p7o2x35s-UbiZmF-LGFP B712
Ligation of B634pUbiZmF-LGFP* into B484p7o2x35s-LH*
- Promoter 1
- 2x35s
- Gene 1
- bar
- Promoter 2
- UbiZm
- Gene 2
- link-EGFP
- Features
- monocot
- Size
- 12,735 bp, circular
- Record date
- 08-FEB-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P2x35s | Promoter misc_feature | 314 | 1,098 | 785 | + |
| bar | Selection marker CDS | 1,116 | 1,349 | 234 | + |
| int | Site misc_feature | 1,350 | 1,538 | 189 | + |
| bar | Selection marker CDS | 1,539 | 1,856 | 318 | + |
| TE9 | Terminator misc_feature | 1,876 | 2,166 | 291 | + |
| UbiZmF | Promoter misc_feature | 2,228 | 4,221 | 1,994 | + |
| link | Other misc_feature | 4,266 | 4,316 | 51 | + |
| gfpm | Coding sequence CDS | 4,317 | 4,695 | 379 | + |
| int | Site CDS | 4,696 | 4,884 | 189 | + |
| gfpm | Coding sequence CDS | 4,885 | 5,222 | 338 | + |
| Tocs | Terminator misc_feature | 5,232 | 5,427 | 196 | + |
| RB | T-DNA border misc_feature | 5,512 | 5,535 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,070 | 6,767 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 7,316 | 11,021 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,205 | 12,215 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.