p7o-LH-U10-LGFP C388
Ligation of B631pUbiAt-LGFP* into B414p7o-LH*
- Promoter 1
- 35s
- Gene 1
- bar
- Promoter 2
- Ubq10
- Gene 2
- link-EGFP
- Features
- dicot
- Size
- 11,828 bp, circular
- Record date
- 29-SEP-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| Bar | Selection marker CDS | 745 | 978 | 234 | + |
| STLS1 | Other misc_feature | 979 | 1,167 | 189 | + |
| Bar | Selection marker CDS | 1,168 | 1,483 | 316 | + |
| E9 | Terminator misc_feature | 1,496 | 1,786 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 1,835 | 3,310 | 1,476 | + |
| Int | Site misc_feature | 3,005 | 3,308 | 304 | + |
| link | Site misc_feature | 3,359 | 3,409 | 51 | + |
| GFPm | Coding sequence CDS | 3,410 | 3,788 | 379 | + |
| Int | Site CDS | 3,789 | 3,977 | 189 | + |
| GFPm | Coding sequence CDS | 3,978 | 4,315 | 338 | + |
| OCS | Terminator misc_feature | 4,325 | 4,520 | 196 | + |
| RB | T-DNA border misc_feature | 4,605 | 4,628 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,163 | 5,860 | 698 | + |
| bom | Origin / backbone misc_feature | 5,897 | 6,156 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 6,409 | 10,114 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 10,298 | 11,308 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.