LOCUS pD35-ocs 3957 bp DNA circular SYN 20-JUN-2013 DEFINITION Ligation of D35-Eag-Bam* into pOCS-BM* ACCESSION pD35-ocs KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3957) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pOCS-BM* Fragment ends: BamHI and NotI Fragment size: 3145 Insert molecule: D35-Eag-Bam* Fragment ends: EagI and BamHI Fragment size: 812 Primer for gene synthesis D35Eag CCCTTcggccgCTAGATCGGGCCAA D35Bam CCCTggatccTactagtCGAGAGAGATAGATTTGTAGA Sequencing primers M13RY CACACAGGAAACAGCTATGAC OR CATGCGATCATAGGCGTCTC FEATURES Location/Qualifiers misc_feature 110..894 /gene="P2x35s'" /SECDrawAs="Region" /SECStyleId=1 misc_signal 822..828 /label=TATA /SECDrawAs="Label" misc_feature 946..1141 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 1957..2817 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 2860..3576 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgct agatcgggcc aacatggtgg 121 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg 181 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag 241 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc 301 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg 361 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc 421 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa 481 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga 541 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag 601 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc 661 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 721 gaccttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 781 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 841 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgact 901 agtaggatcc cccgggctgc aggaattcaa gcttacgcgt gtcgaccctg ctttaatgag 961 atatgcgaga cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa 1021 aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata 1081 tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt actgattgtc 1141 ctcgacaggc cttaagggcc agatcttggg cccggtaccc aattcgccct atagtgagtc 1201 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 1261 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 1321 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc 1381 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 1441 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 1501 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 1561 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 1621 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 1681 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 1741 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 1801 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 1861 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 1921 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 1981 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 2041 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 2101 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 2161 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 2221 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 2281 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 2341 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 2401 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 2461 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 2521 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 2581 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 2641 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 2701 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 2761 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 2821 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 2881 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 2941 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 3001 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 3061 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 3121 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 3181 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3241 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 3361 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3601 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3661 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 3721 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 3781 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 3841 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 3901 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaattt //