p6o2x35s-UbiZm-LGFP C192
Ligation of B633pUbiZm-LGFP* into B483p6o2x35s-LH*
- Promoter 1
- 2x35s
- Gene 1
- hptII
- Promoter 2
- UbiZm
- Gene 2
- link-EGFP
- Features
- monocots
- Size
- 12,720 bp, circular
- Record date
- 29-SEP-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P2x35s | Promoter misc_feature | 314 | 1,098 | 785 | + |
| hptII | Selection marker CDS | 1,116 | 1,685 | 570 | + |
| int | Site misc_feature | 1,686 | 1,874 | 189 | + |
| hptII | Selection marker CDS | 1,875 | 2,330 | 456 | + |
| E9 | Terminator misc_feature | 2,348 | 2,638 | 291 | + |
| Ubi | Promoter misc_feature | 2,693 | 4,201 | 1,509 | + |
| Intron | Site misc_feature | 3,680 | 4,199 | 520 | + |
| link | Site misc_feature | 4,251 | 4,301 | 51 | + |
| GFPm | Coding sequence CDS | 4,302 | 4,680 | 379 | + |
| Int | Site CDS | 4,681 | 4,869 | 189 | + |
| GFPm | Coding sequence CDS | 4,870 | 5,207 | 338 | + |
| OCS | Terminator misc_feature | 5,217 | 5,412 | 196 | + |
| RB | T-DNA border misc_feature | 5,497 | 5,520 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,055 | 6,752 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 7,301 | 11,006 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,190 | 12,200 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.