LOCUS       pD1-Nap-RNAi            3123 bp    DNA     circular SYN 25-JUN-2010
DEFINITION  Ligation of inverted Nap-SalKpn* into 1.pD1-35s-Nap-RNAi*
ACCESSION   pD1-Nap-RNAi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    76..439
                     /gene="Nap"
                     /product="Promotor"
     misc_feature    complement (453..501)
                     /gene="T35S"
     CDS             522..1071
                     /gene="Gus"
     CDS             complement (522..1071)
                     /gene="(null)"
     misc_feature    1091..1139
                     /gene="T35s"
     misc_feature    complement (1158..1521)
                     /gene="Nap"
                     /product="Promotor"
     CDS             1606..2468
                     /gene="Amp"
                     /product="Amp-DCS1"
     misc_feature    2469..10
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc actagaattt atagactctc atcccctttt aaaccaactt agtaaacgtt
      121 ttttttttta attttatgaa gttaagtttt taccttgttt ttaaaaagaa tcgttcataa
      181 gatgccatgc cagaacatta gctacacgtt acacatagca tgcagccgcg gagaattgtt
      241 tttcttcgcc acttgtcact cccttcaaac acctaagagc ttctctctca cagcacacac
      301 atacaatcac atgcgtgcat gcattattac acgtgatcgc catgcaaatc tcctttatag
      361 cctataaatt aactcatccg cttcactctt tactcaaacc aaaactcatc aatacaaaca
      421 agattaaaaa catacacgaa ctagttggcg ccgttttagg aaaatgtgta tttattgtgt
      481 gctgataaat acaaatacaa aggatcctcc cgggtcttaa ggttacgtcc tgtagaaacc
      541 ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac
      601 tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc aattgctgtg
      661 ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc gggcaacgtc
      721 tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt
      781 ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt gatggagcat
      841 cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc cgggaaaagt
      901 gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc gccgggaatg
      961 gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat
     1021 gccggaatcc atcgcagcgt aatgctctac accacgccga acacctgggt gaagcttatg
     1081 tacagacgcg tttgtatttg taaaatactg ctctcaataa aatctctaat tcctaaaacg
     1141 agctcagtcg acctagttcg tgtatgtttt taatcttgtt tgtattgatg agttttggtt
     1201 tgagtaaaga gtgaagcgga tgagttaatt tataggctat aaaggagatt tgcatggcga
     1261 tcacgtgtaa taatgcatgc acgcatgtga ttgtatgtgt gtgctgtgag agagaagctc
     1321 ttaggtgttt gaagggagtg acaagtggcg aagaaaaaca attctccgcg gctgcatgct
     1381 atgtgtaacg tgtagctaat gttctggcat ggcatcttat gaacgattct ttttaaaaac
     1441 aaggtaaaaa cttaacttca taaaattaaa aaaaaaaacg tttactaagt tggtttaaaa
     1501 ggggatgaga gtctataaat tctggtacca tttaaattgg cccccaaggc ctgatcttga
     1561 gagctattct agtcactcat ataatgtctg cataggaggt cagccatgag tattcaacat
     1621 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1681 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1741 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1801 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     1861 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1921 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1981 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2041 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2101 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgccagc agcaatggca
     2161 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2221 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2281 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2341 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2401 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2461 tggtgatctc atggccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3121 gag
//