Price list – DNA Cloning Service

aVectors and Bacteria

1000
Vectors from the website
250 €
1001
Special vectors from the website
300 €
1002
Vectors adapted to customers
on request
1003
RNAi or recombinase vectors
250 €
1004
Agrobacterium strain GV3101-pMP90RK
200 €
1005
Cas9 vectors
400 €
1006
DCS blue supercompetent cells, Transformation rate 1x109/µg DNA 48X50 µl single shot
192 €

bCloning from plasmids

2010
Cloning of a DNA fragment into a vector of your choice. (sequencing not included)
250 €
2020
Cloning of a second DNA fragment into the same vector (sequencing not included)
200 €
2021
Cloning of a third DNA fragment into the same vector (sequencing not included)
175 €
2030
or cloning of the same DNA fragment into another vector (sequencing not included)
200 €
2040
or cloning of a DNA fragment into a predigested vector (sequencing not included)
200 €
2050
Cloning of a DNA fragment into a vector with the same selection marker (sequencing not included)
300 €
2051
Cloning of a second DNA fragment into a vector with the same selection marker (sequencing not included)
250 €
2052
Cloning of a third DNA fragment into a vector with the same selection marker (sequencing not included)
225 €
2060
Seamless cloning of a DNA fragment into a vector of your choice. (sequencing not included)
350 €

cCloning of amplified DNA fragments (from plasmids or genomic DNA) up to 5 kb

2110
Cloning of a amplified DNA fragment into a vector of your choice. (sequencing and primers not included)
350 €
2111
Cloning of a second amplified DNA fragment into the same vector (sequencing and primers not included)
300 €
2112
Cloning of three amplified DNA fragments into the same vector (sequencing and primers not included)
275 €
2120
or cloning of the same amplified DNA fragment into another vector (sequencing and primers not included)
300 €
2121
or cloning of the same amplified DNA fragment into two other vectors (sequencing and primers not included)
275 €
2140
Cloning of amplified DNA into a predigested vector (sequencing not included)
300 €
2160–2162
Cloning of amplified DNA into a TA cloning vector

1 sample
250 €
2-3 samples
200 €
4-9 samples
175 €
2170
PCR-Cloning of DNA fragments larger 3.500 bp (1,00 € per 100 bp) (sequencing and primers not included)
350 €
2180
Cloning of DNA into a pUC cloning vector (sequencing not included)
300 €

dSite directed mutagenesis and DNA assembling, Gene Synthesis

2200
Site directed mutagenesis from plasmids (1 point mutation) (sequencing and primers not included)
300 €
2205
Site directed mutagenesis from plasmids (1 complex mutation) (sequencing and primers not included)
350 €
2210
Site directed mutagenesis from plasmids ( a second clone) (sequencing and primers not included)
250 €
2220
Site directed mutagenesis from plasmids (2 point mutations) (sequencing and primers not included)
450 €
2230
Site directed mutagenesis from plasmids (3 point mutations) (sequencing and primers not included)
550 €
2240
Site directed mutagenesis from plasmids (4 point mutations) (sequencing and primers not included)
650 €
2300
Assembling of 2 DNA fragments and cloning into a vector of your choice (sequencing and primers not included)
460 €
2301
Assembling of 3 DNA fragments and cloning into a vector of your choice (sequencing and primers not included)
570 €
2302
Assembling of 4 DNA fragments and cloning into a vector of your choice (sequencing and primers not included)
680 €
2303
Assembling of 5 DNA fragments and cloning into a vector of your choice (sequencing and primers not included)
790 €
2310
Assembling of 2 DNA fragments (sequencing and primers not included)
110 €
2400
Cloning of a amplified DNA fragment into a specific amplified vector (sequencing and primers not included)
450 €
2500
Cloning of a DNA fragment into a vector with specific restriction enzymes
350 €
2600
Cloning of 1 target site into a Cas vector (sequencing and primers included)
350 €
2601
Cloning of a second target site into a Cas vector (sequencing and primers included)
300 €
2602
Cloning of a third target site into a Cas vector (sequencing and primers included)
275 €
2610
Cloning of 1 double target site into a Cas vector (sequencing and primers included)
400 €
2611
Cloning of 2 double target sites into a Cas vector (sequencing and primers included)
350 €
2612
Cloning of 4 double target sites into a Cas vector (sequencing and primers included)
325 €
2613
Cloning of 8 double target sites into a Cas vector (sequencing and primers included)
300 €
2700
Cloning of 1 fourfold target sites into a Cas vector (sequencing and primers included)
1.200 €
2800
Cloning of 1 sixfold target sites into a Cas vector (sequencing and primers included)
2.000 €
2810
Cloning of 1 eightfold target sites into a Cas vector (sequencing and primers included)
2.800 €
2900
Cloning of RVD into binary Talen construct
50 €

eAdditional costs for cloning: If neccessary

3050
Codon optimization for high expression
0,10 €
3050
Codon optimization for optimum expression
0,15 €
3060
Elongation of PCR product
50 €
3090
Selection of bacteria with uncommon selection marker hygromycine
50 €
3090
Cloning into E. coli strains other than XL1
50 €
3105
Cloning of plasmids with missing sequence
50 €
3110
Cloning into low-copy plasmids additionally
50 €
3125
Cloning of DNA fragments larger 2.5 kb
50 €
3126
Cloning of DNA fragments larger 5 kb
100 €
3127
Cloning of DNA fragments larger 7.5 kb
150 €
3128
Cloning of DNA fragments larger 10 kb
200 €
3130
Blunt-end cloning one site
50 €
3145
Cloning into a single restriction site without blue-white selection.
50 €
3150
Cloning of a DNA fragment smaller 100 bp
25 €
3160
Cloning into vectors larger 10 kb
25 €
3170
In frame cloning
50 €
3180
Blunting of a restriction site
50 €
3190
Restriction of DNA fragments with uncommon or expensive enzymes like: AgeI, AvrII, PciI, SphI, XmaI, ClaI- each site costs of the Enzymes
0 €
3195
Isolation of a second clone
25 €
3196
Correction of electronic sequences each corrected bp
5 €

Primer

3210
Primer for DNA amplification and sequencing per base
0,50 €
3220
HPLC-purified primer per base (recomended for primers >30 bp)
1 €
3230
Phosphorylated primer per base
1 €
3240
Primer design
12 €
3241
Primer design and synthesis for sequencing
20 €

fAdditional service

Cloning

4000
Preparation of plasmid DNA for cloning
50 €
4100–4110
Genesynthesis with primers per base and cloning into a basic pUC vector

for genes smaller 625 bp
250 €
for genes larger 625 bp
0,35 €
4150
cDNA synthesis from mRNA
500 €
4160
5´Race sequencing included
700 €
4170
3´Race sequencing included
500 €
4210
Amplification and cloning of unknown genes with degenerated primers
on request
4310–4311
Promotor isolation from cDNA-sequences -Isolation of genomic sequences with inverse PCR-

1 sample
1.500 €
2 samples
1.000 €
4410
Preparation of a cloning plan each cloning step
50 €
4510
Documentation
50 €
4511
Certification
100 €
4512
Certification gene synthesis with primers
200 €
4512
Preparation of electronic files due to changed sequences
100 €

Transformation/Amplification

4600–4602
Transformation of DNA into E. coli

1 sample
25 €
2-9 samples
20 €
>10 samples
15 €
4610–4611
Streaking bacteria on a agar plate for antibiotic selection

1-10 samples
10 €
>10 samples
5 €
4630–4631
Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli

1 sample
150 €
2 samples
100 €
4710–4711
Amplification of DNA with primers

1 sample
20 €
>10 samples
10 €
4720
High quality amplification from genomic DNA 1 sample
100 €

DNA/RNA isolation

4750
Isolation of genomic DNA from tissue 1 sample
400 €
4800
Isolation of DNA/RNA from cells-Mini 1 sample
100 €
4801
Isolation of DNA/RNA from cells-Maxi 1 sample
200 €
4802
Isolation of DNA/RNA from cells-Mini 100 samples
20 €
4803
Pesteling of probes 100 samples
15 €
4804
RNA extraction 100 samples
10 €
4805
Probe preparation for extraction 100 samples
5 €
4820–4821
Agarose gel purification

1 sample
50 €
>10 samples
20 €
4830
Spin column purification 1 sample
10 €
4840
Restriction digest 1 sample
20 €
4845
UV measurement at 260 nm and DNA adjustment 1 sample
10 €
4850
UV measurement at 260 nm 1 sample
5 €
4855
Adjustment of DNA to 5 ng/µl 1 sample
100 €
4870
Dilution of DNA to 1x106 copys each sample
100 €
4880
Filling in tubes each sample
20 €
4890
Storage of the DNA each sample
100 €
4860
Agarose gel analysis 1 sample
10 €
4880
Removal of genomic E. coli DNA from Maxiprep 1 sample
200 €
4900–4902
E. coli Miniprep

1 sample
20 €
2-10 samples
15 €
>10 samples
10 €
4905
Colony PCR >10 samples
5 €
4910
Restriction and religation for Maxiprep 1 sample
100 €
4920–4927
Isolation of Maxi-Prep DNA (up to 500 µg) high copy

1 sample
140 €
2 samples
130 €
3 samples
125 €
4 samples
120 €
6 samples
115 €
8 samples
110 €
12 samples
105 €
16 samples
100 €
4930–4931
Isolation of Mega-Prep DNA (up to 2.000 µg)

1 sample
400 €
2 samples
350 €
4940
Isolation of Giga-Prep DNA (up to 10.000 µg) 1 sample
1.500 €
4950
Isolation of 60mg DNA 1 sample
4.000 €
4960–4963
Isolation of Maxi-Prep DNA (up to 200 µg) low copy

1 sample
195 €
2 samples
180 €
4 samples
165 €
8 samples
150 €
4970–4972
Isolation of Maxi-Prep DNA (up to 500 µg) Animal component free

1 sample
250 €
2 sample
220 €
3 sample
200 €

gSequencing price per sample (average reading length: 20-1000bp/99%)

5105
Sequencing primer 1 primer
20 €
5106
Primer for Gene synthesis (30 bp) 1 primer
30 €
5110
Column DNA purification and sequencing 100 samples
6,50 €
5120–5130
Sequencing: normal

11-100 samples
9 €
101-1000 samples
8 €
5410
Sequencing and comparison with a known sequence or storage of the sequence each sample
20 €
5415
Comparison with a known sequence each sample
5 €
5421
Full documentation of the DNA in gene bank format
50 €
5422
Sequencing of unknown DNA and documentation 1000 bp
100 €
5430
Import of sequences from NCBI
10 €
5510
DNA isolation, amplification and sequencing of genes from genomic DNA (16S-RNA, rubisco etc.)
200 €

hSupercompetent cells from your E. coli strain

6110–6112
Preparation of supercompetent cells from your E. coli strain in 2 ml vials (Transformation rate in 2ml vials: 1x109)

128 vials
384 €
64 vials
192 €

iPlant transformation

7001
Transformation into Arabidopsis
500 €
7002
Transformation into Arabidopsis (T1 seeds tested)
750 €
7003
Transformation into Arabidopsis (T2 seeds)
1.250 €
8000
mRNA-synthesis with capping and Tailing from Plasmid, 50 µg
450 €
8001
mRNA-synthesis with capping and Tailing from Plasmid, 100 µg
1.000 €
8002
mRNA-synthesis with capping and Tailing from Plasmid, 150 µg
1.200 €
8003
mRNA-synthesis with capping and Tailing from Plasmid, 200 µg
1.400 €
8004
mRNA-synthesis with capping and Tailing from Plasmid, 300 µg
1.800 €
8005
mRNA-synthesis with capping and Tailing from Plasmid, 400 µg
2.200 €
8006
mRNA-synthesis with capping and Tailing from Plasmid, 600 µg
3.000 €

Setup costs

When amplification failed:
costs for primer
When cloning failed: Due to our experience 99% of our cloning experiments are succesful
50 % of cloning costs

miscellaneous

10001
Delivery on ice
30 €
10001
Delivery on dry ice
20 €
10002
Delivery with FedEx
50-100 €
10003
Delivery with Expeditors
150 €
10004
payment with cheque
10 €
10005
payment with PayPal in Euro
5%
10006
PayPal payment in other currencies
10%
10006
payment with wire transfer
free
10007
Export handling
50 €
10008
Special Invoice Handling
50 €
10009
Additional Cost estimation
50 €

Good to know

Prices are without 19% VAT
If you send us plasmid DNA for cloning, we prefer it purified by a midi/maxi prep in TE-buffer
We need a minimum of 2µg vector DNA at a minimum concentration of 200ng/µl
Other plasmids should be already transformed in E. coli on a agar plate.
As a host strain we prefer DH5-alpha, XL1-blue or JM109
Generel cloning conditions will be valid for restriction enzymes with an overhang of
at least 3 bases, DNA fragments up to 2.000 bp and high-copy plasmids.
We don´t take any guaranty for genomic sequences
DNA data should be readable by a computer