgatgtg caggtatcga tttcaaggaa gatggaaaca tcctcggaca caagctggag 2401 tacaactaca actcccacaa cgtatacatc atggccgaca agcagaagaa cggcatcaag 2461 gtgaacttca agatcaggca caacatcgaa gatggaagcg tgcaactggc ggaccactac 2521 cagcagaaca cgcccatcgg cgatggccct gtcctgctgc cggacaacca ttacctgtcc 2581 acgcaatctg ccctctccaa ggaccccaac gagaagaggg accacatggt cctgctggag 2641 ttcgtgacgg ctgctgggat cacgcatggc atggatgaac tctacaagtg acgcgttcga 2701 gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg 2761 tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa 2821 tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt 2881 gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg 2941 tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac tgaagcctgc 3001 aggcatgcaa gctgatccac tagaggccat ggcggccgca ctagtggatc ccccgggatg 3061 catgaattca agcttatcga tgtcgacagg ccttaagggc cagatcttgg gcccggtacc 3121 cgatcagatt gtcgtttccc gccttcggtt taaactatca gtgtttgaca ggatatattg 3181 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg 3241 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga 3301 gtgcttggca ttccgtgcga taatgacttc tgttcaacca cccaaacgtc ggaaagcctg 3361 acgacggagc agcattccaa aaagatccct tggctcgtct gggtcggcta gaaggtcgag 3421 tgggctgctg tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc 3481 ctcgatcgca aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac 3541 gtcctggcca gtcatcacgc gccaaagttc cgtcacagga tgatctggcg cgagttgctg 3601 gatctcgcct tcaatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa 3661 gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac 3721 gccgaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3781 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 3901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 3961 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 4021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 4081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 4321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 4381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 4441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 4501 ccctgattct gtggataacc gattaccgcc tttgagtgag ctgataccgc tcgccgcagc 4561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 4621 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 4681 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 4741 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 4801 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 4861 caccgtcatc accgaaacgc gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc 4921 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg 4981 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc 5041 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg 5101 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac 5161 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag 5221 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct 5281 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg 5341 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg 5401 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag 5461 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga 5521 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag 5581 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc 5641 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg 5701 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat 5761 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca 5821 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc 5881 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctcgcgg 5941 cctggccggc gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 6001 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 6061 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 6121 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 6181 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 6241 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 6301 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 6361 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 6421 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 6481 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 6541 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 6601 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 6661 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 6721 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 6781 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 6841 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 6901 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 6961 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 7021 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 7081 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 7141 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg 7201 gtggagaagt tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc 7261 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg 7321 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc 7381 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt 7441 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac 7501 gggcacgtag aggtttccgc agggccggcc ggcatggcga gtgtgtggga ttacgacctg 7561 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga 7621 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga 7681 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg 7741 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag 7801 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac 7861 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac 7921 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac 7981 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac 8041 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc 8101 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc 8161 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg 8221 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct 8281 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt 8341 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag 8401 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc 8461 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc 8521 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc 8581 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg 8641 tcgccactcg accgccggcg cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg 8701 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg 8761 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca 8821 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc 8881 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 8941 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 9001 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 9061 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 9121 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 9181 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 9241 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 9301 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 9361 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 9421 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 9481 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 9541 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 9601 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 9661 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 9721 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 9781 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 9841 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 9901 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 9961 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 10021 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat 10081 cgcaacatag cttgcttggt cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa 10141 atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg 10201 cttctctcgc aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt 10261 gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta 10321 tctgcctaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag 10381 cggcagcggc //