LOCUS B983p9UbiGfp 11449 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of NptGFP-Xho-Mlu* into B434p7oU10-LH* ACCESSION B983p9UbiGfp KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11449) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B434p7oU10-LH* Fragment ends: MluI and XhoI Fragment size: 9485 Insert molecule: NptGFP-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 1964 Primer for gene synthesis NptXho cccggtCTCGAGtcctccataacaATGGGAGCATGGATTGA GFPMlu CCGCacgcGTCACTTGTAGAGTTCATC Sequencing primers: Ubq10-F CTAGATCTGGTGTTAGTTTCTAGT RBCS-TR2 CACAGTAAATTACAAGCACAAC GFP-seq CCAGTTCCACCAGGATTG optional E9Mlu CCCAATGCCATAATACTCGAACGCG FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="Pro-Ubi" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="Int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2102 /gene="NptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 2103..2289 /gene="Int" /SECDrawAs="Gene" /SECStyleId=1 CDS 2290..2793 /gene="NptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 2845..3223 /gene="GFPm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 CDS 3224..3412 /gene="Int" /product="ST-LS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 3413..3750 /gene="GFPm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3760..4050 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4226..4249 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4784..5481 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5518..5777 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6030..9735 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9919..10929 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 acaatgggag catggattga gcaggatggc ctccatgctg gtagccccgc tgcttgggtg 1861 gagcgcctct ttggctacga ctgggcccaa cagaccattg ggtgcagtga tgccgctgtc 1921 ttccgtttga gcgcccaggg caggccagtt ctcttcgtga agactgacct ctccggtgcc 1981 ctcaacgagt tgcaggacga agctgcccgc ctctcatggc ttgccaccac cggagtccct 2041 tgcgctgccg tgttggacgt tgtcacagag gccggaagag actggctgct cttgggcgag 2101 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata 2161 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg 2221 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt 2281 gatgtgcagg tgccaggcca agacctcctg agctcccacc tggccccagc cgagaaggtc 2341 tccatcatgg cagacgccat gcgtcgcctc cacaccctgg acccagccac ctgccccttc 2401 gaccaccagg ccaagcacag gatcgaacgt gcacgcacta ggatggaggc cggccttgtg 2461 gaccaggatg accttgacga ggagcaccag ggccttgctc ccgccgagct gttcgcccgc 2521 ctcaaggcta gaatgcctga cggagaggac ttggtggtca cccacgggga tgcctgcctc 2581 cccaacatca tggttgagaa cggacgcttc tctggcttca tcgactgcgg tcgtctgggc 2641 gtggccgacc gctaccaaga catcgccctg gccaccaggg acatcgctga ggaacttgga 2701 ggggagtggg ccgatcgttt cttggtcctc tacggcattg ctgcccccga ctcccagagg 2761 atcgccttct accgcctgct tgacgagttc ttcccggtac cgccccgtcc ggtcctgccc 2821 gtcaccgaga tctccggaga attcatgggc aagggcgagg aactgttcac tggcgtggtc 2881 ccaatcctgg tggaactgga tggtgatgtg aacgggcaca agttctccgt cagcggagag 2941 ggtgaaggtg atgccaccta cggaaagctc accctgaagt tcatctgcac taccggaaag 3001 ctccctgttc cgtggccaac cctcgtcacc actttcacct acggtgttca gtgcttctcc 3061 cggtacccag atcacatgaa gcagcatgac ttcttcaaga gcgccatgcc cgaaggctac 3121 gtgcaagaaa ggactatctt cttcaaggat gacgggaact acaagacacg tgccgaagtc 3181 aagttcgaag gtgataccct ggtgaaccgc atcgagctga aaggtaagtt tctgcttcta 3241 cctttgatat atatataata attatcatta attagtagta atataatatt tcaaatattt 3301 ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta 3361 tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc aggtatcgat 3421 ttcaaggaag atggaaacat cctcggacac aagctggagt acaactacaa ctcccacaac 3481 gtatacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatcaggcac 3541 aacatcgaag atggaagcgt gcaactggcg gaccactacc agcagaacac gcccatcggc 3601 gatggccctg tcctgctgcc ggacaaccat tacctgtcca cgcaatctgc cctctccaag 3661 gaccccaacg agaagaggga ccacatggtc ctgctggagt tcgtgacggc tgctgggatc 3721 acgcatggca tggatgaact ctacaagtga cgcgttcgag tattatggca ttgggaaaac 3781 tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc 3841 tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt 3901 tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat 3961 tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat 4021 gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag ctgatccact 4081 agaggccatg gcggccgcac tagtggatcc cccgggatgc atgaattcaa gcttatcgat 4141 gtcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg 4201 ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa 4261 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc 4321 atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat 4381 aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa 4441 aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc 4501 ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct 4561 gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg 4621 ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt 4681 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt 4741 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa 4801 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 4861 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 4921 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 4981 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 5041 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 5101 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 5161 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5221 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5281 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5341 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5401 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5461 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5521 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 5641 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc 5701 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 5761 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc 5821 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 5881 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 5941 cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc 6001 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga 6061 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag 6121 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc 6181 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa 6241 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac 6301 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc 6361 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag 6421 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc 6481 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc 6541 gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc 6601 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc 6661 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt 6721 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac 6781 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca 6841 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg 6901 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc 6961 atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg 7021 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc 7081 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg 7141 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac 7201 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc 7261 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg 7321 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt 7381 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt 7441 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga 7501 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc 7561 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga 7621 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc 7681 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct 7741 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa 7801 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg 7861 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat 7921 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct 7981 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt 8041 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg 8101 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc 8161 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc 8221 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg 8281 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg 8341 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt 8401 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg 8461 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac 8521 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca 8581 gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat 8641 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc 8701 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa 8761 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg 8821 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc 8881 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat 8941 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat 9001 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca 9061 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga 9121 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag 9181 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac 9241 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt 9301 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg 9361 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt 9421 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc 9481 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct 9541 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta 9601 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta 9661 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc 9721 ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg 9781 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc 9841 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag 9901 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc 9961 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag 10021 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta 10081 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg 10141 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag 10201 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc 10261 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca 10321 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag 10381 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat 10441 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc 10501 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat 10561 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat 10621 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc 10681 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa 10741 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag 10801 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg 10861 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc 10921 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca 10981 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt 11041 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg 11101 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc 11161 gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg 11221 cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg 11281 acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg 11341 gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc 11401 tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggc //