DNA Cloning Service

Binaries

p5oAct-UbiZm-LH B622

Ligation of 594pUbi-BA* into B621p5oAct-LH2*

24681012pVS1UbiZmACTSm/SpepspsColE1EPSPSintTE9TnoscptintRBLBB622p5oAct-UbiZm13,035 bp
Promoter 1
Act
Gene 1
EPSPS
Promoter 2
UbiZm
Gene 2
MCS
Features
monocot
Size
13,035 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
ACT Promoter misc_feature 3131,7101,398 +
cpt Coding sequence CDS 1,7381,950213 +
EPSPS Coding sequence CDS 1,9512,472522 +
int Site misc_feature 2,4732,661189 +
epsps Coding sequence CDS 2,6623,504843 +
TE9 Terminator misc_feature 3,5143,804291 +
UbiZm Promoter misc_feature 3,8685,3751,508 +
int Site misc_feature 4,8545,373520 +
Tnos Terminator misc_feature 5,4365,689254 +
RB T-DNA border misc_feature 5,8125,83524 +
ColE1 Origin / backbone misc_feature 6,3707,067698 +
pVS1 Origin / backbone misc_feature 7,61611,3213,706 +
Sm/Sp Selection marker CDS 11,50512,5151,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.