LOCUS C232p7ioRAct-UZm 15535 bp DNA circular SYN 09-NOV-2016 DEFINITION Ligation of B489p7oAct-LH* into C143p6ioRAct-UZmCwtoi* ACCESSION C232p7ioRAct-UZm KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 15535) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: C143p6ioRAct-UZmCwtoi* Fragment ends: SbfI and BamHI Fragment size: 14145 Insert molecule: B489p7oAct-LH* Fragment ends: BamHI and SbfI Fragment size: 1390 Sequencing primers: BarF CTTCTGGCAGCTCGACTTCT baroR GGTCTCGATGTAGTGGTTCA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 313..1710 /gene="pAct'" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1738..1971 /gene="Bar'" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1972..2160 /gene="STLS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 /SECName="STLS1" /SECDescr="Intron" CDS 2161..2478 /gene="''Bar'" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2498..2788 /gene="'E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2843..4351 /gene="Ubi-int" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3830..4349 /gene="Intron" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4382..4402 /gene="NLS" /SECDrawAs="Region" /SECStyleId=1 CDS 4418..8572 /gene="'Cas9WT" /translation="PDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAG QAKKKK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="'Cas9WToi" /SECDescr="no intronsequence" misc_feature 8522..8569 /gene="NLS" /SECDrawAs="Region" /SECStyleId=1 misc_feature 8578..8831 /gene="NOS-T" /product="Nos-Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="NOS-T" /SECDescr="Nos-Terminator" misc_feature 8841..9293 /gene="U6Os'" /product="U6Os C4" /SECDrawAs="Region" /SECStyleId=1 CDS 9294..9322 /gene="insert target RNA" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 CDS 9323..9352 /product="hairpin" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 9323..9405 /product="gRNA scaffold" /SECDrawAs="Region" /SECStyleId=1 misc_feature 9491..9514 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 10049..10746 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 11034..13821 /gene="'pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 14005..15015 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg 1741 ggccccgagc gcaggcccgc cgacatccgc cgtgccaccg aggccgacat gccagccgtc 1801 tgcaccatcg tgaaccacta catcgagacc tccacggtca acttccgcac agagccgcag 1861 gagcctcagg agtggaccga cgacctcgtg aggctgcgcg agcgctaccc ctggctcgtc 1921 gctgaggtgg atggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggtaagtttc 1981 tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat ataatatttc 2041 aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct gtagtttata 2101 agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt tgatgtgcag 2161 gcacgcaacg cctacgactg gacggcggag agcaccgtct acgtgtcccc acgccaccag 2221 cgcaccggcc tgggctccac gctctacacc cacctgctca agagcctgga ggcccagggc 2281 ttcaagtccg tggtcgctgt gatcggcctc cccaacgacc cgagcgtccg tatgcacgag 2341 gccctcggct acgcgccccg cgggatgctg agggccgcgg gcttcaagca cggcaactgg 2401 cacgacgtgg gcttctggca gctcgacttc tccctgcctg tcccaccccg cccggtgctc 2461 cccgtcaccg aaatctgaac tccggaatcg cgttcgagta ttatggcatt gggaaaactg 2521 tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg gttttcgcta 2581 tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt ccttttgttc 2641 attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa tttgaaatta 2701 taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga 2761 ccgaagttaa tatgaggagt aaaacactga agcctgcagg catgcaagct gatccactag 2821 aggccatggc ggccgcacta gtctgcagtg cagcgtgacc cggtcgtgcc cctctctaga 2881 gataatgagc attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact 2941 tgtttgaagt gcagtttatc tatctttata catatattta aactttactc tacgaataat 3001 ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag 3061 acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta 3121 gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat 3181 tttattagta catccattta gggtttaggg ttaatggttt ttatagacta atttttttag 3241 tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt 3301 tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa 3361 atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg 3421 ccagcctgtt aaacgccgtc gatcgacgag tctaacggac accaaccagc gaaccagcag 3481 cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc 3541 tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg 3601 gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg 3661 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat 3721 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac 3781 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc 3841 gtcctccccc cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg 3901 cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc 3961 tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc 4021 cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatc 4081 taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca 4141 gcatctattc atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt 4201 ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt 4261 tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct 4321 caccctgttg tttggtgtta cttctgcagg tactagatcc tccaccgaaa tggcctccgg 4381 acccaagaag aagcgcaaag tgggaatcca cggagctcca gacaagaagt acagcattgg 4441 tctggacatc gggaccaact ccgtcggctg ggccgttatc accgatgagt acaaagtgcc 4501 ttctaagaag ttcaaagtcc tcggaaacac tgacaggcac agcatcaaga agaacttgat 4561 tggcgccctt ctcttcgact ccggcgaaac cgctgaggcc acccgtctga agcgcaccgc 4621 cagaaggcgc tacacccgtc gcaagaatag gatctgctac ctccaggaga tcttcagcaa 4681 cgagatggcc aaagtggacg attccttctt tcaccgtttg gaggagagct tccttgtcga 4741 ggaggacaag aagcacgagc gccatcccat cttcggaaac attgttgacg aggtggctta 4801 ccacgagaag tatccaacta tctaccacct cagaaagaag ctggtggact ccaccgacaa 4861 ggccgatttg aggctcatct accttgccct cgctcacatg atcaagttcc gcggtcactt 4921 cctgattgag ggcgacctca accccgacaa ctctgacgtc gataagttgt tcatccaact 4981 ggtccagacc tacaaccagc ttttcgagga gaatcctatc aacgcctccg gggttgacgc 5041 caaggctatt ctcagcgccc gtctgtccaa gagccgcagg cttgagaact tgatcgccca 5101 gctcccagga gagaagaaga acggcctctt cggaaacctg atcgctctct ccttgggcct 5161 tacccctaac ttcaagtcta attttgacct cgccgaggac gccaagctgc aactcagcaa 5221 ggacacctac gatgacgact tggacaacct gctcgcccag atcggcgatc agtacgctga 5281 ccttttcttg gccgccaaga acctctccga cgctattctg ctctccgaca tccttcgcgt 5341 gaacactgag atcaccaagg ccccactgag cgcctccatg atcaagcgtt acgacgaaca 5401 ccaccaagat ttgaccctcc tcaaggctct tgtcagacag cagctgcccg agaagtacaa 5461 ggagattttc ttcgaccaga gcaagaacgg atacgccggt tacatcgacg gcggggcctc 5521 ccaagaggag ttctacaagt tcatcaagcc catcttggag aagatggacg gcaccgaaga 5581 gctgctcgtg aagctcaacc gcgaggatct tttgaggaag cagcgtacct tcgacaacgg 5641 atctattccc caccagatcc atctcggcga gctgcacgct atcctccgca ggcaagagga 5701 cttctatcca ttccttaagg acaatcgcga gaagattgaa aagatcctca cttttagaat 5761 cccttactac gtcggaccct tggcccgtgg caacagccgc ttcgcctgga tgaccaggaa 5821 gtccgaggaa accatcaccc catggaactt cgaggaggtg gttgacaagg gggccagcgc 5881 tcagtccttc attgagcgca tgaccaactt cgataagaac ctgcccaacg agaaagtcct 5941 cccaaagcac tcccttctgt acgagtactt cactgtctac aatgagttga ccaaagtgaa 6001 gtacgtgacc gagggtatgc gtaagcctgc cttcctcagc ggcgagcaga agaaggccat 6061 cgttgacctc ctgttcaaga ccaacaggaa agtcaccgtg aagcagctta aggaggacta 6121 cttcaagaag atcgagtgct ttgactctgt cgagatcagc ggagtggagg atcgcttcaa 6181 cgcttccttg ggcacttacc acgacctcct gaagattatc aaggacaagg acttcctcga 6241 caacgaggag aacgaagata tcttggagga catcgttctt accctcaccc tgttcgagga 6301 cagagagatg attgaggaac gcctcaagac ctacgcccac ttgttcgacg ataaagtcat 6361 gaagcaactt aagcgtaggc gctacaccgg atggggcagg ctgtcccgta agctcatcaa 6421 cggtatccgc gacaagcaga gcggcaagac tattctcgac ttcttgaagt ccgacggatt 6481 cgccaatcgc aacttcatgc agctgatcca cgacgattct cttaccttca aggaggacat 6541 ccaaaaggct caagtgtccg gccagggcga cagcctccac gagcacatcg ccaacctggc 6601 cgggagcccc gctattaaga agggaatcct ccagaccgtc aaggtggttg acgagttggt 6661 caaggtgatg ggcagacata agccagagaa catcgtcatc gagatggcca gggaaaacca 6721 gaccacccag aagggacaga agaactcccg tgagcgcatg aagaggattg aggagggcat 6781 caaggagctt ggttcccaga tcctcaagga gcaccccgtg gaaaacaccc aactccagaa 6841 tgagaagctg tatttgtact accttcagaa cgggcgcgat atgtacgttg accaagagct 6901 ggacatcaac cgcctcagcg actacgacgt ggatcacatt gtccctcagt cctttctcaa 6961 ggacgactct atcgacaaca aggtgttgac tagaagcgat aagaaccgcg gcaagtccga 7021 caacgtccca tccgaggagg tggtcaagaa gatgaagaat tactggaggc agctgcttaa 7081 cgccaagctc atcacccagc gcaagttcga caacttgacc aaggccgagc gtggaggcct 7141 cagcgaactg gacaaggctg gattcatcaa gaggcaactt gttgaaaccc gccagattac 7201 caagcacgtg gcccagatcc tcgactcccg tatgaacact aagtacgatg agaacgacaa 7261 gctgatccgc gaggtcaaag tgattaccct caagagcaag ctcgtgtctg acttcagaaa 7321 ggacttccaa ttctacaagg ttagggagat caacaactac caccacgccc acgatgctta 7381 ccttaatgcc gtggtcggca ccgccttgat caagaagtac cccaagctgg agtccgagtt 7441 cgtgtatggt gactacaagg tctacgacgt tcgcaagatg atcgctaaga gcgagcagga 7501 gattggcaag gccaccgcca agtacttctt ctactccaac atcatgaact tctttaagac 7561 cgagatcact ttggctaacg gggagatccg taagcgccct ctcattgaaa ccaacggaga 7621 aaccggcgag atcgtgtggg acaagggcag ggacttcgcc accgtcagaa aagtgctgag 7681 catgccccaa gtcaacatcg tgaagaaaac cgaggttcag actggaggct tctccaagga 7741 gtctatcctc ccaaagcgca attccgataa gttgattgcc cgtaagaagg actgggaccc 7801 caagaagtac ggtggattcg acagcccaac cgtcgcctac tccgtgcttg tcgtggctaa 7861 agttgagaag ggcaagagca agaagctcaa gtccgtcaag gagctgctcg ggatcaccat 7921 catggagcgc agctccttcg agaagaaccc tattgatttc cttgaggcca agggctacaa 7981 ggaagtgaag aaggacttga tcatcaagct ccccaagtac tctctgttcg agcttgagaa 8041 cggaaggaag cgtatgctcg cctccgctgg cgagctgcaa aagggaaacg agttggccct 8101 cccaagcaag tacgtcaact tcctgtacct cgcctcccac tatgagaagc tcaagggcag 8161 ccccgaggac aacgaacaga agcagttgtt tgtggagcag cataagcact accttgacga 8221 gatcattgag cagatcagcg agttctccaa gcgcgttatc ctggctgacg ccaatctcga 8281 taaagtcctt tctgcctaca acaagcacag ggacaagcct atcagagagc aggctgaaaa 8341 cattatccac ttgttcaccc tcaccaacct gggtgcccca gccgccttca agtacttcga 8401 cactaccatc gaccgcaagc gttacacctc caccaaggaa gtgctcgatg ctacccttat 8461 ccaccagagc attactgggt tgtacgaaac caggatcgac ctgtcccaac tcggcggaga 8521 caagcgcccc gccgccacca agaaggctgg ccaggccaag aagaagaagt aacccgggat 8581 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg 8641 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg 8701 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg 8761 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg 8821 ttactagatc gctcgaattc gtacagcatt acgtaggtac gttttctttt tcttcccgga 8881 gagatgatac gataatcatg taaacccaga atttaaaaaa tattctttac tataaaaatt 8941 ttaattaggg aacgtattat tttttacatg acaccttttg agaaagaggg acttgtaata 9001 tgggacaaat gaacaatttc taagaaatgg gcatatgact ctcagtacaa tggaccaaat 9061 tccctccagt cggcccagca atacaaaggg aaagaaatga gggggcccac aggccacggc 9121 ccacttttct ccgtggtggg gagatccagc tagaggtccg gcccacaagt ggcccttgcc 9181 ccgtgggacg gtgggattgc agagcgcgtg ggcggaaaca acagtttagt accacctcgc 9241 tcacgcaacg acgcgaccac ttgcttataa gctgctgcgc tgaggctcag cttggtcttc 9301 ttcgaagctt ctgtgaagac cagttttaga gctagaaata gcaagttaaa ataaggctag 9361 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttgtcga caggccttaa 9421 gggccagatc ttgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact 9481 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa 9541 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg 9601 ccaaccacag ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca 9661 accacccaaa cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc 9721 gtctgggtcg gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg 9781 gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat 9841 ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac 9901 aggatgatct ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc 9961 cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc 10021 gccgccgacg ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa 10081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 10141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 10201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 10261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 10321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 10381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 10441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 10501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 10561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 10621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 10681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 10741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 10801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag 10861 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 10921 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 10981 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtaaccgtgc 11041 ggctgcatga aatcctggcc ggtttgtctg atgccaagct cgcggcctgg ccggcgagct 11101 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca 11161 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg 11221 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat 11281 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc 11341 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt 11401 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt 11461 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc 11521 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct 11581 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt 11641 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg 11701 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc 11761 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca 11821 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg 11881 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca 11941 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg 12001 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg 12061 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg 12121 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt 12181 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg 12241 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa 12301 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag 12361 gcggcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg 12421 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg 12481 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat 12541 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag 12601 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt 12661 tccgcagggc cggccggcat ggcgagtgtg tgggattacg acctggtact gatggcggtt 12721 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc 12781 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag 12841 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag 12901 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt 12961 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta 13021 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac 13081 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc 13141 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc 13201 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg 13261 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac 13321 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg 13381 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac 13441 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg 13501 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt 13561 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa 13621 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc 13681 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct 13741 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc 13801 cggcgcccac atcaaggcac cggtgggtat gcctgacgat gcgtggagac cgaaaccttg 13861 cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg 13921 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt 13981 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa 14041 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg 14101 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg 14161 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac 14221 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc 14281 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat 14341 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat 14401 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag 14461 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg 14521 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca 14581 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga 14641 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa 14701 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg 14761 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc 14821 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag 14881 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg 14941 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag 15001 gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt 15061 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt 15121 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat 15181 gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc 15241 ttggtcgttc cgcgtgaacg tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt 15301 gttgcgcgcc tgcccggtgc gtcggctgat ctcacggatc gactgcttct ctcgcaacgc 15361 catccgacgg atgatgttta aaagtcccat gtggatcact ccgttgcccc gtcgctcacc 15421 gtgttggggg gaaggtgcac atggctcagt tctcaatgga aattatctgc ctaaccggct 15481 cagttctgcg tagaaaccaa catgcaagct ccaccgggtg caaagcggca gcggc //