LOCUS p35S-Gusi-NosK 5828 bp DNA circular SYN 20-JUN-2013 DEFINITION 577p35S-Gusi-NosK rotated to position 5751 ACCESSION p35S-Gusi-NosK KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5828) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pGusi-BM* Fragment ends: HindIII and NotI Fragment size: 5263 Insert molecule: 35SNot-Hind* Fragment ends: NotI and HindIII Fragment size: 565 Primer for gene synthesis M13RY CACACAGGAAACAGCTATGAC p35SHind CCCTCTaagcttgagccatAATTCCTGCAGCCC Sequencing primers M13RY CACACAGGAAACAGCTATGAC FEATURES Location/Qualifiers misc_feature 105..617 /gene="35S'" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 651..1071 /gene="GUS" /product="GUS" /SECDrawAs="Gene" /SECStyleId=1 /SECName="GUS" /SECDescr="GUS" misc_feature 1072..1260 /gene="Intron" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Intron" /SECDescr="Intron" CDS 1261..2687 /gene="GUS" /product="GUS" /SECDrawAs="Gene" /SECStyleId=1 /SECName="GUS" /SECDescr="GUS" misc_feature 2748..3011 /gene="NOS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="NOS" /SECDescr="Terminator" misc_feature 3828..4688 /gene="Amp" /product="Ampecillin resistence" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="Ampecillin resistence" CDS 4731..5447 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagaataga ggacctaaca 121 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag 181 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa 241 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga 301 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 361 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 421 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 481 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 541 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 601 catttggaga gaacacgggg gactagtgga tcccccgggc tgcaggaatt atggctcaag 661 cttacgcgtg tcgacgtcag tcccttatgt tacgtcctgt agaaacccca acccgtgaaa 721 tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattggtc 781 agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta 841 acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg 901 aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca 961 ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata 1021 cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtaagtttc 1081 tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat ataatatttc 1141 aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct gtagtttata 1201 agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt tgatgtgcag 1261 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga 1321 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg 1381 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg 1441 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca 1501 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag 1561 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc 1621 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg 1681 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct 1741 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg 1801 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct 1861 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga 1921 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca 1981 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact 2041 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga 2101 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg 2161 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct 2221 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt 2281 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac 2341 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata 2401 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat 2461 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat 2521 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag 2581 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga 2641 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc aacaactctc 2701 ctggcgcacc atcgtcggct acagcctcgg gaattgctac cgagctcgaa tttccccgat 2761 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg 2821 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg 2881 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg 2941 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg 3001 ttactagatc gctcgacagg ccttaagggc cagatcttgg gcccggtacc caattcgccc 3061 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 3121 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 3181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 3241 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 3301 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 3361 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 3421 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 3481 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 3541 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 3601 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 3661 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg 3721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3841 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3901 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3961 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 4021 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 4081 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 4141 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 4201 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 4261 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 4321 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 4381 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4441 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4501 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4561 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4621 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4681 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4981 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 5041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 5101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 5161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 5221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 5281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 5341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 5401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5521 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 5641 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 5701 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 5761 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 5821 aacaattt //