LOCUS C138p6oAct-LH-no 10930 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of pNos-BM* into B488p6oAct-LH* corrected sequence ACCESSION C138p6oAct-LH-no KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10930) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B488p6oAct-LH* Fragment ends: SfiI Fragment size: 10595 Insert molecule: pNos-BM* Fragment ends: SfiI Fragment size: 336 Sequencing primers: TrbcsF GGCCTCTAATGACCGAAGTTAAT optional SBx GGTCCTTTTGTTCATTCTCAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 313..1710 /gene="pAct" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1244..1247 /gene="splice" /SECDrawAs="Label" misc_feature 1706..1709 /gene="splice" /SECDrawAs="Label" CDS 1738..2307 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2308..2496 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Int" /SECDescr="Intron StLS1" CDS 2497..2952 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2970..3260 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3369..3621 /gene="NOS-T" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 /SECName="NOS-T" /SECDescr="NOS-T" misc_feature 3707..3730 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4265..4962 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5511..9216 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9400..10410 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg 1741 aagaaaccgg agctgaccgc cacttccgtc gaaaagttcc tcatcgagaa gtttgatagc 1801 gtgtctgacc ttatgcagtt gtcggagggc gaagagtccc gcgcgttcag ctttgatgtt 1861 ggcgggcgcg ggtacgtcct ccgcgtgaac tcttgcgctg acggcttcta taaagatcgg 1921 tacgtttaca ggcatttcgc ctcggcggcc ctgcctattc ccgaagtcct cgacatcggc 1981 gagttttccg agagccttac gtattgtatt tcccgccggg ctcaaggggt gaccctccag 2041 gatctgccgg aaactgagtt gcctgcggtt ctccagcccg tcgccgaggc tatggacgcc 2101 atcgccgcgg cggatctttc tcaaacgtcg ggcttcgggc cgttcggccc tcaggggatt 2161 ggccagtaca ccacttggag ggactttatc tgcgctatcg ccgatccgca cgtgtatcat 2221 tggcaaaccg ttatggacga tacggtctcc gcgagcgtgg cccaggctct ggacgaactc 2281 atgttgtggg ccgaggattg ccccgaggta agtttctgct tctacctttg atatatatat 2341 aataattatc attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga 2401 atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac 2461 ttttctaata tatgaccaaa atttgttgat gtgcaggtcc gccacctggt tcatgcggac 2521 ttcggctcta ataacgtgct caccgataat gggcggatta ctgccgtcat cgactggtcc 2581 gaagctatgt ttggcgattc gcagtacgaa gtggcgaaca ttttcttctg gaggccttgg 2641 cttgcctgca tggaacagca aacgcgctac tttgagcgga ggcacccgga gctggcgggg 2701 tctccccgcc tcagggccta tatgttgcgg atcggcctcg accagcttta ccagtccctg 2761 gttgatggga atttcgacga tgctgcctgg gcgcaaggcc gctgtgacgc tattgtcagg 2821 agcggcgcgg ggaccgtggg ccgcactcag atcgcccggc ggtcggctgc cgtttggacg 2881 gatgggtgcg tcgaagtgct cgcggacagc ggcaacaggc ggccttccac ccgcccgcgg 2941 gccaaggagt gatccggaat cgcgttcgag tattatggca ttgggaaaac tgtttttctt 3001 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg 3061 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa 3121 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat 3181 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt 3241 aatatgagga gtaaaacact gaagcctgca ggcatgcaag ctgatccact agaggccatg 3301 gcggccgcac tagtggatcc cccgggctgc aggaattcaa gcttacgcgt gtcgactcga 3361 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 3421 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca 3481 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 3541 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 3601 tgtcatctat gttactaatc gctcgacagg ccttaagggc cagatcttgg gcccggtacc 3661 cgatcagatt gtcgtttccc gccttcggtt taaactatca gtgtttgaca ggatatattg 3721 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg 3781 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga 3841 gtgcttggca ttccgtgcga taatgacttc tgttcaacca cccaaacgtc ggaaagcctg 3901 acgacggagc agcattccaa aaagatccct tggctcgtct gggtcggcta gaaggtcgag 3961 tgggctgctg tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc 4021 ctcgatcgca aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac 4081 gtcctggcca gtcatcacgc gccaaagttc cgtcacagga tgatctggcg cgagttgctg 4141 gatctcgcct tcaatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa 4201 gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac 4261 gccgaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 4321 taagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 4381 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 4441 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 4501 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 4561 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 4621 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4681 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4741 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4801 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 4861 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 4921 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 4981 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 5041 ccctgattct gtggataacc gattaccgcc tttgagtgag ctgataccgc tcgccgcagc 5101 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 5161 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 5221 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 5281 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 5341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 5401 caccgtcatc accgaaacgc gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc 5461 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg 5521 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc 5581 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg 5641 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac 5701 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag 5761 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct 5821 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg 5881 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg 5941 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag 6001 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga 6061 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag 6121 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc 6181 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg 6241 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat 6301 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca 6361 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc 6421 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctcgcgg 6481 cctggccggc gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 6541 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 6601 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 6661 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 6721 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 6781 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 6841 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 6901 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 6961 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 7021 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 7081 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 7141 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 7201 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 7261 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 7321 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 7381 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 7441 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 7501 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 7561 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 7621 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 7681 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg 7741 gtggagaagt tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc 7801 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg 7861 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc 7921 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt 7981 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac 8041 gggcacgtag aggtttccgc agggccggcc ggcatggcga gtgtgtggga ttacgacctg 8101 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga 8161 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga 8221 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg 8281 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag 8341 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac 8401 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac 8461 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac 8521 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac 8581 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc 8641 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc 8701 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg 8761 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct 8821 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt 8881 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag 8941 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc 9001 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc 9061 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc 9121 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg 9181 tcgccactcg accgccggcg cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg 9241 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg 9301 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca 9361 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc 9421 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 9481 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 9541 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 9601 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 9661 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 9721 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 9781 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 9841 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 9901 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 9961 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 10021 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 10081 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 10141 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 10201 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 10261 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 10321 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 10381 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 10441 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 10501 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 10561 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat 10621 cgcaacatag cttgcttggt cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa 10681 atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg 10741 cttctctcgc aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt 10801 gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta 10861 tctgcctaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag 10921 cggcagcggc //