LOCUS       pLM                     1975 bp    DNA     circular SYN 25-JUN-2010
DEFINITION  Cloning of MCS into DCS5
ACCESSION   pLM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Amplification of MCS with primers M2AF x M2AR and M2BF x 
            M2BR and cloning of the MCS with the XbaI sites  into the SpeI site 
            of DCS5.
            Sequencing with primers:
            SF: Lox1-SF
FEATURES             Location/Qualifiers
     misc_feature    46..79
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Region"
     misc_feature    160..193
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Region"
     misc_feature    complement (243..909)
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
     CDS             complement (959..1819)
                     /gene="Amp"
                     /product="Amp"
                     /SECDrawAs="Gene"
ORIGIN      
        1 catttccccg aaaagtgcca ctagaggccc ttagggcctt aattaataac ttcgtataat
       61 gtatactata cgaagttatt gcggccgcac tagttggatc ctggccggcc ctgcagggaa
      121 ttccaagctt ggcgcgccga gctcagtcga ctggtaccta taacttcgta taatgtatac
      181 tatacgaagt tatttaaatt ggcccccaag gcctctagta aaaaggccgc gttgctggcg
      241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      901 gccatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gaccatgatt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctgctggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcatg gctgtcagac caagtttact catatatact ttagattgat
     1861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1921 ggcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgca
//