p6oAct-UbiZm-oGusi-LH C629

Ligation of B497pUbiZm-MoGusi-ocs* into B488p6oAct-LH* sequence

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
pAct	Promoter misc_feature	313	1,710	1,398	+
hptII	Selection marker CDS	1,738	2,307	570	+
int	Site misc_feature	2,308	2,496	189	+
hptII	Selection marker CDS	2,497	2,952	456	+
E9	Terminator misc_feature	2,970	3,260	291	+
Ubi	Promoter misc_feature	3,331	4,838	1,508	+
Int	Site misc_feature	4,317	4,836	520	+
ogus	Coding sequence CDS	4,864	5,478	615	+
unnamed	Site misc_feature	4,960	4,963	4	+
unnamed	Site misc_feature	5,171	5,174	4	+
unnamed	Site misc_feature	5,477	5,480	4	+
'Intron	Site misc_feature	5,479	5,667	189	+
ogus	Coding sequence CDS	5,668	6,864	1,197	+
unnamed	Site misc_feature	5,717	5,720	4	+
unnamed	Site misc_feature	5,756	5,759	4	+
unnamed	Site misc_feature	5,866	5,869	4	+
unnamed	Site misc_feature	6,477	6,479	3	+
unnamed	Site misc_feature	6,500	6,503	4	+
OCS	Terminator misc_feature	6,870	7,065	196	+
RB	T-DNA border misc_feature	7,150	7,173	24	+
ColE1	Origin / backbone misc_feature	7,708	8,405	698	+
pVS1	Origin / backbone misc_feature	8,954	12,659	3,706	+
Sm/Sp	Selection marker CDS	12,843	13,853	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.