LOCUS C387pGusXM 4984 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of GusM-Hind-Sal* into pOCS-BM* ACCESSION C387pGusXM KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4984) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pOCS-BM* Fragment ends: SalI and HindIII Fragment size: 3146 Insert molecule: GusM-Hind-Sal* Fragment ends: HindIII and SalI Fragment size: 1838 Sequencing primers: M13RY CACACAGGAAACAGCTATGAC gc rich OR2 GTGCACAACAGAATTGAAAGCAAA gc rich FEATURES Location/Qualifiers CDS 182..1993 /gene="GusX" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1999..2194 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 3010..3870 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3913..4629 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggctgcagg aattcaagct tggcacgcgt cctccaccga 181 aatgctccgc cccgtggaga ccccaactcg tgagatcaag aagctggacg gcttgtgggc 241 cttctccctt gacagggaaa actgcggaat tgatcagaga tggtgggagt ccgctctcca 301 agagtctcgc gccatcgccg tccctggttc gttcaacgac cagttcgcag acgctgacat 361 ccgcaattac gccggcaacg tttggtatca gagggaagtc ttcatcccga aggggtgggc 421 cggacagcgc attgtcctgc gctttgatgc ggtgacccac tacggaaaag tctgggttaa 481 caaccaagaa gtgatggagc accagggcgg atacacaccc ttcgaagccg acgtcacccc 541 atacgtgatc gccggcaagt ccgtccgtat caccgtttgc gtgaacaacg agttgaattg 601 gcagaccatc ccaccaggca tggtgattac ggacgagaac ggtaagaaga agcaaagcta 661 tttccacgac ttcttcaact acgccggaat ccacaggagc gtgatgctct acaccactcc 721 taacacctgg gtggacgata tcaccgtcgt tacccatgtc gcccaggact gcaaccacgc 781 ctccgttgac tggcaggtgg tggccaatgg cgacgtttca gtcgagctta gagatgccga 841 ccagcaagtg gtcgcaactg gacagggaac ctccggcacc ctccaagtgg tcaacccgca 901 cctgtggcag cccggtgagg gctacctcta cgagttgtgc gttacagcta agagtcagac 961 cgagtgcgac atttacccac tgcgcgtggg aatccgttct gtcgccgtga agggcgagca 1021 gttcctcatc aaccacaagc ccttctactt cacggggttt ggccgccacg aggacgccga 1081 tcttagggga aagggcttcg acaacgtctt gatggttcac gaccacgctc tcatggactg 1141 gatcggtgcc aactcctacc gcaccagcca ctacccttac gccgaggaga tgctggactg 1201 ggcggatgaa cacggaattg tggtcatcga cgagaccgct gctgtgggct tcaacctctc 1261 gcttgggatc ggattcgagg ccggaaataa gcccaaggag ctgtactccg aggaggccgt 1321 caacggcgaa actcagcagg ctcacctcca ggccattaag gagttgatcg caagagacaa 1381 gaaccatcca agcgtggtta tgtggtccat cgccaacgag ccggacaccc gtccccaggg 1441 agctagggag tacttcgccc cactcgccga ggctacccgc aagctcgatc ccacccgccc 1501 tatcacttgc gtcaacgtga tgttctgcga cgcccacacc gacacaattt cagacctttt 1561 cgacgtcttg tgcctcaacc gttattacgg ctggtacgtg cagagcggtg atctggagac 1621 cgccgaaaag gttttggaga aggagctgct cgcgtggcaa gagaagctcc accagccgat 1681 catcatcacc gagtacgggg tggacacgct ggccggactc cactccatgt ataccgatat 1741 gtggtctgag gaataccagt gcgcttggct tgacatgtac cacagggtgt tcgaccgtgt 1801 gtcggccgtg gttggagagc aagtctggaa ttttgcagac ttcgccacca gccagggcat 1861 tttgagagtg ggaggcaaca agaaaggtat cttcactcgt gacaggaagc caaagtccgc 1921 tgccttcctg ctccagaagc gctggaccgg catgaacttc ggggagaagc cccagcaagg 1981 aggcaagcag taagtcgacc ctgctttaat gagatatgcg agacgcctat gatcgcatga 2041 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc 2101 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat 2161 gaataatatt ctccgttcaa tttactgatt gtcctcgaca ggccttaagg gccagatctt 2221 gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt 2281 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 2341 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 2401 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta 2461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 2521 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 2581 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 2641 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 2701 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 2761 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 2821 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca 2881 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 2941 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 3001 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 3061 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 3121 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 3181 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 3241 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 3301 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 3361 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 3421 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 3481 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 3541 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 3601 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 3661 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 3721 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 3781 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 3841 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 3901 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 3961 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 4021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 4081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 4141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 4201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 4261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 4321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 4501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 4561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 4741 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 4801 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4861 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4921 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 4981 ttgt //