DNA Cloning Service

Binaries

p6oU10-oGFPint B436

Ligation of B444pmGFPD-ocs* into B433p6oU10-LH*

1234567891011pVS1UBQ10Sm/SpColE1hptIIhptIIgfpgfpintTE9bomTocsintintUbi cds 5LBRBB436p6oU10-oGFPi11,838 bp
Promoter 1
Ubq10
Gene 1
hptII
Gene 2
link-EGFP
Features
dicots
Size
11,838 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
hptII Selection marker CDS 1,8042,373570 +
int Site misc_feature 2,3742,562189 +
hptII Selection marker CDS 2,5633,018456 +
TE9 Terminator misc_feature 3,0293,319291 +
gfp Coding sequence CDS 3,4213,799379 +
int Site CDS 3,8003,988189 +
gfp Coding sequence CDS 3,9894,326338 +
Tocs Terminator misc_feature 4,3354,530196 +
RB T-DNA border misc_feature 4,6154,63824 +
ColE1 Origin / backbone misc_feature 5,1735,870698 +
bom Origin / backbone misc_feature 5,9076,166260 +
pVS1 Origin / backbone misc_feature 6,41910,1243,706 +
Sm/Sp Selection marker CDS 10,30811,3181,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.