DNA Cloning Service

Binaries

p6o2x35s-UbiZm-LH B514

Ligation of 594pUbi-BA* into B483p6o2x35s-LH*

1234567891011pVS1UbiZmSm/SpP2x35sColE1hptIIinthptIITE9TnosintRBLBB514p6o2x35s-Ubi11,869 bp
Promoter 1
2x35s
Gene 1
hptII
Promoter 2
UbiZm
Gene 2
MCS
Features
monocot
Size
11,869 bp, circular
Record date
08-FEB-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P2x35s Promoter misc_feature 3141,098785 +
hptII Selection marker CDS 1,1161,685570 +
int Site misc_feature 1,6861,874189 +
hptII Selection marker CDS 1,8752,330456 +
TE9 Terminator misc_feature 2,3482,638291 +
UbiZm Promoter misc_feature 2,7024,2091,508 +
int Site misc_feature 3,6884,207520 +
Tnos Terminator misc_feature 4,2704,523254 +
RB T-DNA border misc_feature 4,6464,66924 +
ColE1 Origin / backbone misc_feature 5,2045,901698 +
pVS1 Origin / backbone misc_feature 6,45010,1553,706 +
Sm/Sp Selection marker CDS 10,33911,3491,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.