LOCUS p9N-35S-Ubi10-RN 11717 bp DNA circular SYN 20-JUN-2013 DEFINITION Ligation of inverted 670pD1-35s-Ubi10-RNAi* into p9N* ACCESSION p9N-35S-Ubi10-RN KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11717) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: p9N* Fragment ends: SfiI Fragment size: 8970 Insert molecule: 670pD1-35s-Ubi10-RNAi* Fragment ends: SfiI Fragment size: 2747 FEATURES Location/Qualifiers misc_feature 7..304 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="Pnos" /SECDescr="Promotor" CDS 319..1122 /gene="NptII" /product="Kanamycin-Resistenz" /SECDrawAs="Gene" /SECStyleId=1 /SECName="NptII" /SECDescr="Kanamycin-Resistenz" misc_feature 1135..1336 /gene="T35S" /product="Terminator 35S" /SECDrawAs="Region" /SECStyleId=1 /SECName="T35S" /SECDescr="Terminator 35S" misc_feature 1395..1907 /gene="P35S" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(1921..1969) /gene="T35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1989..2538 /SECDrawAs="Gene" /SECStyleId=1 CDS complement(1989..2538) /gene="Gus" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2559..2607 /gene="T35S" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2621..4096) /gene="Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(2623..2926) /gene="int" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4192..4215 /gene="BR" /product="Border-right" /SECDrawAs="Region" /SECStyleId=1 /SECName="BR" /SECDescr="Border-right" misc_feature 4750..5447 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1 ORI" misc_feature 5996..9701 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="pVS1 ORI" /SECDescr="pVS1 ORI" misc_feature 9885..10895 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Sm/Sp" /SECDescr="Strep/Spec resistence" misc_feature 11416..11439 /gene="BL" /product="Border-Left" /SECDrawAs="Region" /SECStyleId=1 /SECName="BL" /SECDescr="Border-Left" ORIGIN 1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga 61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg 121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa 181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt 241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct 301 gcacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct 361 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 421 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 481 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 541 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 601 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 661 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 721 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 781 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 841 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 901 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 961 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 1021 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 1081 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc 1141 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag 1201 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga 1261 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta 1321 aaaccaaaat ccagtgacct gcaggcatgc aagctgatcc actagaggcc atgggggcca 1381 atttaaatgg taccaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc 1441 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac 1501 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt 1561 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc 1621 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc 1681 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc 1741 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg 1801 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa 1861 gacccttcct ctatataagg aagttcattt catttggaga gaacacggtc gactgagctc 1921 gttttaggaa ttagagattt tattgagagc agtattttac aaatacaaac gcgtctgtac 1981 ataagcttca cccaggtgtt cggcgtggtg tagagcatta cgctgcgatg gattccggca 2041 tagttaaaga aatcatggaa gtaagactgc tttttcttgc cgttttcgtc ggtaatcacc 2101 attcccggcg ggatagtctg ccagttcagt tcgttgttca cacaaacggt gatacgtaca 2161 cttttcccgg caataacata cggcgtgaca tcggcttcaa atggcgtata gccgccctga 2221 tgctccatca cttcctgatt attgacccac actttgccgt aatgagtgac cgcatcgaaa 2281 cgcagcacga tacgctggcc tgcccaacct ttcggtataa agacttcgcg ctgataccag 2341 acgttgcccg cataattacg aatatctgca tcggcgaact gatcgttaaa actgcctggc 2401 acagcaattg cccggctttc ttgtaacgcg ctttcccacc aacgctgatc aattccacag 2461 ttttcgcgat ccagactgaa tgcccacagg ccgtcgagtt ttttgatttc acgggttggg 2521 gtttctacag gacgtaacct taagacccgg gaggatcctt tgtatttgta tttatcagca 2581 cacaataaat acacattttc ctaaaacggc gccaactagt acctgttaat cagaaaaact 2641 cagattaatc gacaaattcg atcgcacaaa ctagaaacta acaccagatc tagatagaaa 2701 tcacaaatcg aagagtaatt attcgacaaa actcaaatta tttgaacaaa tcggatgata 2761 tctatgaaac cctaatcgag aattaagatg atatctaacg atcaaaccca gaaaatcgtc 2821 ttcgatctaa gattaacaga atctaaacca aagaacatat acgaaattgg gatcgaacga 2881 aaacaaaatc gaagattttg agagaataag gaacacagaa atttaccttg atcacggtag 2941 agagaattga gagaaagttt ttaagatttt gagaaattga aatctgaatt gtgaagaaga 3001 agagctcttt gggtattgtt ttatagaaga agaagaagaa aagacgagga cgactaggtc 3061 acgagaaagc taaggcggtg aagcaatagc taataataaa atgacacgtg tattgagcgt 3121 tgtttacacg caaagttgtt tttggctaat tgccttattt ttaggttgag gaaaagtatt 3181 tgtgctttga gttgataaac acgactcgtg tgtgccggct gcaaccactt tgacgccgtt 3241 tattactgac tcgtcgacaa ccacaatttc taacggtcgt cataagatcc agccgttgag 3301 atttaacgat cgttacgatt tatatttttt tagcattatc gttttatttt ttaaatatac 3361 ggtggagctg aaaattggca ataattgaac cgtgggtccc actgcattga agcgtatttc 3421 gtattttcta gaattcttcg tgctttattt cttttccttt ttgttttttt ttgccattta 3481 tctaatgcaa gtgggcttat aaaatcagtg aatttcttgg aaaagtaact tctttatcgt 3541 ataacatatt gtgaaattat ccatttcttt taatttttta gtgttattgg atatttttgt 3601 atgattattg atttgcatag gataatgact tttgtatcaa gttggtgaac aagtctcgtt 3661 aaaaaaggca agtggtttgg tgactcgatt tattcttgtt atttaattca tatatcaatg 3721 gatcttattt ggggcctggt ccatatttaa cactcgtgtt cagtccaatg accaataata 3781 ttttttcatt aataacaatg taacaagaat gatacacaaa acattctttg aataagttcg 3841 ctatgaagaa gggaacttat ccggtcctag atcatcagtt catacaaacc tccatagagt 3901 tcaacatctt aaacaagaat atcctgatct gaagaatgtg gaggctttag tcccttggat 3961 acttgggagg ctgtggaaga acagaaacga gctggtgctt aaagggaggg aatttggaac 4021 caatgaggta ttagtaagga cacaagaaga tgcagatgag tggattagaa ggaaagaggc 4081 tcagaatgta aggaaagcgg ccgcattaat taaggcccta agggccagat cttgggcccg 4141 gtacccgatc agattgtcgt ttcccgcctt cggtttaaac tatcagtgtt tgacaggata 4201 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg 4261 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct 4321 cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa acgtcggaaa 4381 gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc ggctagaagg 4441 tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa 4501 aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga tctgcttgtc gctctttcgg 4561 ccgacgtcct ggccagtcat cacgcgccaa agttccgtca caggatgatc tggcgcgagt 4621 tgctggatct cgccttcaat ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc 4681 agcaagatat cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac gccgttgatg 4741 tggacgccga aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 4801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 4861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 4921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 4981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 5041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 5101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 5161 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 5221 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 5281 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 5341 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 5401 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 5461 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 5521 ttatcccctg attctgtgga taaccgatta ccgcctttga gtgagctgat accgctcgcc 5581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5641 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5701 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5761 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5821 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5881 gttttcaccg tcatcaccga aacgcgcgag gcaggggtac gtcgaggtcg atccaacccc 5941 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 6001 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 6061 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 6121 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 6181 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 6241 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 6301 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 6361 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 6421 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 6481 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 6541 cgcgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 6601 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 6661 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 6721 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 6781 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 6841 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 6901 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 6961 cgcggcctgg ccggcgagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 7021 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 7081 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 7141 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 7201 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 7261 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 7321 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 7381 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 7441 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 7501 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 7561 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 7621 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 7681 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 7741 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 7801 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 7861 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 7921 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 7981 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 8041 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 8101 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 8161 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg 8221 acctggtgga gaagttgaag gcggcgcagg ccgcccagcg gcaacgcatc gaggcagaag 8281 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac 8341 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt 8401 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg 8461 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc 8521 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggcgagtgtg tgggattacg 8581 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga 8641 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc 8701 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca 8761 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat 8821 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg 8881 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc 8941 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc 9001 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga 9061 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc 9121 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc 9181 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat 9241 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct 9301 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt 9361 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa 9421 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa 9481 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc 9541 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg 9601 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg 9661 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cggtgggtat gcctgacgat 9721 gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc 9781 tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt 9841 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact 9901 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt 9961 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt 10021 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc 10081 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac 10141 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt 10201 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta 10261 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa 10321 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc 10381 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc 10441 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta 10501 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac 10561 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat 10621 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca 10681 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa 10741 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg 10801 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg 10861 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac 10921 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca 10981 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca 11041 caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa 11101 gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg tcggctcgat tgtacctgcg 11161 ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc gtcggctgat ctcacggatc 11221 gactgcttct ctcgcaacgc catccgacgg atgatgttta aaagtcccat gtggatcact 11281 ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac atggctcagt tctcaatgga 11341 aattatctgc ctaaccggct cagttctgcg tagaaaccaa catgcaagct ccaccgggtg 11401 caaagcggca gcggcggcag gatatattca attgtaaatg gcttcatgtc cgggaaatct 11461 acatggatca gcaatgagta tgatggtcaa tatggagaaa aagaaagagt aattaccaat 11521 tttttttcaa ttcaaaaatg tagatgtccg cagcgttatt ataaaatgaa agtacatttt 11581 gataaaacga caaattacga tccgtcgtat ttataggcga aagcaataaa caaattattc 11641 taattcggaa atctttattt cgacgtgtct acattcacgt ccaaatgggg gcttagatga 11701 gaaacttcac gatcggc //