LOCUS       pD1-35S-RNAi2           3321 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of Gus-Bsr-Xma* into pD1-35s-RNAi*
ACCESSION   pD1-35S-RNAi2
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pD1-35s-RNAi*
               Fragment ends: BsrGI and XmaI
               Fragment size: 2753
            Insert molecule:  Gus-Bsr-Xma*
               Fragment ends: XmaI and BsrGI
               Fragment size: 568
            Primer for gene synthesis
            Xma-XmaJ	CCTTcccgggTcctaGGTTACGTCCTGTAGAA
            Bsr-Eco	CCCTCtgtacaCgaattCACCCAGGTGTTC
            Sequencing primers	
            RNAi3R	GTTGGCGCCGTTTTAGGAAAAT
FEATURES             Location/Qualifiers
     misc_feature    77..498
                     /gene="Pro-35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(511..559)
                     /gene="T35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             579..1128
                     /gene="Gus"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(579..1128)
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1147..1195
                     /gene="T35s"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(1209..1721)
                     /gene="Pro-35S"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1804..2666
                     /gene="Amp"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    join(2667..3321,1..10)
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc actagatcaa catggtggag cacgacactc tcgtctactc caagaatatc
      121 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
      181 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
      241 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
      301 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
      361 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      421 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      481 tttcatttgg agaggacact agttggcgcc gttttaggaa aatgtgtatt tattgtgtgc
      541 tgataaatac aaatacaaag gatcctcccg ggtcctaggt tacgtcctgt agaaacccca
      601 acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt
      661 ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca
      721 ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg caacgtctgg
      781 tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc
      841 gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag
      901 ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta
      961 cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg
     1021 attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc
     1081 ggaatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgaa ttcgtgtaca
     1141 gacgcgtttg tatttgtaaa atactgctct caataaaatc tctaattcct aaaacgagct
     1201 cagtcgaccg tgttctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
     1261 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     1321 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     1381 catctttggg accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat
     1441 gatggcattt gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag
     1501 ctgggcaatg gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg
     1561 ccctttggtc ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct
     1621 ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taagagactc tgtatgaact
     1681 gttcgccagt ctttacggcg agttctgtta ggtcctctat tggtaccatt taaattggcc
     1741 cccaaggcct gatcttgaga gctattctag tcactcatat aatgtctgca taggaggtca
     1801 gccatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2041 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2341 atgccagcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2641 gcctcactga ttaagcattg gtgatctcat ggccaaaatc ccttaacgtg agttttcgtt
     2701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2881 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3301 atgctcgtca ggggggcgga g
//