LOCUS D023p7D35s-C9wt- 12916 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of C035p7ioR-Uq10CasWToi* into D011p7D35s* ACCESSION D023p7D35s-C9wt- KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 12916) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: D011p7D35s* Fragment ends: SfiI Fragment size: 6377 Insert molecule: C035p7ioR-Uq10CasWToi* Fragment ends: SfiI Fragment size: 6539 Sequencing primers: BarF CTTCTGGCAGCTCGACTTCT SphR GGGTGGTTGAACAGAAGTCAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB'" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 /SECName="LB" /SECDescr="Left Border" misc_feature 169..584 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 605..838 /gene="Bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 839..1027 /gene="STLS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 /SECName="STLS1" /SECDescr="Intron" CDS 1028..1343 /gene="Bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1356..1646 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1672..3147 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2776..2839 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2842..3145 /product="íntron" /SECDrawAs="Region" /SECStyleId=1 CDS 3169..7365 /gene="'Cas9WT" /translation="MAPKKKRKVGIHGAPDKKYSIGLDIGTNSVGWAVITDEYKVPSK KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ LGGDKRPAATKKAGQAKKKK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Cas9WToi" /SECDescr="no intronsequence" misc_feature 3175..3195 /product="NLS" /SECDrawAs="Region" /SECStyleId=1 misc_feature 7315..7362 /product="NLS" /SECDrawAs="Region" /SECStyleId=1 misc_feature 7371..7624 /gene="NOS-T" /product="Nos-Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="TNOS" /SECDescr="Nos-Terminator" misc_feature 7634..8078 /gene="U6At" /product="U6At" /SECDrawAs="Region" /SECStyleId=1 CDS 8079..8105 /product="targetRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 8106..8135 /product="hairpin" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 8106..8188 /gene="gRNA" /product="gRNA - scaffold" /SECDrawAs="Region" /SECStyleId=1 misc_feature 8234..8836 /product="Right Border Region" /SECDrawAs="Region" /SECStyleId=1 misc_feature 8279..8302 /gene="RB" /product="RB" /SECDrawAs="Region" /SECStyleId=1 /SECName="RB" /SECDescr="RB" misc_feature 8850..9508 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 misc_feature 9537..11500 /gene="''pVS1 ORI'''" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="pVS1" /SECDescr="pVS1 ORI" CDS 9863..11038 /gene="ParB" /SECDrawAs="Gene" /SECStyleId=1 /SECName="ParB" CDS 11689..12699 /gene="strep/spec" /product="Strep/Spec resistence" /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV GK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Sm/Sp" /SECDescr="Strep/Spec resistence" misc_feature join(12767..12916,1..163) /product="Left Border Region" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc 181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat 601 aactatgggc cccgagcgca ggcccgccga catccgccgt gccaccgagg ccgacatgcc 661 agccgtctgc accatcgtga accactacat cgagacttcc acggtcaact tccgcacaga 721 gccgcaggag cctcaggagt ggaccgacga cctcgtgagg ctgcgcgagc gctacccctg 781 gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggt 841 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata 901 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta 961 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga 1021 tgtgcaggca cgcaacgcct acgactggac ggcggagagc accgtctacg tgtccccacg 1081 ccaccagcgc accggcctgg gctccacgct ctacacccac ctgctcaaga gcctggaggc 1141 ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc aacgacccga gcgtccgtat 1201 gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg gccgcgggct tcaagcacgg 1261 caactggcac gacgtgggct tctggcagct cgacttctcc ctgcctgtcc caccccgccc 1321 ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt atggcattgg gaaaactgtt 1381 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc 1441 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat 1501 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata 1561 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc 1621 gaagttaata tgaggagtaa aacactgaag cctgcaggcc ttggcggccg ctttccttac 1681 attctgagcc tctttccttc taatccactc atctgcatct tcttgtgtcc ttactaatac 1741 ctcattggtt ccaaattccc tccctttaag caccagctcg tttctgttct tccacagcct 1801 cccaagtatc caagggacta aagcctccac attcttcaga tcaggatatt cttgtttaag 1861 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc 1921 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat 1981 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa 2041 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc 2101 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa 2161 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat 2221 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg 2281 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag 2341 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag 2401 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc 2461 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc 2521 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca 2581 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt 2641 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct 2701 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa 2761 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca 2821 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat 2881 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta 2941 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt 3001 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga 3061 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat 3121 taatctgagt ttttctgatt aacaggtact agtggatcct ccataactat ggcccccaag 3181 aagaagcgca aagtgggaat ccacggagct ccagacaaga agtacagcat tggtctggac 3241 atcgggacca actccgtcgg ctgggccgtt atcaccgatg agtacaaagt gccttctaag 3301 aagttcaaag tcctcggaaa cactgacagg cacagcatca agaagaactt gattggcgcc 3361 cttctcttcg actccggcga aaccgctgag gccacccgtc tgaagcgcac cgccagaagg 3421 cgctacaccc gtcgcaagaa taggatctgc tacctccagg agatcttcag caacgagatg 3481 gccaaagtgg acgattcctt ctttcaccgt ttggaggaga gcttccttgt cgaggaggac 3541 aagaagcacg agcgccatcc catcttcgga aacattgttg acgaggtggc ttaccacgag 3601 aagtatccaa ctatctacca cctcagaaag aagctggtgg actccaccga caaggccgat 3661 ttgaggctca tctaccttgc cctcgctcac atgatcaagt tccgcggtca cttcctgatt 3721 gagggcgacc tcaaccccga caactctgac gtcgataagt tgttcatcca actggtccag 3781 acctacaacc agcttttcga ggagaatcct atcaacgcct ccggggttga cgccaaggct 3841 attctcagcg cccgtctgtc caagagccgc aggcttgaga acttgatcgc ccagctccca 3901 ggagagaaga agaacggcct cttcggaaac ctgatcgctc tctccttggg ccttacccct 3961 aacttcaagt ctaattttga cctcgccgag gacgccaagc tgcaactcag caaggacacc 4021 tacgatgacg acttggacaa cctgctcgcc cagatcggcg atcagtacgc tgaccttttc 4081 ttggccgcca agaacctctc cgacgctatt ctgctctccg acatccttcg cgtgaacact 4141 gagatcacca aggccccact gagcgcctcc atgatcaagc gttacgacga acaccaccaa 4201 gatttgaccc tcctcaaggc tcttgtcaga cagcagctgc ccgagaagta caaggagatt 4261 ttcttcgacc agagcaagaa cggatacgcc ggttacatcg acggcggggc ctcccaagag 4321 gagttctaca agttcatcaa gcccatcttg gagaagatgg acggcaccga agagctgctc 4381 gtgaagctca accgcgagga tcttttgagg aagcagcgta ccttcgacaa cggatctatt 4441 ccccaccaga tccatctcgg cgagctgcac gctatcctcc gcaggcaaga ggacttctat 4501 ccattcctta aggacaatcg cgagaagatt gaaaagatcc tcacttttag aatcccttac 4561 tacgtcggac ccttggcccg tggcaacagc cgcttcgcct ggatgaccag gaagtccgag 4621 gaaaccatca ccccatggaa cttcgaggag gtggttgaca agggggccag cgctcagtcc 4681 ttcattgagc gcatgaccaa cttcgataag aacctgccca acgagaaagt cctcccaaag 4741 cactcccttc tgtacgagta cttcactgtc tacaatgagt tgaccaaagt gaagtacgtg 4801 accgagggta tgcgtaagcc tgccttcctc agcggcgagc agaagaaggc catcgttgac 4861 ctcctgttca agaccaacag gaaagtcacc gtgaagcagc ttaaggagga ctacttcaag 4921 aagatcgagt gctttgactc tgtcgagatc agcggagtgg aggatcgctt caacgcttcc 4981 ttgggcactt accacgacct cctgaagatt atcaaggaca aggacttcct cgacaacgag 5041 gagaacgaag atatcttgga ggacatcgtt cttaccctca ccctgttcga ggacagagag 5101 atgattgagg aacgcctcaa gacctacgcc cacttgttcg acgataaagt catgaagcaa 5161 cttaagcgta ggcgctacac cggatggggc aggctgtccc gtaagctcat caacggtatc 5221 cgcgacaagc agagcggcaa gactattctc gacttcttga agtccgacgg attcgccaat 5281 cgcaacttca tgcagctgat ccacgacgat tctcttacct tcaaggagga catccaaaag 5341 gctcaagtgt ccggccaggg cgacagcctc cacgagcaca tcgccaacct ggccgggagc 5401 cccgctatta agaagggaat cctccagacc gtcaaggtgg ttgacgagtt ggtcaaggtg 5461 atgggcagac ataagccaga gaacatcgtc atcgagatgg ccagggaaaa ccagaccacc 5521 cagaagggac agaagaactc ccgtgagcgc atgaagagga ttgaggaggg catcaaggag 5581 cttggttccc agatcctcaa ggagcacccc gtggaaaaca cccaactcca gaatgagaag 5641 ctgtatttgt actaccttca gaacgggcgc gatatgtacg ttgaccaaga gctggacatc 5701 aaccgcctca gcgactacga cgtggatcac attgtccctc agtcctttct caaggacgac 5761 tctatcgaca acaaggtgtt gactagaagc gataagaacc gcggcaagtc cgacaacgtc 5821 ccatccgagg aggtggtcaa gaagatgaag aattactgga ggcagctgct taacgccaag 5881 ctcatcaccc agcgcaagtt cgacaacttg accaaggccg agcgtggagg cctcagcgaa 5941 ctggacaagg ctggattcat caagaggcaa cttgttgaaa cccgccagat taccaagcac 6001 gtggcccaga tcctcgactc ccgtatgaac actaagtacg atgagaacga caagctgatc 6061 cgcgaggtca aagtgattac cctcaagagc aagctcgtgt ctgacttcag aaaggacttc 6121 caattctaca aggttaggga gatcaacaac taccaccacg cccacgatgc ttaccttaat 6181 gccgtggtcg gcaccgcctt gatcaagaag taccccaagc tggagtccga gttcgtgtat 6241 ggtgactaca aggtctacga cgttcgcaag atgatcgcta agagcgagca ggagattggc 6301 aaggccaccg ccaagtactt cttctactcc aacatcatga acttctttaa gaccgagatc 6361 actttggcta acggggagat ccgtaagcgc cctctcattg aaaccaacgg agaaaccggc 6421 gagatcgtgt gggacaaggg cagggacttc gccaccgtca gaaaagtgct gagcatgccc 6481 caagtcaaca tcgtgaagaa aaccgaggtt cagactggag gcttctccaa ggagtctatc 6541 ctcccaaagc gcaattccga taagttgatt gcccgtaaga aggactggga ccccaagaag 6601 tacggtggat tcgacagccc aaccgtcgcc tactccgtgc ttgtcgtggc taaagttgag 6661 aagggcaaga gcaagaagct caagtccgtc aaggagctgc tcgggatcac catcatggag 6721 cgcagctcct tcgagaagaa ccctattgat ttccttgagg ccaagggcta caaggaagtg 6781 aagaaggact tgatcatcaa gctccccaag tactctctgt tcgagcttga gaacggaagg 6841 aagcgtatgc tcgcctccgc tggcgagctg caaaagggaa acgagttggc cctcccaagc 6901 aagtacgtca acttcctgta cctcgcctcc cactatgaga agctcaaggg cagccccgag 6961 gacaacgaac agaagcagtt gtttgtggag cagcataagc actaccttga cgagatcatt 7021 gagcagatca gcgagttctc caagcgcgtt atcctggctg acgccaatct cgataaagtc 7081 ctttctgcct acaacaagca cagggacaag cctatcagag agcaggctga aaacattatc 7141 cacttgttca ccctcaccaa cctgggtgcc ccagccgcct tcaagtactt cgacactacc 7201 atcgaccgca agcgttacac ctccaccaag gaagtgctcg atgctaccct tatccaccag 7261 agcattactg ggttgtacga aaccaggatc gacctgtccc aactcggcgg agacaagcgc 7321 cccgccgcca ccaagaaggc tggccaggcc aagaagaaga agtaacccgg gatcgttcaa 7381 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca 7441 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat 7501 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa 7561 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag 7621 atcgctcgaa ttccttcgtt gaacaacgga aactcgactt gccttccgca caatacatca 7681 tttcttctta gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt 7741 acattttctt gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg 7801 tatcttgttt catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt 7861 tgattgaata aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg 7921 aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat 7981 aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga 8041 agctgagttt atatacagct agagtcgaag tagtgattga gacgttcgaa gcttctgtcg 8101 tctcagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa 8161 aaagtggcac cgagtcggtg ctttttttgt cgacaggcct taacggccag atctgctggg 8221 ccctgtggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga 8281 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt 8341 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg 8401 ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg 8461 tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc 8521 tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag 8581 cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct 8641 ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg 8701 cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc 8761 cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc 8821 gttgatgtgg acgccggctt agctcatggc caaaatccct taacgtgagt tttcgttcca 8881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 8941 cgtaatctgc tgcttgcaaa caaaaaaacc atcgctacca gcggtggttt gtttgccgga 9001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 9061 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 9121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 9181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 9241 ggggggttcg tgctcacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 9301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 9361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 9421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 9481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttc ctaagggtta 9541 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggtc gtccgagcgc 9601 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt 9661 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg 9721 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa 9781 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac 9841 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg 9901 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat 9961 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 10021 cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg aggcagaagc 10081 acgccccggt gaatcgtggc aagccgccgc tgatcgaatc cgcaaagaat cccggcaacc 10141 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 10201 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 10261 agtattccgc ctctcgaaac gtgatcgccg ggcgggcgaa gtcatccgct acgaactgcc 10321 ggacggtcat gtcgaggtga gcgcaggccc ggcgggcatg gcctccgtct gggattatga 10381 cctcgttctg atggccgtgt cgcaccttac ggaaagcatg aatcgctacc gggagggcaa 10441 gggtgataag cccggtcgcg tattccgtcc gcatgtggcc gacgttctga aattctgtcg 10501 ccgggcggat ggcggcaagc aaaaggacga tctcgtcgaa acctgcatcc gcctgaacac 10561 gacccacgtc gccatgcagc gcaccaagaa agcgaagaat ggccgtcttg tgactgtctc 10621 cgagggcgaa gccctgatct cgcgctacaa gatcgtaaag agcgaaaccg ggcggcccga 10681 gtacatcgag atcgagcttg ctgattggat gtaccgcgag atcacagaag gcaagaaccc 10741 ggacgtgctg acggttcacc ccgattactt tttgattgat cccggcatcg gtcgttttct 10801 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 10861 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 10921 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 10981 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 11041 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aagctctctt 11101 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 11161 cattgggaac ccaaagccgt acattgggaa ccgggcacgc atgtaagtga ctgatataaa 11221 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 11281 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaatagctgc aaaaagcgcc 11341 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgtggccgc 11401 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 11461 gccgtcgcca ctcgaccgcc gccgcccaca tcaaggcacc tcagcggtgg gtatgcctga 11521 cgatgcgtgc agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact 11581 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc 11641 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc 11701 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg 11761 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg 11821 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc 11881 agtcgcccta aaacaaagtt aaacatcatg cgtgaagcgg tgatcgccga agtatcgact 11941 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta 12001 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg 12061 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg 12121 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt 12181 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa 12241 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg 12301 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag 12361 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg 12421 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc 12481 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg 12541 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat 12601 cttggacaag aagaagatcg cttggcctct cgcgcagatc agttggaaga atttgtccac 12661 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc 12721 cgacgccgct tcgcggcgcg gcttaactca agcgttagac ggaccgccca tgtggatcac 12781 tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg 12841 aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt 12901 gcaaagcggc agcggc //