LOCUS       pENTA2                  2295 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  EntA2* Circularized
ACCESSION   pENTA2
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|EntA2 with EcoRI restricted and self ligated
            Primer for gene synthesis
            EMCSF	CCCgaattctaagcttgacgcgttagtcgaCCGACCCAGCTTTCT
            EMCSR	CCCgaattcactgcagacccgggtggatcctactagtCGGAGCCTGCTTTTTTGT
            Sequencing primers	
            M13FL	CGTTGTAAAACGACGGCCAGT
            M13R	CAGGAAACAGCTATGACC
FEATURES             Location/Qualifiers
     misc_feature    192..291
                     /gene="attL1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     352..354
                     /label=frame
                     /SECDrawAs="Label"
     misc_feature    353..452
                     /gene="attL2"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    553..1264
                     /gene="ColE1"
                     /product="Ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             complement(1312..2172)
                     /gene="amp"
                     /product="beta-lactamase, ampicillin resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggcca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
      121 cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat
      181 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
      241 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tccgactagt
      301 aggatccacc cgggtctgca gtgaattcta agcttgacgc gttagtcgac cgacccagct
      361 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
      421 gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagact ggccgtcgtt
      481 ttacaacgtc gtgactggga aaacatccat gctagcggcc aggaaccgta aaaaggccgc
      541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      961 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     1021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1621 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1921 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2281 atttccccga aaagt
//