DNA Cloning Service

Binaries

p6oAct-oGusi-LH C077

Ligation of B459pMoGusi-ocs* into B488p6oAct-LH* corrected sequence

24681012pVS1pActogusSm/SpColE1ogushptIIhptIIE9Tocs'IntronintRBLBC077p6oAct-oGusi12,871 bp
Promoter 1
Act
Gene 1
hptII
Gene 2
oGus
Features
monocots
Size
12,871 bp, circular
Record date
04-OCT-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
pAct Promoter misc_feature 3131,7101,398 +
hptII Selection marker CDS 1,7382,307570 +
int Site misc_feature 2,3082,496189 +
hptII Selection marker CDS 2,4972,952456 +
E9 Terminator misc_feature 2,9703,260291 +
ogus Coding sequence CDS 3,3623,976615 +
'Intron Site misc_feature 3,9774,165189 +
ogus Coding sequence CDS 4,1665,3621,197 +
Tocs Terminator misc_feature 5,3685,563196 +
RB T-DNA border misc_feature 5,6485,67124 +
ColE1 Origin / backbone misc_feature 6,2066,903698 +
pVS1 Origin / backbone misc_feature 7,45211,1573,706 +
Sm/Sp Selection marker CDS 11,34112,3511,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.