LOCUS C075p5oUZmF 11796 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of UZmF-Bsa* into B621p5oAct-LH2* ACCESSION C075p5oUZmF KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11796) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B621p5oAct-LH2* Fragment ends: XhoI and XbaI Fragment size: 9786 Insert molecule: UZmF-Bsa* Fragment ends: BsaI Fragment size: 2010 Primer for gene synthesis BsaXba CCCggtctCTCTAGACACTAGGCT BsaXho CCggtctcCTCGAGTGTACCTG Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC UF1 CGAGTAGATAATGCCAGCC UF2 GGCCCGGTAGTTCTACTTCT Ubim CCTGCCTTCATACGCTATTT LB2 CTACATGGATCAGCAATGAGTAT gc low FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 316..2309 /gene="UbiZmF" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 2331..2543 /gene="CTP" /product="CTP epsp Oryza sativa" /SECDrawAs="Gene" /SECStyleId=1 CDS 2544..3065 /gene="EPSPS" /codon_start=2 /translation="CTVPPAVRRPPGSLPASRGPSESPETRAFPTAHSCSAAWPAERQ GSPAFWRVRMSSTPGRLCRLWEPASGRKATRGLSTAWETAVSSPQRHPWTSATPPPDA V" /SECDrawAs="Gene" /SECStyleId=1 /SECName="EPSPS" misc_feature 3066..3254 /gene="STLS1" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Int" /SECDescr="STLS1" CDS 3255..4097 /gene="EPSPS" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 4107..4397 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4573..4596 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5131..5828 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6377..10082 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 10266..11276 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ctaggctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg 361 agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga 421 agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct 481 atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt 541 ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca 601 tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc cattttatta 661 gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct 721 attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat 781 ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct 841 ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct 901 gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg 961 gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt 1021 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca 1081 gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcacggca gctacggggg 1141 attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc 1201 cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat 1261 ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc 1321 cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct 1381 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg 1441 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct 1501 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt 1561 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac 1621 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg 1681 ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta 1741 attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat 1801 ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag 1861 agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt 1921 tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg 1981 tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta 2041 ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc 2101 atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat 2161 aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt 2221 tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca 2281 ccctgttgtt tggtgttact tctgcaggta cactcgagtc ctccaccgaa atggcctcca 2341 acgctgccgc cgcagccgct gtcagcttgg accaagccgt ggcggcctct gctgccttct 2401 cctcacgcaa gcagctccgt ctgcccgccg ctgccagggg tggcatgaga gttcgcgtcc 2461 gcgcccgtgg aaggcgcgag gcagtggttg tcgccagcgc ttccagctcg tccgtggccg 2521 cgccagccgc taaggccgaa gagttgcacg gtgcctccag ccgtccggcg accgccagga 2581 agtcttccgg cctctcgggg accgtcagaa tccccggaga caagagcatt tcccaccgct 2641 cattcatgtt cggcggcctg gccagcggag agacaaggat caccggcctt ctggagggtg 2701 aggatgtcat caacaccggg aaggctatgc aggctatggg agcccgcatc cggaaggaag 2761 gcgacacgtg gattatcgac ggcgtgggaa acggcggtct cctcgcccca gaggcaccct 2821 tggacttcgg caacgccgcc accggatgcc gtctgactat ggggcttgtt ggcgtgtacg 2881 atttcgactc caccttcatc ggagacgctt ccctcaccaa gcgccctatg ggcagggtcc 2941 taaatccgct ccgtgagatg ggcgtgcaag tcaagagcga ggacggagac cgcctgccag 3001 ttaccttgcg aggccccaag actccaacac ccatcaccta cagggtgcct atggcctctg 3061 cgcaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag 3121 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt 3181 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat 3241 ttgttgatgt gcaggtcaag tccgccgtgc tccttgccgg gctgaacact ccgggtatta 3301 ccaccgtcat cgagcccatt atgaccagag atcataccga gaagatgctc cagggattcg 3361 gcgctaactt gacggttgaa accgacgccg acggcgtgcg caccatccgt ctggagggaa 3421 ggggcaaact caccggccaa gtcatcgacg tgccaggaga tcccagctcg actgcttttc 3481 cacttgtagc cgccctcttg gtccctggtt ccgacgtgac catcctgaac gttcttatga 3541 acccgacccg caccgggctc atcttgactc tccaggagat gggcgctgac atcgaagtca 3601 ttaaccccag gctggccgga ggcgaggacg tggcagattt gcgcgtccgt agttccaccc 3661 tgaagggtgt gacagttcca gaggaccggg ctcccagcat gatcgacgag taccctatcc 3721 ttgccgtcgc cgcggccttc gccgagggcg ctaccgtgat gaacggattg gaggagctgc 3781 gcgtcaagga gtcagacaga ctctccgccg tggccaatgg gctcaagctc aacggcgttg 3841 actgcgatga gggagaaacc agccttgtcg tgcgtggcag gccggacggc aagggactcg 3901 gtaacgcttc tggggccgca gtcgccacgc acctggacca ccgcatcgcg atgtccttcc 3961 tcgtgatggg ccttgtttcg gagaacccag tcaccgtgga cgatgccacc atgattgcta 4021 ctagcttccc cgagttcatg gacttgatgg ccggactggg agccaagatc gagctgtccg 4081 acaccaaggc tgcctaacgc gttcgagtat tatggcattg ggaaaactgt ttttcttgta 4141 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga 4201 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt 4261 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg 4321 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat 4381 atgaggagta aaacactgaa gcctgcaggc atgcaagctg atccactaga ggccatggcg 4441 gccgcactag tggatccccc gggatgcatg aattcaagct tatcgatgtc gacaggcctt 4501 aagggccaga tcttgggccc ggtacccgat cagattgtcg tttcccgcct tcggtttaaa 4561 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag 4621 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca 4681 tgccaaccac agggttcccc tcgggagtgc ttggcattcc gtgcgataat gacttctgtt 4741 caaccaccca aacgtcggaa agcctgacga cggagcagca ttccaaaaag atcccttggc 4801 tcgtctgggt cggctagaag gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg 4861 cggacgtagc gcagcgccga aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg 4921 atctgcttgt cgctctttcg gccgacgtcc tggccagtca tcacgcgcca aagttccgtc 4981 acaggatgat ctggcgcgag ttgctggatc tcgccttcaa tccgggtctg tggcgggaac 5041 tccacgaaaa tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag 5101 tcgccgccga cgccgttgat gtggacgccg aaaaggatct aggtgaagat cctttttgat 5161 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5221 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5281 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5341 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 5401 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5461 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5521 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5581 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5641 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5701 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5761 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5821 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5881 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgatt accgcctttg 5941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 6001 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 6061 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac 6121 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga 6181 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 6241 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta 6301 cgtcgaggtc gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc 6361 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc 6421 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg 6481 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg 6541 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg 6601 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg 6661 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc 6721 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg 6781 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt 6841 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga 6901 gcgggcgcga ggccgccaag gcgcgaggcg tgaagtttgg cccccgccct accctcaccc 6961 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg 7021 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg 7081 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg 7141 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca 7201 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc 7261 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc 7321 cggtttgtct gatgccaagc tcgcggcctg gccggcgagc ttggccgctg aagaaaccga 7381 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc 7441 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc 7501 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc 7561 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc 7621 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg 7681 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg 7741 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg 7801 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag 7861 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa 7921 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt 7981 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt 8041 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt 8101 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc 8161 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag 8221 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga 8281 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc 8341 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga 8401 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga 8461 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact 8521 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa 8581 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggcggcgcag gccgcccagc 8641 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa 8701 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca 8761 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata 8821 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg 8881 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca 8941 tggcgagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca 9001 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg 9061 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag 9121 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga 9181 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa 9241 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg 9301 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg 9361 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag 9421 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt 9481 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg 9541 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg 9601 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg 9661 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt 9721 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac 9781 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa 9841 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag 9901 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt 9961 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc 10021 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca 10081 ccggtgggta tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg 10141 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac 10201 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt 10261 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 10321 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg 10381 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 10441 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga 10501 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac 10561 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca 10621 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt 10681 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg 10741 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg 10801 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca 10861 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg 10921 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc 10981 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg 11041 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg 11101 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg 11161 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt 11221 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc 11281 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca 11341 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa 11401 gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt 11461 gttatcgcaa tagttggcga agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac 11521 gtcggctcga ttgtacctgc gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg 11581 cgtcggctga tctcacggat cgactgcttc tctcgcaacg ccatccgacg gatgatgttt 11641 aaaagtccca tgtggatcac tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca 11701 catggctcag ttctcaatgg aaattatctg cctaaccggc tcagttctgc gtagaaacca 11761 acatgcaagc tccaccgggt gcaaagcggc agcggc //