LOCUS       pD1-UbiH-2xStp-R        3087 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of inverted Kpn-UH-Mlu-BsrG* into A251pD1-UbiH-35s-RNAi*
ACCESSION   pD1-UbiH-2xStp-R
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  A251pD1-UbiH-35s-RNAi*
               Fragment ends: KpnI and BsrGI
               Fragment size: 2658
            Insert molecule:  Kpn-UH-Mlu-BsrG*
               Fragment ends: KpnI and BsrGI
               Fragment size: 419
            Sequencing Primer:
            GusF1         CAGGAAGTGATGGAGCATC
FEATURES             Location/Qualifiers
     misc_feature    77..420
                     /gene="UbiH"
                     /product="Promotor aus Sonnenblume"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(435..487)
                     /gene="T35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     453..462
                     /label=2xStp
                     /SECDrawAs="Label"
     CDS             508..1057
                     /gene="Gus"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(508..1057)
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1077..1129
                     /gene="T35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     1102..1111
                     /label=2xStp
                     /SECDrawAs="Label"
     misc_feature    complement(1144..1487)
                     /gene="UbiH"
                     /product="Promotor aus Sonnenblume"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1570..2432
                     /gene="Amp"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    join(2433..3087,1..10)
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc actagagtcg acaaaactta agaaagtcac caaaaagaaa gtcaaagacg
      121 gcaacggcaa cccagaagct acgttctctt tctagattct atgccctacc taccgcgtgg
      181 tgcacaacaa cttgaccccc atcattgact ctccaccgta cacgtgtcac gataccattt
      241 gttggtctcc taccacttac gtaacattaa tattccatcc gtgaccgcct cttcttcatc
      301 atcctataaa ttcaccttct tatctcttcc gctttcattc gttcatcatc atcacaatta
      361 acaacaatcc aaagcaatct tctctcatat tcttatctct tcctgtttcg cttcgtcttg
      421 gactagttgg cgccgtttta ggaaaatgtg tatttattta ttgtgtgctg ataaatacaa
      481 atacaaagga tcctcccggg tcttaaggtt acgtcctgta gaaaccccaa cccgtgaaat
      541 caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca
      601 gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa
      661 cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt atcagcgcga
      721 agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac
      781 tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac
      841 gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt
      901 ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga
      961 aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg
     1021 cagcgtaatg ctctacacca cgccgaacac ctgggtgaag cttatgtaca gacgcgtttg
     1081 tatttgtaaa atactgctct caataaataa aatctctaat tcctaaaacg agctcagtcg
     1141 acccaagacg aagcgaaaca ggaagagata agaatatgag agaagattgc tttggattgt
     1201 tgttaattgt gatgatgatg aacgaatgaa agcggaagag ataagaaggt gaatttatag
     1261 gatgatgaag aagaggcggt cacggatgga atattaatgt tacgtaagtg gtaggagacc
     1321 aacaaatggt atcgtgacac gtgtacggtg gagagtcaat gatgggggtc aagttgttgt
     1381 gcaccacgcg gtaggtaggg catagaatct agaaagagaa cgtagcttct gggttgccgt
     1441 tgccgtcttt gactttcttt ttggtgactt tcttaagttt tgtcgacggt accatttaaa
     1501 ttggccccca aggcctgatc ttgagagcta ttctagtcac tcatataatg tctgcatagg
     1561 aggtcagcca tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1621 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1681 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1741 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1801 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1861 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1921 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1981 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2041 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2101 accacgatgc cagcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2161 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2221 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2281 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2341 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2401 ataggtgcct cactgattaa gcattggtga tctcatggcc aaaatccctt aacgtgagtt
     2461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3061 tttgtgatgc tcgtcagggg ggcggag
//