LOCUS C335p7o-LH-UbiAt 10861 bp DNA circular SYN 29-SEP-2017 DEFINITION Ligation of 204pUbiAtmu-ocs* into B414p7o-LH* ACCESSION C335p7o-LH-UbiAt KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10861) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B414p7o-LH* Fragment ends: SfiI Fragment size: 9121 Insert molecule: 204pUbiAtmu-ocs* Fragment ends: SfiI Fragment size: 1740 Sequencing primers BarF CTTCTGGCAGCTCGACTTCT SphR GGGTGGTTGAACAGAAGTCAT Ubq10-F CTAGATCTGGTGTTAGTTTCTAGT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..978 /gene="Bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 979..1167 /gene="STLS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Int" /SECDescr="STLS1" CDS 1168..1483 /gene="Bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1496..1786 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1835..3310 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3005..3308 /gene="Int" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3358..3553 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 3638..3661 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4196..4893 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4930..5189 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5442..9147 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9331..10341 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt 781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc 841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg 901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc 961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat 1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat 1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat 1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc 1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac 1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc 1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg 1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc 1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt 1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt 1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat 1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt 1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca 1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca 1801 tgcaagctga tccactagag gccatggcgg ccgctttcct tacattctga gcctctttcc 1861 ttctaatcca ctcatctgca tcttcttgtg tccttactaa tacctcattg gttccaaatt 1921 ccctcccttt aagcaccagc tcgtttctgt tcttccacag cctcccaagt atccaaggga 1981 ctaaagcctc cacattcttc agatcaggat attcttgttt aagatgttga actctatgga 2041 ggtttgtatg aactgatgat ctaggaccgg ataagttccc ttcttcatag cgaacttatt 2101 caaagaatgt tttgtgtatc attcttgtta cattgttatt aatgaaaaaa tattattggt 2161 cattggactg aacacgagtg ttaaatatgg accaggcccc aaataagatc cattgatata 2221 tgaattaaat aacaagaata aatcgagtca ccaaaccact tgcctttttt aacgagactt 2281 gttcaccaac ttgatacaaa agtcattatc ctatgcaaat caataatcat acaaaaatat 2341 ccaataacac taaaaaatta aaagaaatgg ataatttcac aatatgttat acgataaaga 2401 agttactttt ccaagaaatt cactgatttt ataagcccac ttgcattaga taaatggcaa 2461 aaaaaaacaa aaaggaaaag aaataaagca cgaagaattc tagaaaatac gaaatacgct 2521 tcaatgcagt gggacccacg gttcaattat tgccaatttt cagctccacc gtatatttaa 2581 aaaataaaac gataatgcta aaaaaatata aatcgtaacg atcgttaaat ctcaacggct 2641 ggatcttatg acgaccgtta gaaattgtgg ttgtcgacga gtcagtaata aacggcgtca 2701 aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc 2761 ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata 2821 cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg 2881 tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac 2941 aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat 3001 caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc 3061 ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc 3121 tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga 3181 tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta 3241 gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg 3301 attaacaggt actagtggat cccccgggct gcaggaattc aagcttacgc gtgtcgaccc 3361 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg 3421 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca 3481 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat 3541 ttactgattg tcctcgacag gccttaaggg ccagatcttg ggcccggtac ccgatcagat 3601 tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa 3661 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt 3721 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg agtgcttggc 3781 attccgtgcg ataatgactt ctgttcaacc acccaaacgt cggaaagcct gacgacggag 3841 cagcattcca aaaagatccc ttggctcgtc tgggtcggct agaaggtcga gtgggctgct 3901 gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc 3961 aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc 4021 agtcatcacg cgccaaagtt ccgtcacagg atgatctggc gcgagttgct ggatctcgcc 4081 ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg 4141 gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgaaaag 4201 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4441 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 4921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 4981 tgtggataac cgattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5041 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 5101 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 5161 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 5221 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 5281 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 5341 caccgaaacg cgcgaggcag gggtacgtcg aggtcgatcc aacccctccg ctgctatagt 5401 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc 5461 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt 5521 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc 5581 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac 5641 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc 5701 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt 5761 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc 5821 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc 5881 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt 5941 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag 6001 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac 6061 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg 6121 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc 6181 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa 6241 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag 6301 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa 6361 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctcgcg gcctggccgg 6421 cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt 6481 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac 6541 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac 6601 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt 6661 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct 6721 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg 6781 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa 6841 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc 6901 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc 6961 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct 7021 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg 7081 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga 7141 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag 7201 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag 7261 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg 7321 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc 7381 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag 7441 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga 7501 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt 7561 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt 7621 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag 7681 ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa 7741 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt 7801 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg 7861 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg 7921 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta 7981 gaggtttccg cagggccggc cggcatggcg agtgtgtggg attacgacct ggtactgatg 8041 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc 8101 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc 8161 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc 8221 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc 8281 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc 8341 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg 8401 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca 8461 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt 8521 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat 8581 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg 8641 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg 8701 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc 8761 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca 8821 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc 8881 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt 8941 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg 9001 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa 9061 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc 9121 gaccgccggc gcccacatca aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa 9181 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt 9241 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt 9301 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg 9361 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt 9421 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga 9481 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag 9541 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt 9601 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca 9661 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg 9721 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct 9781 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt 9841 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt 9901 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa 9961 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt 10021 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc 10081 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca 10141 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg 10201 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat 10261 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc 10321 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg 10381 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg 10441 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata 10501 tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata 10561 gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta cctgcgttca aatactttgc 10621 gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca cggatcgact gcttctctcg 10681 caacgccatc cgacggatga tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg 10741 ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc aatggaaatt atctgcctaa 10801 ccggctcagt tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg 10861 c //