LOCUS pLN35-Gus-RNAi 4761 bp DNA circular SYN 13-JUL-2010 DEFINITION Ligation of GusAS into pLN35-GusS-SG ACCESSION pLN35-Gus-RNAi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4761) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software FEATURES Location/Qualifiers misc_feature 46..79 /gene="loxP" /product="LoxP511" misc_feature 95..513 /gene="P35s" /product="Promotor-Region" CDS 521..1070 /gene="GUS-sense" /product="GUS-sense" misc_feature 1072..2073 /gene="Spacer-GUS" /product="Spacer Gus" CDS complement (2121..2672) /gene="GUS-AS" /product="GUS-Antisense" misc_feature 2686..2939 /gene="Tnos" /product="NOS-T" misc_feature 2946..2979 /gene="LoxP" /product="LoxP511" misc_feature complement (3029..3695) /gene="ColE1" /product="ColE1" CDS complement (3745..4605) /gene="Amp" /product="Amp" ORIGIN 1 catttccccg aaaagtgcca ctagaggccc ttagggcctt aattaataac ttcgtataat 61 gtatactata cgaagttatt gcggccgcac tagatcaaca tggtggagca cgacactctc 121 gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt 181 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc 241 atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga 301 aaggctatcg ttcaagaatg cctctaccga cagtggtccc aaagatggac ccccacccac 361 gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 421 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc 481 ctctatataa ggaagttcat ttcatttgga gaggacacta gttacgtcct gtagaaaccc 541 caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact 601 gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc 661 caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct 721 ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt 781 tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc 841 agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg 901 tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg 961 tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg 1021 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gatccggtca 1081 gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt tactggcttt 1141 ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg ataacgtgct gatggtgcac 1201 gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac 1261 gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct 1321 gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa 1381 ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa 1441 gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa 1501 ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt 1561 aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc 1621 gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc 1681 caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag 1741 gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg 1801 cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat 1861 caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat 1921 tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat cttcactcgc 1981 gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc 2041 ggtgaaaaac cgcagcaggg aggcaaacaa tgaatcaaca actctcctgg cgcaccatcg 2101 tcggctacag cctcgggaat tccacccagg tgttcggcgt ggtgtagagc attacgctgc 2161 gatggattcc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt 2221 cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa 2281 cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg 2341 tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag 2401 tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt ataaagactt 2461 cgcgctgata ccagacgttg cccgcataat tacgaatatc tgcatcggcg aactgatcgt 2521 taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct 2581 gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga 2641 tttcacgggt tggggtttct acaggacgta acgagctcag tcgacgatcg ttcaaacatt 2701 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 2761 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 2821 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 2881 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg 2941 tacctataac ttcgtataat gtatactata cgaagttatt taaattggcc cccaaggcct 3001 ctagtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 3661 acgaaaactc acgttaaggg attttggcca tgagacaata accctgataa atgcttcaat 3721 aatattgaaa aaggaagacc atgattacca atgcttaatc agtgaggcac ctatctcagc 3781 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 3841 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 3901 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 3961 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4021 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gctggcatcg tggtgtcacg 4081 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 4141 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 4201 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 4261 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 4321 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 4381 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 4441 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 4501 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 4561 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatggctg tcagaccaag 4621 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 4681 tgaagatcct ttttgataat ctcatgggcg gatacatatt tgaatgtatt tagaaaaata 4741 aacaaatagg ggttccgcgc a //