LOCUS pUbi-BA 3522 bp DNA circular SYN 20-JUN-2013 DEFINITION Ligation of Ubi-Nos-BsiW-Sph* into 508pD2LC1* ACCESSION pUbi-BA KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3522) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: 508pD2LC1* Fragment ends: SphI and BsiWI Fragment size: 1684 Insert molecule: Ubi-Nos-BsiW-Sph* Fragment ends: BsiWI and SphI Fragment size: 1838 Primer for gene synthesis UbiBsiW CCCTcgtacgTGCAGTGCAGCGTGA NosSph CCCTTgcatGCGTCGAGCGATCTA Sequencing primers F CCTATGGAAAAACGCCAGC RN GGCTGACCTCCTATGCAGAT Sequencing primers UF1 CGAGTAGATAATGCCAGCC FEATURES Location/Qualifiers misc_feature 85..1592 /gene="'Ubi-int" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1071..1590 /gene="Intron" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1653..1906 /gene="NOS-T" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(1995..2659) /gene="ColE1''" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2437 /gene="LC" /product="Low copy origin" /SECDrawAs="Gene" /SECStyleId=1 /SECName="LC" /SECDescr="Low copy origin" CDS complement(2660..3522) /gene="Amp''" /product="Amp-DCS1" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggctgacctc ctatgcagat atcttatgag tgactagaat agctctcaag atcaggcctt 61 gggggccaat ttaaatggcg tacgtgcagt gcagcgtgac ccggtcgtgc ccctctctag 121 agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac 181 ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa 241 tataatctat agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta 301 gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt 361 agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca 421 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta 481 gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt 541 ttttttattt aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca 601 aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat 661 gccagcctgt taaacgccgt cgatcgacga gtctaacgga caccaaccag cgaaccagca 721 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc 781 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt 841 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg 901 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa 961 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca 1021 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct 1081 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg 1141 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg 1201 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg 1261 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat 1321 ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc 1381 agcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt 1441 tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat 1501 ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc 1561 tcaccctgtt gtttggtgtt acttctgcag gtactagtgg atcccccggg ctgcaggaat 1621 tcaagcttac gcgtgtcgac tcgaatttcc ccgatcgttc aaacatttgg caataaagtt 1681 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt 1741 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 1801 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 1861 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgctcg acgcatgctg 1921 gtacctgacg tctgcggccg cattaattaa ggccctaagg gcctgatctg tataaaggcc 1981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 2041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 2101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 2161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 2221 taggtcgttc gctccaagct gggctgtgag cacgaacccc ccgttcagcc cgaccgctgc 2281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 2341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 2401 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 2461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 2521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 2581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 2641 taagggattt tggccatgag atcaccaatg cttaatcagt gaggcaccta tctcagcgat 2701 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 2761 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 2821 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 2881 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 2941 gccagttaat agtttgcgca acgttgttgc cattgctgct ggcatcgtgg tgtcacgctc 3001 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 3061 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 3121 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 3181 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 3241 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 3301 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 3361 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgggcaccca actgatcttc 3421 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 3481 aaaaaaggga ataagggcga cacggaaatg ttgaatactc at //