p6oUZm-ActGFP C076
Ligation of B596pActOs-mGFPM-ocs* into B478p6oUZm-LH*
- Promoter 1
- UbiZm
- Gene 1
- hptII
- Promoter 2
- Act
- Gene 2
- EGFP
- Features
- monocot
- Size
- 13,266 bp, circular
- Record date
- 04-OCT-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| Ubi-int' | Coding sequence CDS | 62 | 1,569 | 1,508 | + |
| Intron | Site misc_feature | 1,049 | 1,568 | 520 | + |
| hptII | Selection marker CDS | 1,590 | 2,159 | 570 | + |
| int | Site misc_feature | 2,160 | 2,348 | 189 | + |
| hptII | Selection marker CDS | 2,349 | 2,804 | 456 | + |
| E9 | Terminator misc_feature | 2,822 | 3,112 | 291 | + |
| pAct | Promoter misc_feature | 3,179 | 4,575 | 1,397 | + |
| GFP | Coding sequence CDS | 4,601 | 4,979 | 379 | + |
| Int | Site CDS | 4,980 | 5,168 | 189 | + |
| GFP | Coding sequence CDS | 5,169 | 5,506 | 338 | + |
| OCS | Terminator misc_feature | 5,515 | 5,710 | 196 | + |
| RB | T-DNA border misc_feature | 5,795 | 5,818 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,353 | 7,050 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 7,599 | 11,304 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,488 | 12,498 | 1,011 | + |
| LB | T-DNA border misc_feature | 13,019 | 13,042 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.