p7o2x35s-UbiZm-mGFP B890
Ligation of B443pmGFPM-nos* into B515p7o2x35s-UbiZm-LH*
- Promoter 1
- 2x35s
- Gene 1
- bar
- Promoter 2
- UbiZm
- Gene 2
- EGFP
- Features
- monocots
- Size
- 12,317 bp, circular
- Record date
- 29-SEP-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P2x35s | Promoter misc_feature | 314 | 1,098 | 785 | + |
| Bar | Selection marker CDS | 1,116 | 1,349 | 234 | + |
| STLS1 | Other misc_feature | 1,350 | 1,538 | 189 | + |
| Bar | Selection marker CDS | 1,539 | 1,856 | 318 | + |
| E9 | Terminator misc_feature | 1,876 | 2,166 | 291 | + |
| Ubi-Prot | Promoter misc_feature | 2,230 | 3,737 | 1,508 | + |
| Intron | Site misc_feature | 3,216 | 3,735 | 520 | + |
| GFP | Coding sequence CDS | 3,791 | 4,169 | 379 | + |
| Int | Site CDS | 4,170 | 4,358 | 189 | + |
| GFP | Coding sequence CDS | 4,359 | 4,696 | 338 | + |
| NOS-T | Terminator misc_feature | 4,718 | 4,971 | 254 | + |
| RB | T-DNA border misc_feature | 5,094 | 5,117 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,652 | 6,349 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 6,898 | 10,603 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 10,787 | 11,797 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.