LOCUS D029p9ioR-U10Cas 14739 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of B763p9ioR-35sD10Aoi* into C111p9ioR-Uq10CasWToi* ACCESSION D029p9ioR-U10Cas KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 14739) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: C111p9ioR-Uq10CasWToi* Fragment ends: AfeI and BamHI Fragment size: 13216 Insert molecule: B763p9ioR-35sD10Aoi* Fragment ends: BamHI and AfeI Fragment size: 1523 Sequencing primers Ubq10-F CTAGATCTGGTGTTAGTTTCTAGT CasF2 CCTTACTACGTCGGACCCTT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1043 /gene="NptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1044..1230 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 CDS 1231..1734 /gene="NptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1748..2038 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2087..3562 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3191..3254 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3257..3560 /product="íntron" /SECDrawAs="Region" /SECStyleId=1 CDS 3584..7786 /gene="D10A'" /translation="MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVP SKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIF SNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVD STDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAE DAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFI KPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLK DNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFI ERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLD NEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKL INGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHI ANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMK RIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDH IVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFD NLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVI TLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYK VYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEI VWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKK YGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYK EVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLK GSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDL SQLGGDKRPAATKKAGQAKKKK" /product="Cas9 nickase" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Cas9D10A" /SECDescr="Cas9 nickase" misc_feature 3590..3610 /product="NLS" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3660 /product="D10A mut to C" /SECDrawAs="Region" /SECStyleId=1 misc_feature 7736..7783 /product="NLS" /SECDrawAs="Region" /SECStyleId=1 misc_feature 7792..8045 /gene="NOS-T" /product="Nos-Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="NOS-T" /SECDescr="Nos-Terminator" misc_feature 8055..8499 /gene="U6At" /product="Promoter" /SECDrawAs="Region" /SECStyleId=1 CDS 8500..8526 /product="targetRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 8527..8556 /product="hairpin" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 8527..8609 /gene="gRNA" /product="gRNA" /SECDrawAs="Region" /SECStyleId=1 misc_feature 8695..8718 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 9253..9950 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 10238..13025 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 13209..14219 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct 781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg 1741 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 1801 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 1861 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 1921 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 1981 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga 2041 agcctgcagg catgcaagct gatccactag aggccatggc ggccgctttc cttacattct 2101 gagcctcttt ccttctaatc cactcatctg catcttcttg tgtccttact aatacctcat 2161 tggttccaaa ttccctccct ttaagcacca gctcgtttct gttcttccac agcctcccaa 2221 gtatccaagg gactaaagcc tccacattct tcagatcagg atattcttgt ttaagatgtt 2281 gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc ccttcttcat 2341 agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta ttaatgaaaa 2401 aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc ccaaataaga 2461 tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca cttgcctttt 2521 ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa atcaataatc 2581 atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc acaatatgtt 2641 atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc acttgcatta 2701 gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat tctagaaaat 2761 acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt ttcagctcca 2821 ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa cgatcgttaa 2881 atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac gagtcagtaa 2941 taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca 3001 caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa 3061 caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc 3121 gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct 3181 cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc tctcaattct 3241 ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct tcgattttgt 3301 tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa tcttagatcg 3361 aagacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta gggtttcata 3421 gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc ttcgatttgt 3481 gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg tcgattaatc 3541 tgagtttttc tgattaacag gtactagtgg atcctccata actatggccc ccaagaagaa 3601 gcgcaaagtg ggaatccatg gcgtcccagc cgcagacaag aagtacagca ttggtctggc 3661 catcgggacc aactccgtcg gctgggccgt tatcaccgat gagtacaaag tgccttctaa 3721 gaagttcaaa gtcctcggaa acactgacag gcacagcatc aagaagaact tgattggcgc 3781 ccttctcttc gactccggcg aaaccgctga ggccacccgt ctgaagcgca ccgccagaag 3841 gcgctacacc cgtcgcaaga ataggatctg ctacctccag gagatcttca gcaacgagat 3901 ggccaaagtg gacgattcct tctttcaccg tttggaggag agcttccttg tcgaggagga 3961 caagaagcac gagcgccatc ccatcttcgg aaacattgtt gacgaggtgg cttaccacga 4021 gaagtatcca actatctacc acctcagaaa gaagctggtg gactccaccg acaaggccga 4081 tttgaggctc atctaccttg ccctcgctca catgatcaag ttccgcggtc acttcctgat 4141 tgagggcgac ctcaaccccg acaactctga cgtcgataag ttgttcatcc aactggtcca 4201 gacctacaac cagcttttcg aggagaatcc tatcaacgcc tccggggttg acgccaaggc 4261 tattctcagc gcccgtctgt ccaagagccg caggcttgag aacttgatcg cccagctccc 4321 aggagagaag aagaacggcc tcttcggaaa cctgatcgct ctctccttgg gccttacccc 4381 taacttcaag tctaattttg acctcgccga ggacgccaag ctgcaactca gcaaggacac 4441 ctacgatgac gacttggaca acctgctcgc ccagatcggc gatcagtacg ctgacctttt 4501 cttggccgcc aagaacctct ccgacgctat tctgctctcc gacatccttc gcgtgaacac 4561 tgagatcacc aaggccccac tgagcgcctc catgatcaag cgttacgacg aacaccacca 4621 agatttgacc ctcctcaagg ctcttgtcag acagcagctg cccgagaagt acaaggagat 4681 tttcttcgac cagagcaaga acggatacgc cggttacatc gacggcgggg cctcccaaga 4741 ggagttctac aagttcatca agcccatctt ggagaagatg gacggcaccg aagagctgct 4801 cgtgaagctc aaccgcgagg atcttttgag gaagcagcgt accttcgaca acggatctat 4861 tccccaccag atccatctcg gcgagctgca cgctatcctc cgcaggcaag aggacttcta 4921 tccattcctt aaggacaatc gcgagaagat tgaaaagatc ctcactttta gaatccctta 4981 ctacgtcgga cccttggccc gtggcaacag ccgcttcgcc tggatgacca ggaagtccga 5041 ggaaaccatc accccatgga acttcgagga ggtggttgac aagggggcca gcgctcagtc 5101 cttcattgag cgcatgacca acttcgataa gaacctgccc aacgagaaag tcctcccaaa 5161 gcactccctt ctgtacgagt acttcactgt ctacaatgag ttgaccaaag tgaagtacgt 5221 gaccgagggt atgcgtaagc ctgccttcct cagcggcgag cagaagaagg ccatcgttga 5281 cctcctgttc aagaccaaca ggaaagtcac cgtgaagcag cttaaggagg actacttcaa 5341 gaagatcgag tgctttgact ctgtcgagat cagcggagtg gaggatcgct tcaacgcttc 5401 cttgggcact taccacgacc tcctgaagat tatcaaggac aaggacttcc tcgacaacga 5461 ggagaacgaa gatatcttgg aggacatcgt tcttaccctc accctgttcg aggacagaga 5521 gatgattgag gaacgcctca agacctacgc ccacttgttc gacgataaag tcatgaagca 5581 acttaagcgt aggcgctaca ccggatgggg caggctgtcc cgtaagctca tcaacggtat 5641 ccgcgacaag cagagcggca agactattct cgacttcttg aagtccgacg gattcgccaa 5701 tcgcaacttc atgcagctga tccacgacga ttctcttacc ttcaaggagg acatccaaaa 5761 ggctcaagtg tccggccagg gcgacagcct ccacgagcac atcgccaacc tggccgggag 5821 ccccgctatt aagaagggaa tcctccagac cgtcaaggtg gttgacgagt tggtcaaggt 5881 gatgggcaga cataagccag agaacatcgt catcgagatg gccagggaaa accagaccac 5941 ccagaaggga cagaagaact cccgtgagcg catgaagagg attgaggagg gcatcaagga 6001 gcttggttcc cagatcctca aggagcaccc cgtggaaaac acccaactcc agaatgagaa 6061 gctgtatttg tactaccttc agaacgggcg cgatatgtac gttgaccaag agctggacat 6121 caaccgcctc agcgactacg acgtggatca cattgtccct cagtcctttc tcaaggacga 6181 ctctatcgac aacaaggtgt tgactagaag cgataagaac cgcggcaagt ccgacaacgt 6241 cccatccgag gaggtggtca agaagatgaa gaattactgg aggcagctgc ttaacgccaa 6301 gctcatcacc cagcgcaagt tcgacaactt gaccaaggcc gagcgtggag gcctcagcga 6361 actggacaag gctggattca tcaagaggca acttgttgaa acccgccaga ttaccaagca 6421 cgtggcccag atcctcgact cccgtatgaa cactaagtac gatgagaacg acaagctgat 6481 ccgcgaggtc aaagtgatta ccctcaagag caagctcgtg tctgacttca gaaaggactt 6541 ccaattctac aaggttaggg agatcaacaa ctaccaccac gcccacgatg cttaccttaa 6601 tgccgtggtc ggcaccgcct tgatcaagaa gtaccccaag ctggagtccg agttcgtgta 6661 tggtgactac aaggtctacg acgttcgcaa gatgatcgct aagagcgagc aggagattgg 6721 caaggccacc gccaagtact tcttctactc caacatcatg aacttcttta agaccgagat 6781 cactttggct aacggggaga tccgtaagcg ccctctcatt gaaaccaacg gagaaaccgg 6841 cgagatcgtg tgggacaagg gcagggactt cgccaccgtc agaaaagtgc tgagcatgcc 6901 ccaagtcaac atcgtgaaga aaaccgaggt tcagactgga ggcttctcca aggagtctat 6961 cctcccaaag cgcaattccg ataagttgat tgcccgtaag aaggactggg accccaagaa 7021 gtacggtgga ttcgacagcc caaccgtcgc ctactccgtg cttgtcgtgg ctaaagttga 7081 gaagggcaag agcaagaagc tcaagtccgt caaggagctg ctcgggatca ccatcatgga 7141 gcgcagctcc ttcgagaaga accctattga tttccttgag gccaagggct acaaggaagt 7201 gaagaaggac ttgatcatca agctccccaa gtactctctg ttcgagcttg agaacggaag 7261 gaagcgtatg ctcgcctccg ctggcgagct gcaaaaggga aacgagttgg ccctcccaag 7321 caagtacgtc aacttcctgt acctcgcctc ccactatgag aagctcaagg gcagccccga 7381 ggacaacgaa cagaagcagt tgtttgtgga gcagcataag cactaccttg acgagatcat 7441 tgagcagatc agcgagttct ccaagcgcgt tatcctggct gacgccaatc tcgataaagt 7501 cctttctgcc tacaacaagc acagggacaa gcctatcaga gagcaggctg aaaacattat 7561 ccacttgttc accctcacca acctgggtgc cccagccgcc ttcaagtact tcgacactac 7621 catcgaccgc aagcgttaca cctccaccaa ggaagtgctc gatgctaccc ttatccacca 7681 gagcattact gggttgtacg aaaccaggat cgacctgtcc caactcggcg gagacaagcg 7741 ccccgccgcc accaagaagg ctggccaggc caagaagaag aagtaacccg ggatcgttca 7801 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc 7861 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta 7921 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa 7981 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta 8041 gatcgctcga attccttcgt tgaacaacgg aaactcgact tgccttccgc acaatacatc 8101 atttcttctt agcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct 8161 tacattttct tgaaccgtag ctttcgtttt cttcttttta actttccatt cggagttttt 8221 gtatcttgtt tcatagtttg tcccaggatt agaatgatta ggcatcgaac cttcaagaat 8281 ttgattgaat aaaacatctt cattcttaag atatgaagat aatcttcaaa aggcccctgg 8341 gaatctgaaa gaagagaagc aggcccattt atatgggaaa gaacaatagt atttcttata 8401 taggcccatt taagttgaaa acaatcttca aaagtcccac atcgcttaga taagaaaacg 8461 aagctgagtt tatatacagc tagagtcgaa gtagtgattg agacgttcga agcttctgtc 8521 gtctcagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga 8581 aaaagtggca ccgagtcggt gctttttttg tcgacaggcc ttaagggcca gatcttgggc 8641 ccggtacccg atcagattgt cgtttcccgc cttcggttta aactatcagt gtttgacagg 8701 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa 8761 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc 8821 cctcgggagt gcttggcatt ccgtgcgata atgacttctg ttcaaccacc caaacgtcgg 8881 aaagcctgac gacggagcag cattccaaaa agatcccttg gctcgtctgg gtcggctaga 8941 aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt cgcggacgta gcgcagcgcc 9001 gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg tgatctgctt gtcgctcttt 9061 cggccgacgt cctggccagt catcacgcgc caaagttccg tcacaggatg atctggcgcg 9121 agttgctgga tctcgccttc aatccgggtc tgtggcggga actccacgaa aatatccgaa 9181 cgcagcaaga tatcgcggtg catctcggtc ttgcctgggc agtcgccgcc gacgccgttg 9241 atgtggacgc cgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 9301 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 9361 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 9421 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 9481 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 9541 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 9601 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 9661 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 9721 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 9781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 9841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 9901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 9961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 10021 gcgttatccc ctgattctgt ggataaccga ttaccgcctt tgagtgagct gataccgctc 10081 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga 10141 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 10201 gtacaatctg ctctgatgcc gcatagttaa gccagtaacc gtgcggctgc atgaaatcct 10261 ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac 10321 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt 10381 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt 10441 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc 10501 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt 10561 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc 10621 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga 10681 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt 10741 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag 10801 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc 10861 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt 10921 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc 10981 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa 11041 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca 11101 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg 11161 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc 11221 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg 11281 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa 11341 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt 11401 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc 11461 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac 11521 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc 11581 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc 11641 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc 11701 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg 11761 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg 11821 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg 11881 gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat 11941 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg 12001 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg 12061 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca 12121 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg 12181 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc 12241 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga 12301 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag 12361 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga 12421 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga 12481 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg 12541 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag 12601 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc 12661 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt 12721 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt 12781 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca 12841 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg 12901 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca 12961 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag 13021 gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc 13081 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga 13141 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca 13201 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg 13261 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc 13321 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca 13381 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg 13441 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg 13501 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac 13561 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag 13621 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg 13681 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc 13741 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag 13801 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct 13861 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg 13921 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa 13981 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga 14041 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac 14101 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc 14161 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat 14221 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat 14281 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt 14341 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt 14401 cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg 14461 aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg 14521 gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg 14581 tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt 14641 gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa 14701 ccaacatgca agctccaccg ggtgcaaagc ggcagcggc //