DESCRIPTION
Specifications (Cat. # DFH100, DFH300)
- High Efficiency: up to 90% recovery from agarose gels, up to 95% recovery from PCR or other enzymatic reactions
- Only 20 minute gel extraction and 10 minute PCR cleanup
- Convenient: Gel/PCR binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
- Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
- Sample Size: up to 300 mg of agarose gel slice or up to 100 μl of PCR products
- Broad Fragment Size Range: 70 bp–20 kb
- Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup
- Elution Volume: 20-50 µl
- Storage: dry at room temperature (15-25°C)
Applications
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, TransfectionComponents
- Gel/PCR Buffer (premixed with a pH indicator)
- 3M Sodium Acetate (pH5.0)
- W1 Buffer
- Wash Bufffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- DFH Columns
- 2 ml Collection Tubes
QUALITY CONTROL
The quality of the GenepHlow™ Gel/PCR Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions analyzed by electrophoresis.GenepHlow™ Gel/PCR Kit Functional Test Data
Figure 1 .Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel/PCR Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel.M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng)
TestDNA Conc.260/280YieldRecovery
| 1 | 18.2 ng/µl | 1.84 | 910ng | 82% |
| 2 | 18.7 ng/µl | 1.82 | 935ng | 85% |
| 3 | 19.2 ng/µl | 1.82 | 960ng | 87.3% |
Figure 2 .PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel/PCR Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1073 ng)
TestDNA Conc.260/280YieldRecovery
| 1 | 19.7 ng/µl | 1.86 | 985ng | 91.8% |
| 2 | 20.3 ng/µl | 1.85 | 1015ng | 94.6% |
| 3 | 20.5 ng/µl | 1.86 | 1025ng | 95.5% |
Figure 3. A pH indicator is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.
| Specifications | GenepHlow™ Gel Extraction Kit | GenepHlow™ PCR Cleanup Kit | GenepHlow™ Gel/PCR Kit | GenepHlow™ DNA Cleanup Maxi Kit | Presto™ 96 Well Gel Extraction Kit | Small DNA Fragments Extraction Kit | Large DNA Fragments Extraction Kit |
|---|---|---|---|---|---|---|---|
| Cat. # | DFG100 DFG300 | DFC100 DFC300 | DFH100 DFH300 | DFM010 DFM025 | 96DFG04 96DFG10 | DF101 DF301 | DFL100 DFL300 |
| Format | Spin Column | Spin Column | Spin Column | Maxi Spin Column | 96 Well Plate | Spin Column | Silica Matrix |
| Binding Capacity | 10 µg | 10 µg | 10 µg | 200 µg | 10 µg | 10 µg | 5 µg |
| Sample | Agarose Gel Slice | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/Restriction Enzyme Digest Product | Agarose Gel Slice | Agarose Gel Slice/PCR Product | Agarose Gel Slice/PCR Product |
| Fragment Size | 70 bp-20 kb | 70 bp-20 kb | 70 bp-20 kb | 100 bp-10 kb | 70 bp-20 kb | 40-200 bp | 100 bp-50 kb |
| pH Indicator | Yes | Yes | Yes | Yes | N/A | N/A | Yes |
| Recovery | Up to 90% | Up to 95% | Up to 95% | Up to 80% | Up to 80% | Up to 95% | Up to 95% (<10 kb) Up to 80% (10-50 kb) |
| Elution Volume | 20-50 µl | 20-50 µl | 20-50 µl | 500-1000 µl | 60-80 µl | 20-50 µl | 10-20 µl |
| Operation Time | 20 minutes | 10 minutes | <20 minutes | <25 minutes | 45 minutes | <20 minutes | <20 minutes |