LOCUS p35s-int-ocs 3834 bp DNA circular SYN 20-JUN-2013 DEFINITION Ligation of int-Xba-Bgl* into p35-OCS-BM* ACCESSION p35s-int-ocs KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3834) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: p35-OCS-BM* Fragment ends: BamHI and SpeI Fragment size: 3579 Insert molecule: int-Xba-Bgl* Fragment ends: XbaI and BglII Fragment size: 255 sequencing primers OR CATGCGATCATAGGCGTCTC FEATURES Location/Qualifiers misc_feature 106..527 /gene="35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="35S" /SECDescr="Promotor-Region" misc_feature 106..765 /gene="35s-int" /SECDrawAs="Region" /SECStyleId=1 misc_feature 577..765 /gene="int" /product="StLS1 intron to increase expression" /SECDrawAs="Region" /SECStyleId=1 misc_feature 823..1018 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 1834..2694 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 2737..3453 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc 121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gagttaatcc 541 atattggcag aacgaaaacg ctggttagca ccacaggtaa gtttctgctt ctacctttga 601 tatatatata ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa 661 aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta 721 atttataact tttctaatat atgaccaaaa tttgttgatg tgcaggtgac tagtgggatc 781 cagatccccc gggctgcagg aattcaagct tacgcgtgtc gaccctgctt taatgagata 841 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa 901 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat 961 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc 1021 gacaggcctt aagggccaga tcttgggccc ggtacccaat tcgccctata gtgagtcgta 1081 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 1141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 1201 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt 1261 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 1321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 1381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 1441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 1501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 1561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 1621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 1681 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 1801 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 1861 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 1921 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 1981 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 2041 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 2101 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 2161 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 2221 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 2281 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 2341 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 2401 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 2461 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 2521 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 2581 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 2641 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 2701 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 2761 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 2821 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 2881 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 2941 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3001 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 3061 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 3121 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 3181 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 3241 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3301 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 3361 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 3421 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 3481 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 3541 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 3601 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 3661 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 3721 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 3781 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca attt //