p6oAct-UbiZm-oGFPi-LH B616
Ligation of GFP-Hind-Sal* into B615p6oAct-UbiZm-LH*
- Promoter 1
- Act
- Gene 1
- hptII
- Promoter 2
- UbiZm
- Gene 2
- EGFP
- Features
- monocot
- Size
- 13,458 bp, circular
- Record date
- 08-FEB-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| ACT | Promoter misc_feature | 313 | 1,710 | 1,398 | + |
| hptII | Selection marker CDS | 1,738 | 2,307 | 570 | + |
| int | Site misc_feature | 2,308 | 2,496 | 189 | + |
| hptII | Selection marker CDS | 2,497 | 2,952 | 456 | + |
| TE9 | Terminator misc_feature | 2,970 | 3,260 | 291 | + |
| UbiZm | Promoter misc_feature | 3,324 | 4,831 | 1,508 | + |
| int | Site misc_feature | 4,310 | 4,829 | 520 | + |
| link | Other misc_feature | 4,880 | 4,930 | 51 | + |
| gfpm | Coding sequence CDS | 4,931 | 5,309 | 379 | + |
| int | Site CDS | 5,310 | 5,498 | 189 | + |
| gfpm | Coding sequence CDS | 5,499 | 5,836 | 338 | + |
| Tnos | Terminator misc_feature | 5,859 | 6,112 | 254 | + |
| RB | T-DNA border misc_feature | 6,235 | 6,258 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,793 | 7,490 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 8,039 | 11,744 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,928 | 12,938 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.