LOCUS       pD1-35s-RNAi            3323 bp    DNA     circular SYN 09-OCT-2009
DEFINITION  Ligation of gus-Bam-Mlu* into 35S-RNAi-Bam-Mlu*
ACCESSION   pD1-35s-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  35S-RNAi-Bam-Mlu*
               Fragment ends: MluI and BamHI
               Fragment size: 2739
            Insert molecule:  gus-Bam-Mlu*
               Fragment ends: BamHI and MluI
               Fragment size: 584
FEATURES             Location/Qualifiers
     misc_feature    77..498
                     /gene="Pro-35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     misc_feature    complement (511..559)
                     /gene="T35S"
                     /SECDrawAs="Region"
     CDS             580..1129
                     /gene="Gus"
                     /SECDrawAs="Gene"
     CDS             complement (580..1129)
                     /gene="(null)"
                     /SECDrawAs="Gene"
     misc_feature    1149..1197
                     /gene="T35s"
                     /SECDrawAs="Region"
     misc_feature    complement (1211..1723)
                     /gene="Pro-35S"
                     /product="Promotor"
                     /SECDrawAs="Region"
     CDS             1806..2668
                     /gene="Amp"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
     misc_feature    2669..10
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc actagatcaa catggtggag cacgacactc tcgtctactc caagaatatc
      121 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
      181 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
      241 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
      301 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
      361 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      421 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      481 tttcatttgg agaggacact agttggcgcc gttttaggaa aatgtgtatt tattgtgtgc
      541 tgataaatac aaatacaaag gatcctcccg ggtcttaagg ttacgtcctg tagaaacccc
      601 aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg
      661 tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc
      721 aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg gcaacgtctg
      781 gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt
      841 cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca
      901 gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt
      961 acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt
     1021 gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc
     1081 cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtga agcttatgta
     1141 cagacgcgtt tgtatttgta aaatactgct ctcaataaaa tctctaattc ctaaaacgag
     1201 ctcagtcgac cgtgttctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     1261 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     1321 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     1381 tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc ctttatcgca
     1441 atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa agtgacagat
     1501 agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa aagtctcaat
     1561 tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg
     1621 ctccaccatg ttgacgaaga ttttcttctt gtcattgagt cgtaagagac tctgtatgaa
     1681 ctgttcgcca gtctttacgg cgagttctgt taggtcctct attggtacca tttaaattgg
     1741 cccccaaggc ctgatcttga gagctattct agtcactcat ataatgtctg cataggaggt
     1801 cagccatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2041 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2161 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2281 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2341 cgatgccagc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2641 gtgcctcact gattaagcat tggtgatctc atggccaaaa tcccttaacg tgagttttcg
     2701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2881 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3301 tgatgctcgt caggggggcg gag
//