LOCUS p75-GusRNAi 11808 bp DNA circular SYN 07-JAN-2002 DEFINITION Ligation of 35GusRNAi into pLH7500 ACCESSION p75-GusRNAi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11808) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Cloning of 35GusRNAi (SfiI) from pLN35-Gus-RNAi into the SFiI restriction sites of pLH7500 FEATURES Location/Qualifiers misc_feature 7..422 /gene="35S" /product="35S-Promotor" /SECDrawAs="Region" misc_feature 439..990 /gene="pat" /product="Basta-Resistenz" /SECDrawAs="Region" misc_feature 1013..1213 /gene="T35S" /product="Terminator" /SECDrawAs="Region" misc_feature 1264..1297 /gene="LoxP" /product="LoxP511" /SECDrawAs="Region" misc_feature 1313..1731 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" misc_feature 1739..2288 /gene="GUS-sense" /product="GUS-sense" /SECDrawAs="Region" misc_feature 2290..3291 /gene="Spacer-GUS" /product="Spacer Gus" /SECDrawAs="Region" misc_feature complement (3339..3890) /gene="GUS-AS" /product="GUS-Antisense" /SECDrawAs="Region" misc_feature 3904..4157 /gene="NOS-T" /product="NOS-T" /SECDrawAs="Region" misc_feature 4164..4197 /gene="LoxP" /product="LoxP511" /SECDrawAs="Region" misc_feature 4335 /gene="BR" /product="Border-right" /SECDrawAs="Marker" misc_feature 4840..5537 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" misc_feature 6086..9791 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" misc_feature 9975..10985 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Region" misc_feature 11520 /gene="BL" /product="Border-Left" /SECDrawAs="Marker" ORIGIN 1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct 61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg 121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct 181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca 241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa 301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac 361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga 421 ggacctcgag tggccaccat gggcccagaa cgacgcccgg ccgacatccg ccgtgccacc 481 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc 541 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg 601 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg 661 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtctcc 721 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg 781 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg 841 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag 901 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc 961 cgtccggtcc tgcccgtcac cgagatctga gatcacgcgt tctagtccgc aaaaatcacc 1021 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc 1081 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac 1141 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa 1201 ccaaaatcca gtgacctgca ggcatgcaag ctgatccact agaggccctt agggccttaa 1261 ttaataactt cgtataatgt atactatacg aagttattgc ggccgcacta gatcaacatg 1321 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccag 1381 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 1441 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc 1501 catcattgcg ataaaggaaa ggctatcgtt caagaatgcc tctaccgaca gtggtcccaa 1561 agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc 1621 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta 1681 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacactagt 1741 tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca 1801 gtctggatcg cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa 1861 gccgggcaat tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta 1921 attatgcggg caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc 1981 agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc 2041 aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg 2101 ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga 2161 ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc 2221 atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc acgccgaaca 2281 cctgggtgga tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg 2341 ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat 2401 aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt 2461 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg 2521 gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg 2581 ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg 2641 cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg 2701 tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca 2761 ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg 2821 ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt 2881 tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa 2941 gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg 3001 gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt 3061 gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag 3121 gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag 3181 aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc 3241 tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac 3301 tctcctggcg caccatcgtc ggctacagcc tcgggaattc cacccaggtg ttcggcgtgg 3361 tgtagagcat tacgctgcga tggattccgg catagttaaa gaaatcatgg aagtaagact 3421 gctttttctt gccgttttcg tcggtaatca ccattcccgg cgggatagtc tgccagttca 3481 gttcgttgtt cacacaaacg gtgatacgta cacttttccc ggcaataaca tacggcgtga 3541 catcggcttc aaatggcgta tagccgccct gatgctccat cacttcctga ttattgaccc 3601 acactttgcc gtaatgagtg accgcatcga aacgcagcac gatacgctgg cctgcccaac 3661 ctttcggtat aaagacttcg cgctgatacc agacgttgcc cgcataatta cgaatatctg 3721 catcggcgaa ctgatcgtta aaactgcctg gcacagcaat tgcccggctt tcttgtaacg 3781 cgctttccca ccaacgctga tcaattccac agttttcgcg atccagactg aatgcccaca 3841 ggccgtcgag ttttttgatt tcacgggttg gggtttctac aggacgtaac gagctcagtc 3901 gacgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 3961 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 4021 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa 4081 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 4141 tctatgttac tagatcggta cctataactt cgtataatgt atactatacg aagttattta 4201 aattggcccc catggccaga tctgggcccg gtacccgatc agattgtcgt ttcccgcctt 4261 cggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc 4321 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt 4381 gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg tgcgataatg 4441 acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga 4501 tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct tgatccctca 4561 acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg 4621 aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa 4681 agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat ccgggtctgt 4741 ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg 4801 cctgggcagt cgccgccgac gccgttgatg tggacgccga aaaggatcta ggtgaagatc 4861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 4921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 4981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 5041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 5101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 5281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 5581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgatta 5641 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 5701 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 5761 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 5821 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac 5881 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 5941 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 6001 gcaggggtac gtcgaggtcg atccaacccc tccgctgcta tagtgcagtc ggcttctgac 6061 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg 6121 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga 6181 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc 6241 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc 6301 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc 6361 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc 6421 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg 6481 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc 6541 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc 6601 gcacccggag cgggcgcgag gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta 6661 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg 6721 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc 6781 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat 6841 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa 6901 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat 6961 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga 7021 aatcctggcc ggtttgtctg atgccaagct cgcggcctgg ccggcgagct tggccgctga 7081 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca 7141 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg 7201 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat 7261 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag 7321 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc 7381 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc 7441 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg 7501 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg 7561 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg 7621 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg 7681 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc 7741 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc 7801 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca 7861 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca 7921 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca 7981 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat 8041 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg 8101 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc 8161 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc 8221 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc 8281 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg 8341 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg 8401 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga 8461 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca 8521 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac 8581 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc 8641 cggccggcat ggcgagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa 8701 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc 8761 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg 8821 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga 8881 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca 8941 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga 9001 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact 9061 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca 9121 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt 9181 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg 9241 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga 9301 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc 9361 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc 9421 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga 9481 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa 9541 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc 9601 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc 9661 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc 9721 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac 9781 atcaaggcac cggtgggtat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg 9841 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac 9901 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt 9961 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg 10021 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta 10081 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 10141 gcagcaacga tgttacgcag cagggcagtc gccctaaanc aaagttaaac atcatgaggg 10201 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 10261 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 10321 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 10381 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 10441 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 10501 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 10561 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 10621 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 10681 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 10741 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 10801 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 10861 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 10921 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 10981 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg 11041 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat 11101 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg 11161 ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc 11221 cgcgtgaacg tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc 11281 tgcccggtgc gtcggctgat ctcacggatc gactgcttct cttcgcaacg ccatccgacg 11341 gatgatgttt aaaagtccca tgtggatcac tccgttgccc cgtcgctcac cgtgttgggg 11401 ggaaggtgca catggctcag ttctcaatgg aaattatctg cctaaccggc tcagttctgc 11461 gtagaaacca acatgcaagc tccaccgggt gcaaagcggc agcggcggca ggatatattc 11521 aattgtaaat ggcttcatgt ccgggaaatc tacatggatc agcaatgagt atgatggtca 11581 atatggagaa aaagaaagag taattaccaa ttttttttca attcaaaaat gtagatgtcc 11641 gcagcgttat tataaaatga aagtacattt tgataaaacg acaaattacg atccgtcgta 11701 tttataggcg aaagcaataa acaaattatt ctaattcgga aatctttatt tcgacgtgtc 11761 tacattcacg tccaaatggg ggcttagatg agaaacttca cgatcggc //