DNA Cloning Service

Binaries

p4o2x35s-UbiZm-LH B512

Ligation of 594pUbi-BA* into B481p4o2x35s-LH*

24681012pVS1UbiZmahasSm/SpP2x35sColE1ahasintTE9TnosintRBLBB512p4o2x35s-Ubi12,856 bp
Promoter 1
2x35s
Gene 1
AHAS
Promoter 2
UbiZm
Gene 2
MCS
Features
monocot
Size
12,856 bp, circular
Record date
08-FEB-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P2x35s Promoter misc_feature 3141,098785 +
ahas Coding sequence CDS 1,1161,754639 +
int Site CDS 1,7551,943189 +
ahas Coding sequence CDS 1,9443,3171,374 +
TE9 Terminator misc_feature 3,3353,625291 +
UbiZm Promoter misc_feature 3,6895,1961,508 +
int Site misc_feature 4,6755,194520 +
Tnos Terminator misc_feature 5,2575,510254 +
RB T-DNA border misc_feature 5,6335,65624 +
ColE1 Origin / backbone misc_feature 6,1916,888698 +
pVS1 Origin / backbone misc_feature 7,43711,1423,706 +
Sm/Sp Selection marker CDS 11,32612,3361,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.