Protocols
The methods we use every day, written down the way we do them.
- E. coli TransformationFive-minute heat-shock transformation of chemically competent cells, and which antibiotics it works with.
- Agrobacterium StrainsThe strain we use, GV3101-pMP90RK, and a comparison table of common Agrobacterium strains, backgrounds and markers.
- Agrobacterium transformationDirect transformation of GV3101 with binary vectors: competent cells, freeze-thaw, selection.