p6oAct-oGFPi-LH B888
Ligation of B442pmGFPM-ocs* into B488p6oAct-LH*
- Promoter 1
- Act
- Gene 1
- hptII
- Gene 2
- EGFP
- Features
- monocots
- Size
- 11,779 bp, circular
- Record date
- 04-OCT-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| pAct | Promoter misc_feature | 313 | 1,710 | 1,398 | + |
| hptII | Selection marker CDS | 1,738 | 2,307 | 570 | + |
| int | Site misc_feature | 2,308 | 2,496 | 189 | + |
| hptII | Selection marker CDS | 2,497 | 2,952 | 456 | + |
| E9 | Terminator misc_feature | 2,970 | 3,260 | 291 | + |
| GFP | Coding sequence CDS | 3,362 | 3,740 | 379 | + |
| Int | Site CDS | 3,741 | 3,929 | 189 | + |
| GFP | Coding sequence CDS | 3,930 | 4,267 | 338 | + |
| OCS | Terminator misc_feature | 4,276 | 4,471 | 196 | + |
| RB | T-DNA border misc_feature | 4,556 | 4,579 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,114 | 5,811 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 6,360 | 10,065 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 10,249 | 11,259 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.