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Presto Mini Plasmid Kit

For the Free Kit please write us an E-Mail to info@dna-cloning.com DNA Extraction/Plasmid DNA Purification This miniprep Plasmid Kit was designed for plasmid DNA purification from 1-7 ml of cultured bacterial cells.

DESCRIPTION

Specifications (Cat. # PDH100, PDH300)

  • Purify plasmid DNA within 15 minutes!
  • Convenient: TrueBlue Lysis Buffer is an optional color indicator included with the kit to reduce common handling errors, while ensuring efficient cell lysis, neutralization and maximum plasmid product yield so you can feel confident with each and every plasmid prep.
  • Plasmid Size: 1-15 kb
  • High Yield: up to 50 µg of pure plasmid DNA
  • Sample Volume: 1-7 ml of cultured bacterial cells
  • Spin Columns: glass fiber membrane optimized for plasmid DNA purification
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)

Applications

Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

Components

  • PD1, PD2, PD3 Buffers
  • TrueBlue Lysis Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • RNase A
  • PDH Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ Mini Plasmid Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Presto™ Mini Plasmid Kit. Following the purification process, a yield of more than 30 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Presto™ Mini Plasmid Kit Functional Test Data

E. coli Strain Cell Culture Volume Yield (OD600 = 4.0)
1.5 ml 3 ml 5 ml 7 ml
DH5α 13-15 µg 27-29 µg 36-38 µg 40-42 µg
Top 10 10-12 µg 18-20 µg 25-27 µg 27-29 µg
BL21 (DE3) 7-9 µg 13-15 µg 17-19 µg 20-22 µg
Table 1. Plasmid DNA was extracted using the Presto™ Mini Plasmid Kit. 10 µl aliquots of a 100 µl eluate of purified super coiled plasmid DNA from 1.5, 3, 5 and 7 ml overnight E. coli DH5α, TOP10 and BL21(DE3) cultures, containing a 3 kb plasmid pBluescript (OD600 = 4 U/ml) were analyzed by spectrophotometer.
Figure 1. Plasmid DNA was extracted using the Presto™ Mini Plasmid Kit. 5 µl aliquots of a 100 µl eluate of purified super coiled plasmid DNA from 1.5, 3, 5 and 7 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript and pBR322 (OD600 = 4 U/ml) were used in EcoRI digestion and analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder pBluescript (1.=1.5 ml, 2=3 ml, 3=5 ml, 4=7 ml) pBR322 (5=1.5 ml, 6=3 ml, 7=5 ml, 8=7 ml)
Copy Number Cell Culture Volume Yield (OD600 = 4.0)
1.5 ml 3 ml 5 ml 7 ml
High-Copy (pBluescript) 13-15 µg 27-29 µg 36-38 µg 40-42 µg
Low-Copy (pBR322) 4-6 µg 8-10 µg 12-14 µg 18-20 µg

Presto™ Mini Plasmid Kit Sequencing Data

Figure 2 .Sequencing data of (pBluescript) plasmid DNA purified using the Presto™ Mini Plasmid Kit.

TrueBlue Lysis Buffer Optional Color Indicator

Cell Lysis
Insufficient Mixing
Correct Mixing
Neutralization
Insufficient Mixing
Correct Mixing
Figure 3 .TrueBlue Lysis Buffer is an optional color indicator included with this Plasmid Kit to eliminate common handling errors and ensure efficient cell lysis and neutralization each and every time.

Plasmid DNA Kit Specifications

Specifications Presto™ Endotoxin Free Mini Plasmid Kit Presto™ Mini Plasmid Kit High-Speed Plasmid Mini Kit Large Plasmid DNA Extraction Kit Presto™ 96 Well Plasmid Kit
Cat. # PEH100 PDH100 PDH300 PD100 PD300 PDL100 PDL300 96PDC04/10 96PDV04/10
Format Spin Column Spin Column Spin Column Spin Column 96 Well Plate
Binding Capacity 40 µg 50 µg 30 µg 30 µg 35 µg
Culture Input 1-5 ml 1-7 ml 1-5 ml 1-4 ml 1-5 ml
Plasmid Size 1-15 kb 1-15 kb 1-15 kb 10-50 kb 1-15 kb
Typical Yield up to 40 µg up to 50 µg 10-30 µg 10-25 µg 25-35 µg/well
Elution Volume 30-100 µl 30-100 µl 30-100 µl 50-100 µl 100 µl
Operation Time <60 minutes <15 minutes <15 minutes <15 minutes 25 minutes