DNA Cloning Service

Binaries

p5o2x35s-UbiZm-LH B513

Ligation of 594pUbi-BA* into B482p5o2x35s-LH*

24681012pVS1UbiZmSm/SpepspsP2x35sEPSPSColE1IntTE9TnosintRBLBB513p5o2x35s-Ubi12,419 bp
Promoter 1
2x35s
Gene 1
EPSPS
Promoter 2
UbiZm
Gene 2
MCS
Features
monocot
Size
12,419 bp, circular
Record date
08-FEB-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P2x35s Promoter misc_feature 3141,098785 +
EPSPS Coding sequence CDS 1,1161,856741 +
int Site misc_feature 1,8572,045189 +
epsps Coding sequence CDS 2,0462,888843 +
TE9 Terminator misc_feature 2,8983,188291 +
UbiZm Promoter misc_feature 3,2524,7591,508 +
Int Site misc_feature 4,2384,757520 +
Tnos Terminator misc_feature 4,8205,073254 +
RB T-DNA border misc_feature 5,1965,21924 +
ColE1 Origin / backbone misc_feature 5,7546,451698 +
pVS1 Origin / backbone misc_feature 7,00010,7053,706 +
Sm/Sp Selection marker CDS 10,88911,8991,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.