LOCUS C260pUbiZm-YFP 5394 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of YFPoi* into B595pUbiZm-mGFPM-ocs* ACCESSION C260pUbiZm-YFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5394) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B595pUbiZm-mGFPM-ocs* Fragment ends: SalI and MluI Fragment size: 4654 Insert molecule: YFPoi* Fragment ends: MluI and SalI Fragment size: 740 sequencing primer: Ubim CCTGCCTTCATACGCTATTT gc rich FEATURES Location/Qualifiers misc_feature 122..1629 /gene="pUbi" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1108..1627 /gene="Int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 CDS 1655..2374 /gene="YFP" /product="mCtrine (codon optimized for plants)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mCitrine " /SECDescr="mCtirine (codon optimized for plants)" misc_feature 2383..2578 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 3394..4254 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4297..5013 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtac 121 gtgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 181 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 241 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 301 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 361 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 421 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 481 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 541 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 601 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 661 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 721 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag 781 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg 841 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg 901 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc 961 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc 1021 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa 1081 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc 1141 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc 1201 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg 1261 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc 1321 ctgggatggc tctagccgtt ccgcagacgg gatcgatcta ggataggtat acatgttgat 1381 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac 1441 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat 1501 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg 1561 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact 1621 tctgcaggta caagcttacg cgtcctccac cgaaatggtg tccaagggcg aggagctgtt 1681 caccggagtc gttcccatcc tcgtcgaatt ggacggcgac gtgaacggtc acaagttcag 1741 cgtgtctggg gagggcgagg gagatgccac ctacggcaag ctcactctca agttcatttg 1801 caccaccgga aagctgccag tcccttggcc gaccctcgtt actaccttcg gctacggttt 1861 gatgtgcttc gctcgctacc ccgaccacat gaagcagcac gacttcttta agtccgccat 1921 gccagagggc tacgtccaag agaggaccat cttcttcaag gacgatggga actacaagac 1981 ccgcgccgaa gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaaaggtat 2041 cgatttcaag gaagatggaa atattctcgg acacaagttg gagtacaact acaacagcca 2101 taacgtgtat atcatggctg acaagcagaa gaacggcatc aaagtcaact tcaagatcag 2161 gcacaatatt gaggatggtt cggtgcagct cgccgaccac taccagcaaa acactcctat 2221 cggggacggc ccggttcttc tccccgacaa ccactacctg tcctaccaga gcgccctctc 2281 caaggaccca aacgaaaagc gcgatcacat ggtcttgctt gagttcgtga ccgctgccgg 2341 aatcaccctg ggcatggacg agctttacaa gtgactcgtc gaccctgctt taatgagata 2401 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa 2461 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat 2521 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc 2581 gacaggcctt aagggccaga tcttgggccc ggtacccaat tcgccctata gtgagtcgta 2641 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 2701 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 2761 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt 2821 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 2881 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 2941 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 3001 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 3061 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 3121 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 3181 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 3241 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 3301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 3361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 3421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 3481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 3541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 3601 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 3661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 3721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 3781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 3841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 3901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 3961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 4261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 4321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 4381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4561 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 4741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 4801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 4861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 4981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 5221 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 5281 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 5341 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca attt //