LOCUS C261p35s-LmRuby3 4361 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of mRubyNew* into B629p35s-LGFP* ACCESSION C261p35s-LmRuby3 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4361) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B629p35s-LGFP* Fragment ends: SalI and BsrGI Fragment size: 3586 Insert molecule: mRubyNew* Fragment ends: BsrGI and SalI Fragment size: 775 GELR Sequencing primers: 35RT CTTCGCAAGACCCTTCCTCT FEATURES Location/Qualifiers misc_feature 106..528 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="35S-Pro" /SECDescr="Promotor-Region" CDS 627..1337 /gene="ORF-1" /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQT MRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYED GGVVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRG YTDIALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQR EVAVAKYSNLGGGMDELYK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mRuby" misc_feature 1350..1545 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 2361..3221 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 3264..3980 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc 121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccc 541 ccgggctgca ggaattcaag cttacgcgtt gtacaggagt accgccccgt ccggtcctgc 601 ccgtcaccga gatcagcgga gagttcatgg tgtccaaggg cgaggagctg atcaaggaga 661 acatgaggat gaaagtcgtt atggagggaa gcgtgaacgg tcaccagttc aagtgcaccg 721 gcgagggaga ggggcgtcca tacgagggag tccaaactat gaggatcaaa gtgattgaag 781 gaggcccact ccctttcgcc ttcgacatct tggctacctc tttcatgtac ggctcccgca 841 ccttcatcaa gtaccccgcc gatatcccgg acttctttaa gcagtcattc ccagagggtt 901 tcacctggga gagagtcaca cgttacgagg acggcggagt tgtgaccgtc actcaggaca 961 ccagcctgga ggatggcgag ctggtttaca atgtgaaagt ccgcggggtg aacttcccct 1021 ccaacggacc tgtcatgcaa aagaagacca agggctggga accgaacacg gagatgatgt 1081 atccagccga cggtggcctc aggggataca ccgacattgc actcaaagtg gacggcggcg 1141 ggcacttgca ttgcaacttc gttaccactt accgctcgaa gaagaccgtc ggaaatatca 1201 agatgcccgg agtgcacgcc gtcgatcacc gtcttgagag gatcgaggag agcgacaacg 1261 aaacctacgt ggttcagcgc gaagtcgctg tggccaagta ctccaacctg ggtggaggca 1321 tggacgagct ttacaagtga cccggtcgac cctgctttaa tgagatatgc gagacgccta 1381 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg 1441 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat 1501 cgtattttta tgaataatat tctccgttca atttactgat tgtcctcgac aggccttaag 1561 ggccagatct tgggcccggt acccaattcg ccctatagtg agtcgtatta caattcactg 1621 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 1681 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 1741 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa 1801 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 1861 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 1921 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 1981 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 2041 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 2101 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 2161 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 2221 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 2281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 2341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 2401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 2461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 3001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 3061 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 3121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 3181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 3241 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 3301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 3361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 3541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 4021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 4081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 4141 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4201 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 4261 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 4321 cggctcgtat gttgtgtgga attgtgagcg gataacaatt t //