LOCUS B764p9ioR-35sCas 13681 bp DNA circular SYN 11-SEP-2017 DEFINITION Ligation of Cas9WToi Bam, Nru* into B357p9ioR-35sCasWT* ACCESSION B764p9ioR-35sCas KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 13681) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B357p9ioR-35sCasWT* Fragment ends: NruI and BamHI Fragment size: 12315 Insert molecule: Cas9WToi Bam, Nru* Fragment ends: BamHI and NruI Fragment size: 1366 Sequencing primers: CasNru CCTCGGACTTCCTGGTCATC E9F GTGTCAAATCGTGGCCTCTAA AatII-BamHI-2 GGATGAACAACTTATCGACGTCAGAGTTG Projekt Lukas FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 741..777 /gene="NptII" /product="Kanamycin resistance" /SECDrawAs="Gene" /SECStyleId=1 /SECName="NptII" /SECDescr="Kanamycin resistance" misc_feature 778..966 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 967..1733 /gene="NptII" /product="Kanamycin-Resistenz" /SECDrawAs="Gene" /SECStyleId=1 /SECName="NptII" /SECDescr="Kanamycin-Resistenz" misc_feature 1744..2034 /gene="'E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2089..2506 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 CDS 2477..2483 /product="TATA-box" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2538..2558 /gene="NLS" /SECDrawAs="Region" /SECStyleId=1 CDS 2538..6728 /gene="'Cas9WT" /translation="PKKKRKVGIHGAPDKKYSIGLDIGTNSVGWAVITDEYKVPSKKF KVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEM AKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDK ADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGV DAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKL QLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMI KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPIL EKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNRE KIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMT NFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFK TNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEEN EDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGI RDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLA GSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEE GIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQ SFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKS KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDV RKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDK GRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGF DSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKK DLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAEN IIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG GDKRPAATKKAGQAKKKK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Cas9WToi" /SECDescr="no intronsequence" misc_feature 2949..2956 /gene="int'" /product="intron from rbcs Nicotiana tobaccum (removed)" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6678..6725 /gene="NLS" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6734..6987 /gene="NOS-T" /product="Nos-Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="NOS-T" /SECDescr="Nos-Terminator" misc_feature 6992..7552 /gene="'RNA'" /SECDrawAs="Region" /SECStyleId=1 CDS 6997..7441 /gene="U6At" /SECDrawAs="Gene" /SECStyleId=1 CDS 7442..7468 /gene="targetRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 7469..7498 /gene="hairpin" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 7469..7551 /gene="gRNA" /product="scaffold" /SECDrawAs="Region" /SECStyleId=1 misc_feature 7637..7660 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 8195..8892 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 9180..11967 /gene="'pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 12151..13161 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtggccacc atgggcgcct ggattgaaca agatggattg cacgcaggta 781 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa 841 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag 901 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat 961 gtgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 1021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 1081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 1141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 1201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 1261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 1321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 1381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 1441 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 1501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 1561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 1621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 1681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtt 1741 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta 1801 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt 1861 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta 1921 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa 1981 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc 2041 tgcaggcatg caagctgatc cactagaggc catggcggcc gcactagatc aacatggtgg 2101 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg 2161 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag 2221 ctatctgtca cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc 2281 attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg 2341 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc 2401 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt 2461 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca ctagttggat 2521 cctccataac tatggccccc aagaagaagc gcaaagtggg aatccacgga gctccagaca 2581 agaagtacag cattggtctg gacatcggga ccaactccgt cggctgggcc gttatcaccg 2641 atgagtacaa agtgccttct aagaagttca aagtcctcgg aaacactgac aggcacagca 2701 tcaagaagaa cttgattggc gcccttctct tcgactccgg cgaaaccgct gaggccaccc 2761 gtctgaagcg caccgccaga aggcgctaca cccgtcgcaa gaataggatc tgctacctcc 2821 aggagatctt cagcaacgag atggccaaag tggacgattc cttctttcac cgtttggagg 2881 agagcttcct tgtcgaggag gacaagaagc acgagcgcca tcccatcttc ggaaacattg 2941 ttgacgaggt ggcttaccac gagaagtatc caactatcta ccacctcaga aagaagctgg 3001 tggactccac cgacaaggcc gatttgaggc tcatctacct tgccctcgct cacatgatca 3061 agttccgcgg tcacttcctg attgagggcg acctcaaccc cgacaactct gacgtcgata 3121 agttgttcat ccaactggtc cagacctaca accagctttt cgaggagaat cctatcaacg 3181 cctccggggt tgacgccaag gctattctca gcgcccgtct gtccaagagc cgcaggcttg 3241 agaacttgat cgcccagctc ccaggagaga agaagaacgg cctcttcgga aacctgatcg 3301 ctctctcctt gggccttacc cctaacttca agtctaattt tgacctcgcc gaggacgcca 3361 agctgcaact cagcaaggac acctacgatg acgacttgga caacctgctc gcccagatcg 3421 gcgatcagta cgctgacctt ttcttggccg ccaagaacct ctccgacgct attctgctct 3481 ccgacatcct tcgcgtgaac actgagatca ccaaggcccc actgagcgcc tccatgatca 3541 agcgttacga cgaacaccac caagatttga ccctcctcaa ggctcttgtc agacagcagc 3601 tgcccgagaa gtacaaggag attttcttcg accagagcaa gaacggatac gccggttaca 3661 tcgacggcgg ggcctcccaa gaggagttct acaagttcat caagcccatc ttggagaaga 3721 tggacggcac cgaagagctg ctcgtgaagc tcaaccgcga ggatcttttg aggaagcagc 3781 gtaccttcga caacggatct attccccacc agatccatct cggcgagctg cacgctatcc 3841 tccgcaggca agaggacttc tatccattcc ttaaggacaa tcgcgagaag attgaaaaga 3901 tcctcacttt tagaatccct tactacgtcg gacccttggc ccgtggcaac agccgcttcg 3961 cctggatgac caggaagtcc gaggaaacca tcaccccatg gaacttcgag gaggtggttg 4021 acaagggggc cagcgctcag tccttcattg agcgcatgac caacttcgat aagaacctgc 4081 ccaacgagaa agtcctccca aagcactccc ttctgtacga gtacttcact gtctacaatg 4141 agttgaccaa agtgaagtac gtgaccgagg gtatgcgtaa gcctgccttc ctcagcggcg 4201 agcagaagaa ggccatcgtt gacctcctgt tcaagaccaa caggaaagtc accgtgaagc 4261 agcttaagga ggactacttc aagaagatcg agtgctttga ctctgtcgag atcagcggag 4321 tggaggatcg cttcaacgct tccttgggca cttaccacga cctcctgaag attatcaagg 4381 acaaggactt cctcgacaac gaggagaacg aagatatctt ggaggacatc gttcttaccc 4441 tcaccctgtt cgaggacaga gagatgattg aggaacgcct caagacctac gcccacttgt 4501 tcgacgataa agtcatgaag caacttaagc gtaggcgcta caccggatgg ggcaggctgt 4561 cccgtaagct catcaacggt atccgcgaca agcagagcgg caagactatt ctcgacttct 4621 tgaagtccga cggattcgcc aatcgcaact tcatgcagct gatccacgac gattctctta 4681 ccttcaagga ggacatccaa aaggctcaag tgtccggcca gggcgacagc ctccacgagc 4741 acatcgccaa cctggccggg agccccgcta ttaagaaggg aatcctccag accgtcaagg 4801 tggttgacga gttggtcaag gtgatgggca gacataagcc agagaacatc gtcatcgaga 4861 tggccaggga aaaccagacc acccagaagg gacagaagaa ctcccgtgag cgcatgaaga 4921 ggattgagga gggcatcaag gagcttggtt cccagatcct caaggagcac cccgtggaaa 4981 acacccaact ccagaatgag aagctgtatt tgtactacct tcagaacggg cgcgatatgt 5041 acgttgacca agagctggac atcaaccgcc tcagcgacta cgacgtggat cacattgtcc 5101 ctcagtcctt tctcaaggac gactctatcg acaacaaggt gttgactaga agcgataaga 5161 accgcggcaa gtccgacaac gtcccatccg aggaggtggt caagaagatg aagaattact 5221 ggaggcagct gcttaacgcc aagctcatca cccagcgcaa gttcgacaac ttgaccaagg 5281 ccgagcgtgg aggcctcagc gaactggaca aggctggatt catcaagagg caacttgttg 5341 aaacccgcca gattaccaag cacgtggccc agatcctcga ctcccgtatg aacactaagt 5401 acgatgagaa cgacaagctg atccgcgagg tcaaagtgat taccctcaag agcaagctcg 5461 tgtctgactt cagaaaggac ttccaattct acaaggttag ggagatcaac aactaccacc 5521 acgcccacga tgcttacctt aatgccgtgg tcggcaccgc cttgatcaag aagtacccca 5581 agctggagtc cgagttcgtg tatggtgact acaaggtcta cgacgttcgc aagatgatcg 5641 ctaagagcga gcaggagatt ggcaaggcca ccgccaagta cttcttctac tccaacatca 5701 tgaacttctt taagaccgag atcactttgg ctaacgggga gatccgtaag cgccctctca 5761 ttgaaaccaa cggagaaacc ggcgagatcg tgtgggacaa gggcagggac ttcgccaccg 5821 tcagaaaagt gctgagcatg ccccaagtca acatcgtgaa gaaaaccgag gttcagactg 5881 gaggcttctc caaggagtct atcctcccaa agcgcaattc cgataagttg attgcccgta 5941 agaaggactg ggaccccaag aagtacggtg gattcgacag cccaaccgtc gcctactccg 6001 tgcttgtcgt ggctaaagtt gagaagggca agagcaagaa gctcaagtcc gtcaaggagc 6061 tgctcgggat caccatcatg gagcgcagct ccttcgagaa gaaccctatt gatttccttg 6121 aggccaaggg ctacaaggaa gtgaagaagg acttgatcat caagctcccc aagtactctc 6181 tgttcgagct tgagaacgga aggaagcgta tgctcgcctc cgctggcgag ctgcaaaagg 6241 gaaacgagtt ggccctccca agcaagtacg tcaacttcct gtacctcgcc tcccactatg 6301 agaagctcaa gggcagcccc gaggacaacg aacagaagca gttgtttgtg gagcagcata 6361 agcactacct tgacgagatc attgagcaga tcagcgagtt ctccaagcgc gttatcctgg 6421 ctgacgccaa tctcgataaa gtcctttctg cctacaacaa gcacagggac aagcctatca 6481 gagagcaggc tgaaaacatt atccacttgt tcaccctcac caacctgggt gccccagccg 6541 ccttcaagta cttcgacact accatcgacc gcaagcgtta cacctccacc aaggaagtgc 6601 tcgatgctac ccttatccac cagagcatta ctgggttgta cgaaaccagg atcgacctgt 6661 cccaactcgg cggagacaag cgccccgccg ccaccaagaa ggctggccag gccaagaaga 6721 agaagtaacc cgggatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt 6781 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat 6841 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt 6901 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg 6961 cgcggtgtca tctatgttac tagatcgctc gaattccttc gttgaacaac ggaaactcga 7021 cttgccttcc gcacaataca tcatttcttc ttagcttttt ttcttcttct tcgttcatac 7081 agtttttttt tgtttatcag cttacatttt cttgaaccgt agctttcgtt ttcttctttt 7141 taactttcca ttcggagttt ttgtatcttg tttcatagtt tgtcccagga ttagaatgat 7201 taggcatcga accttcaaga atttgattga ataaaacatc ttcattctta agatatgaag 7261 ataatcttca aaaggcccct gggaatctga aagaagagaa gcaggcccat ttatatggga 7321 aagaacaata gtatttctta tataggccca tttaagttga aaacaatctt caaaagtccc 7381 acatcgctta gataagaaaa cgaagctgag tttatataca gctagagtcg aagtagtgat 7441 tgagacgttc gaagcttctg tcgtctcagt tttagagcta gaaatagcaa gttaaaataa 7501 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tgtcgacagg 7561 ccttaagggc cagatcttgg gcccggtacc cgatcagatt gtcgtttccc gccttcggtt 7621 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta 7681 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 7741 tgcatgccaa ccacagggtt cccctcggga gtgcttggca ttccgtgcga taatgacttc 7801 tgttcaacca cccaaacgtc ggaaagcctg acgacggagc agcattccaa aaagatccct 7861 tggctcgtct gggtcggcta gaaggtcgag tgggctgctg tggcttgatc cctcaacgcg 7921 gtcgcggacg tagcgcagcg ccgaaaaatc ctcgatcgca aatccgacgc tgtcgaaaag 7981 cgtgatctgc ttgtcgctct ttcggccgac gtcctggcca gtcatcacgc gccaaagttc 8041 cgtcacagga tgatctggcg cgagttgctg gatctcgcct tcaatccggg tctgtggcgg 8101 gaactccacg aaaatatccg aacgcagcaa gatatcgcgg tgcatctcgg tcttgcctgg 8161 gcagtcgccg ccgacgccgt tgatgtggac gccgaaaagg atctaggtga agatcctttt 8221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 8281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 8341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 8401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 8461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 8521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 8581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 8641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 8701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 8761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 8821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 8881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 8941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gattaccgcc 9001 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 9061 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 9121 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtaa 9181 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctcgcg gcctggccgg 9241 cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt 9301 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac 9361 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac 9421 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt 9481 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct 9541 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg 9601 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa 9661 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc 9721 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc 9781 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct 9841 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg 9901 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga 9961 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag 10021 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag 10081 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg 10141 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc 10201 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag 10261 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga 10321 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt 10381 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt 10441 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag 10501 ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa 10561 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt 10621 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg 10681 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg 10741 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta 10801 gaggtttccg cagggccggc cggcatggcg agtgtgtggg attacgacct ggtactgatg 10861 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc 10921 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc 10981 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc 11041 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc 11101 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc 11161 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg 11221 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca 11281 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt 11341 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat 11401 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg 11461 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg 11521 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc 11581 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca 11641 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc 11701 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt 11761 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg 11821 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa 11881 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc 11941 gaccgccggc gcccacatca aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa 12001 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt 12061 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt 12121 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg 12181 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt 12241 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga 12301 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag 12361 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt 12421 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca 12481 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg 12541 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct 12601 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt 12661 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt 12721 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa 12781 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt 12841 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc 12901 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca 12961 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg 13021 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat 13081 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc 13141 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg 13201 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg 13261 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata 13321 tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata 13381 gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta cctgcgttca aatactttgc 13441 gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca cggatcgact gcttctctcg 13501 caacgccatc cgacggatga tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg 13561 ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc aatggaaatt atctgcctaa 13621 ccggctcagt tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg 13681 c //