DNA Cloning Service

Binaries

p7o-LH-U10-LGFP C388

Ligation of B631pUbiAt-LGFP* into B414p7o-LH*

1234567891011pVS1Pro-Ubq10AtSm/SpColE135SGFPmGFPmBarIntE9bomBarOCSIntSTLS1linkRBLBC388p7o-LH-U10-L11,828 bp
Promoter 1
35s
Gene 1
bar
Promoter 2
Ubq10
Gene 2
link-EGFP
Features
dicot
Size
11,828 bp, circular
Record date
29-SEP-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
35S Promoter misc_feature 309724416 +
Bar Selection marker CDS 745978234 +
STLS1 Other misc_feature 9791,167189 +
Bar Selection marker CDS 1,1681,483316 +
E9 Terminator misc_feature 1,4961,786291 +
Pro-Ubq10At Promoter misc_feature 1,8353,3101,476 +
Int Site misc_feature 3,0053,308304 +
link Site misc_feature 3,3593,40951 +
GFPm Coding sequence CDS 3,4103,788379 +
Int Site CDS 3,7893,977189 +
GFPm Coding sequence CDS 3,9784,315338 +
OCS Terminator misc_feature 4,3254,520196 +
RB T-DNA border misc_feature 4,6054,62824 +
ColE1 Origin / backbone misc_feature 5,1635,860698 +
bom Origin / backbone misc_feature 5,8976,156260 +
pVS1 Origin / backbone misc_feature 6,40910,1143,706 +
Sm/Sp Selection marker CDS 10,29811,3081,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.